Protective Effects of Vitamin C (Ascorbic Acid) in Lead Acetate Exposed Diabetic Male Rats: Evaluation of Blood Biochemical Parameters and Testicular Histopathology

Abstract

The aim of this study was to investigate the protective effects of vitamin C against lead toxicity by measuring the blood parameters and studying histopathology of testis in diabetic male rats. Wister rats (42) were randomly assigned into7 groups: I) healthy; II) fed lead acetate only; III) vitamin C administered only; IV) diabetic; V) diabetic rats administered by vitamin C; VI) diabetic rats given lead acetate and VII) diabetic rats received lead acetate and vitamin C. The diabetic and lead groups had higher glucose, cholesterol, LDL, triglycerides and lower insulin and HDL concentration than the control group. It was found that vitamin C administration led to a lower level of blood glucose, cholesterol, LDL and triglycerides and higher HDL concentration in diabetic rats significantly. It was concluded that the antioxidant property of vitamin C resulted in reducing the oxidative stress complications of toxic levels of lead acetate in diabetic rats.

Keywords

• Antioxidant, diabetes, lead, toxicity, vitamin c, Wistar rats

Kurşun Asetata Maruz Kalan Diyabetik Erkek Sıçanlarda Vitamin C (Askorbik Asit)’nin Koruyucu Etkisi: Kan Biyokimyasal Parametreleri ve Testis Histopatolojisinin Değerlendirilmesi

Abstract

Bu çalışmanın amacı diyabetik erkek sıçanlarda kan parametrelerinin ölçülmesi ve testisin histopatolojisinin çalışılması ile vitamin C’nin kurşun toksisitesine karşı koruyucu etkisini incelemektir. Wistar sıçanlar (42) rastgele 7 gruba ayrılmıştır I) sağlıklı; II) sadece kurşun asetat ile beslenenler; III) sadece vitamin C uygulananlar; IV) diyabetik; V) vitamin C uygulanan diyabetik sıçanlar; VI) kurşun asetat verilen diyabetik sıçanlar ve VII) kurşun asetat ve vitamin C uygulanan diyabetik sıçanlar. Diyabetik ve kurşun verilen grupların kontrol gruplara kıyasla daha yüksek glukoz, kolesterol, LDL, trigliseritler ve daha düşük insülin ve HDL konsantrasyonuna sahip oldukları tespit edilmiştir. Vitamin C uygulaması kanda glukoz, kolesterol, LDL ve trigliserit düzeylerini düşürdüğü HDL konsantrasyonunu ise arttırdığı saptanmıştır. Sonuç olarak diyabetik farelerde vitamin C’nin antioksidan özelliği, kurşun asetatın toksik seviyelerinde oksidatif stres komplikasyonlarını azaltmasına neden olmuştur.

Keywords

• Antioksidan, diyabet, kurşun, toksisite, vitamin C, Wistar sıçanlar

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