Localizations of α-SMA, S-100 Protein and Their Subunits in the Lacrimal Glands of the Rats

Abstract

This study is intended to reveal immunohistochemical localizations of α-SMA and calcium binding wbS-100, S-100α and S-100β proteins in intraorbital and extraorbital lacrimal gland of adult male rats. After extraorbital and intraorbital sections of the lacrimal gland were extracted from the rats under anesthesia, Streptavidin-biotin-peroxidase immunohistochemical technique to determine wbS-100, S-100α, S-100β and α-SMA proteins were applied to the 4-μm sections taken from the tissue samples blocked following the routine histological procedure. Immunoreaction of wbS-100, S-100α and S-100β was determined both in cytoplasm and nucleus of the acinus epithelium, duct epithelium, myoepithelial and endothelial cells of extraorbital lacrimal gland. While S-100α immunoreaction in all structural components of the intraorbital gland was most densely observed in the nucleus, S-100β immunoreaction was negative in myoepithelial cells. Moreover, it is detected that wbS-100 immunreaction was positive in the lateral membranes of acinus epithelial cells of extraorbital gland and wbS100 and S-100β immunoreaction was positive in the lateral membranes of acinus and duct epithelial cells of intraorbital gland. α-SMA immunoreaction was detected in the myoepithelial cells and the smooth muscle cells in the wall of blood vessels of both glands. In conclusion, the differences detected between wbS-100, S-100α and S-100β proteins in all structural components of intraorbital and extraorbital lacrimal glands in terms of immunohistochemical staining density suggest that there is a functional difference between both lacrimal glands with respect to the secretory activity.

Sıçanların Lakrimal Bezlerinde α-SMA, S-100 Protein ve Alt Ünitelerinin Lokalizasyonları

Abstract

Bu çalışma, erişkin erkek sıçanların intraorbital ve ekstraorbital lakrimal bezinde kalsiyum bağlayan wbS-100, S-100α ve S-100β proteinleri ile α-SMA’nın immuno-histokimyasal lokalizasyonlarını ortaya koymak amacıyla planlandı. Sıçanlardan anestezi altında lakrimal bezin ekstraorbital ve intraorbital kısımları çıkartıldıktan sonra rutin histolojik prosedürü takiben bloklanan doku örneklerinden alınan 4 μm kalınlığındaki kesitlere wbS-100, S-100α, S-100β ve α-SMA proteinlerini belirlemek için Streptavidin-biotin-peroksidaz immunohistokimyasal teknik uygulandı. Ekstraorbital lakrimal bezin asinus epiteli, akıtıcı kanal epiteli, miyoepitel ve endotel hücrelerinin hem sitoplazmasında hem de çekirdeğinde wbS-100, S-100α ve S-100β immunreaksiyonuna rastlandı. İntraorbital bezin bütün yapısal komponentlerinde S-100α immunreaksiyonu en yoğun derecede çekirdekte gözlenirken, S-100β immunreaksiyonu miyoepitel hücrelerinde negatifti. Aynı zamanda ekstraorbital bezin asinus epitel hücrelerinin lateral membranlarında wbS-100 immunreaksiyonu, intraorbital bezin asinus epiteli ve akıtıcı kanal epitel hücrelerinin lateral membranlarında ise wbS100 ve S-100β immunreaksiyonu belirlendi. Her iki bezin miyoepitel hücreleri ile kan damarlarının duvarında yer alan düz kas hücrelerinde α-SMA immunreaksiyonu saptandı. Sonuç olarak, intraorbital ve ekstraorbital lakrimal bezlerin bütün yapısal komponentlerinde wbS-100, S-100α ve S-100β proteinleri arasındaki immunohistokimyasal boyanma yoğunluğu açısından tespit edilen bu farklılıkların her iki lakrimal bez arasında salgı aktivitesi ile ilgili fonksiyonel farklılığın olduğu düşüncesini akla getirmektedir.

Keywords

• α-SMA, lakrimal bez, S-100 protein, sıçan

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