Authentication of Meat Species in Sucuk by Multiplex PCR

Abstract

The identification of meat species used in meat products is important by reason of economic considerations, religious factors, verification of label, and prevention of unfair-market competition. In this paper, multiplex PCR method was experienced for routine detection of equine (horse and donkey), poultry (chicken and turkey), pig and cattle meat in sucuk (sausage). The primers used for these animals generated specific fragments, and they did not show cross reactions with the DNA from the other genus of animal. After multiplex PCR was successfully optimized, a field study was carried out to investigate the presence of horse, donkey, chicken, turkey and pig meat in 50 sucuks (30 beef and 20 beef + poultry) collected from markets. The result of the field study indicated that 23.3% of 30 beef sucuk samples were containing poultry meat. None of the 50 sucuk samples was containing pig meat, but one (2%) of the samples generated equine fragment. The present study showed that the multiplex PCR method can be used for routine analysis of meat species identification, verification and control of label information of meat products.

Keywords

• Multiplex PCR, species identification, sucuk

Multipleks PCR ile Sucuklarda Et Türlerinin Doğruluğunun Kanıtlanması

Abstract

Et ürünlerinde kullanılan et türlerinin saptanması ekonomik sebepler, dini faktörler, etiketin doğrulanması ve haksız rekabetin önlenmesi açısından önemlidir. Bu çalışmada, sucuklarda tek tırnaklı (at ve eşek), kanatlı (tavuk ve hindi), domuz ve sığır etinin rutin tespiti için multipleks PCR metodu denendi. Bu hayvanların DNA’larının tespitinde diğer hayvan türleri ile çapraz eşleşme göstermeyen türe özgü primerler kullanıldı. Multipleks PCR başarılı bir şekilde optimize edildikten sonra, marketlerden toplanmış 50 adet sucukta (30 adeti sığır eti, 20 adeti sığır eti-kanatlı eti karışımı) at, eşek, tavuk, hindi ve domuz etinin varlığının araştırıldığı bir saha çalışması gerçekleştirildi. Saha çalışmasının sonuçları sığır etinden üretilmiş 30 adet sucuğun %23,3’ünün kanatlı eti içerdiğini gösterdi. Elli adet sucuk örneğinin hiçbirinde domuz eti bulunmadı fakat bir örnekten (%2) tek tırnaklı DNA parçası çoğaltıldı. Bu çalışma multipleks PCR metodunun et ürünlerinde et türlerinin belirlenmesi, doğrulanması ve etiket bilgilerinin kontrolü için rutin olarak kullanılabileceğini gösterdi.

Keywords

• Multipleks PCR, tür tayini, sucuk

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