Alterations in Electrocardiographic Parameters and Serum Cardiac Biomarkers in an Ovine Experimental Endotoxemia Model

Abstract

In order to study the cardiac health status based on electrocardiogram recording and measurement of circulating cardiac biomarkers following the induction of endotoxemia, 5 clinically healthy 1-year old Iranian fat-tailed ewes (25±1.5 kg, bodyweight) were randomly selected and lipopolysaccharide from Escherichia coli serotype O55:B5 was used to induce endotoxemia in ewes at 20 µg/kg. The electrocardiograms and blood samples were taken prior and 1, 2, 3, 4, 5, 6 and 24 hours after lipopolysaccharide injection. Values of serum homocysteine, cardiac troponin I, creatine kinase isoenzyme MB and lactate dehydrogenase were assayed during the study. The rapid and significant elevation of homocysteine, cardiac troponin I, creatine kinase isoenzyme MB and lactate dehydrogenase was seen after endotoxemia induction (P<0.05). The results showed that T duration increased significantly after endotoxemia induction and decreased near to base line levels at 6^th hour after lipopolysaccharide infusion. T amplitude decreased significantly after endotoxemia induction. The significant increase in R-R, S-T and Q-T intervals were detected during endotoxemia. In conclusion, it seems that endotoxemia causes myocardial autonomic dysfunction and significant changes in ECG parameters could be interpreted in the light of concurrent cardiac biomarker changes.

Key Words: Endotoxemia, electrocardiogram, cardiac biomarkers, Iranian fat-tailed sheep

 

Keywords

• Endotoxemia, electrocardiogram, cardiac biomarkers, Iranian fat-tailed sheep

KOYUN DENEYSEL ENDOTOKSEMİ MODELİNDE ELEKTROKARDİYOGRAFİ PARAMETRELERİNDEKİ VE SERUM KARDİYAK BİYOMARKIRLARDAKİ DEĞİŞİMLER

Abstract

Endotokseminin indüklemesini takiben elektrokardiyografi kayıtlarına ve dolaşımdaki kardiyak biyomarkerların ölçümüne dayandırılarak kardiyak sağlık durumu çalışılmıştır. Klinik açıdan sağlıklı 1 yaşındaki İran yağlı kuyruk
5 koyun (25±1,5 kg, ağırlık) rastgele seçilmiştir ve bu koyunlara endotoksemi oluşturmak amacıyla 20 µg/kg’dan Escherichia coli O55:B5 serotipinden lipopolisakkarid kullanılmıştır. Elektrokardiyogramlar ve kan örnekleri lipopolisakkarid enjeksiyonunun öncesinde ve 1, 2, 3, 4, 5, 6 ve 24 saat sonrasında alınmıştır. Çalışma süresince serum homocysteine, kardiyak troponin I, creatine kinase izoenzimi MB ve laktat dehidrojenaz seviyeleri ölçülmüştür. Endoksemi oluşturulduktan sonra homocysteine, kardiyak troponin I, creatine kinase izoenzimi MB ve laktat dehidrojenaz seviyelerinde hızlı ve belirgin bir yükselme (P<0,05) görülmüştür. Sonuçlar endotoksemi oluştuktan sonra T zamanının belirgin bir şekilde artığını ve lipopolisakkarid enjeksiyonundan 6 saat sonra alt çizgisiye kadar düşmüştür. T amplitüdü endotoksemi oluştuktan sonra belirgin şekilde düşer. Endotoksemi süresince R-R, S-T ve Q-T aralıklarında belirgin bir şekilde yükselme belirlenmiştir. Sonuç olarak, endotoksemi myokardiyal otonomik fonksiyon bozukluğuna neden olur ve EKG parametrelerinde belirgin değişimler aynı zamanda yapılan kardiyak biyomarkerlar ile yorumlanabilir.

Anahtar Kelimeler: Endotoksemi, elektrokardiyografi, kardiyak biyomarkerlar, İran yağlı kuyruklu koyunu

Keywords

• Endotoksemi, elektrokardiyografi, kardiyak biyomarkerlar, İran yağlı kuyruklu koyunu

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