Assessment of relationship between IGF-I concentration before parturition and postpartum endometritis and ovarian cysts in dairy cattle

Abstract

This study was carried out to assess the IGF-I, non-esterified fatty acids (NEFA), beta hydroxybutyrate (BHB) and glucose concentrations during the peripartum period of dairy cows in normal condition, and cows with endometritis puerperalis and cystic ovarian disease.  The study was conducted on 87 lactating Holstein cows (parity: 1-7) in the 9th month of pregnancy in Shiraz, Iran. Blood samples were collected every 2 weeks from 2 weeks before until 6 weeks after calving. Two, four and six weeks after calving, palpation of the reproductive tract was performed. Cows were first inspected for the presence of fresh abnormal discharge on the vulva, perineum, or tail then those were examined intra-vaginally. Following inspection, ultrasonographic assessment of uterus and ovaries was also performed and ovarian structures (palpable follicle, CL and cyst) were scanned. Prepartum IGF-I concentration was significantly lower in cows that developed cystic ovaries than non-cystic (35.89±9.09 versus 41.99±3.65 μg/L) and cows with clinical endometritis early postpartum than normal cows (36.65±5.38 versus 43.64±4.32 μg/L). Calving-first service interval and ovarian cycle resumption after calving was significantly shorter for cows without clinical endometritis than cows with clinical endometritis. In conclusion, prepartum IGF-I concentration was the main factor associated with occurrence of endometritis and cystic ovarian disease after calving and it was a notable feature of the current study.

Keywords

• IGF-I, endometritis, ovarian cyst, prepartum, holstein, dairy cows

Sütçü İneklerde Doğum Öncesi ve Postpartum Endometritis ve Yumurtalık Kistlerinde IGF-I Düzeylerindeki İlişkinin Değerlendirilmesi

Abstract

Bu çalışma peripartum dönem sırasında normal durumdaki sütçü inekler ile puerperal endometritis ve yumurtalık kisti olan ineklerdeki IGF-1, esterleşmemiş yağ asitleri (NEFA), beta hidroksibutirat (BHB) ve glukoz düzeylerinin değerlendirilmesi için gerçekleştirilmiştir. Çalışma İran, Şiraz’da gebeliklerinin 9. ayında bulunan 87 adet laktasyondaki Siyah Alaca (doğum sayısı: 1-7) inekte yürütülmüştür. Buzağı doğumlarından 2 hafta öncesinden başlayarak doğumdan 6 hafta sonrasına kadar her 2 haftada bir kan örnekleri alınmıştır. Buzağı doğumlarından iki, dört ve altı hafta sonra ineklerin genital organlarının durumları rektal palpasyon ile değerlendirilmiştir. İneklerin vulva, perineum ya da kuyruk bölgesinde taze anormal akıntı varlığı yönünden incelendikten sonra intravajinal incelemesi de yapılmıştır. İnceleme sonrası, rahim ve yumurtalıkların ultrasonografik değerlendirilmesi yapılmış ve yumurtalık yapıları (palpe edilebilen folikül, CL ve kist) taranmıştır. Prepartum IGF-I düzeyleri kistik yumurtalıkları oluşan ineklerde kistik olmayanlara göre (35,89±9,09’a karşı 41,99±3,65 μg/L) ve erken postpartum dönemde klinik endometritli ineklerde sağlıklı ineklere göre (36,65±5,38’a karşı 43,64±4,32 μg/L) anlamlı olarak daha düşük bulunmuştur. Klinik yönden endometritli olmayan ineklerde doğum sonrası ilk tohumlama aralığı ve doğum sonrası östrus siklusunun yeniden başlama sürelerinin klinik yönden endometritli ineklere göre anlamlı olarak daha kısa bulunmuştur. Sonuç olarak, prepartum IGF-I düzeyi doğum sonrası endometritis ve kistik ovaryumun oluşumu ile ilişkili temel faktördür ve ortaya konulan bu sonuç çalışmanın önemli bir özelliğidir.

Keywords

• IGF-I, endometrit, yumurtalık kisti, prepartum, siyah alaca, süt sığırı

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