Identification of Allele Frequency of Factor XI Deficiency (FXID) in Holstein Cows Reared in Thrace District of Turkey

Abstract

Factor XI (FXI) is a protein that plays a key role in plasma coagulation. Factor XI Deficiency (FXID) is an autosomal recessive disease caused by an insertion into exon 12 of FXI gene. The aim of this study is to determine the allele frequency of Factor XI Deficiency (FXID) in Holstein cows reared in Thrace region of Turkey. Blood samples of 287 Holstein cows were used for DNA isolation. Amplification of FXI gene was followed by the evaluation of PCR products with visualization on 2% agarose gel electrophoresis. FXID mutant allele was not observed in any of the samples used in this study. In conclusion, none of the Holstein cows were neither affected nor carriers for FXID among all analysed Holstein cows reared in Thrace region of Turkey.

Türkiye’de Trakya Yöresinde Yetiştirilen Holştayn İneklerde Faktör XI Yetmezliği (FXID) Allel Frekansının Belirlenmesi

Abstract

Faktör XI (FXI) plazma pıhtılaşmasında görev alan önemli bir proteindir. FXI eksikliği (FXID) FXI geninin 12. ekzonunda meydana gelen bir insersiyon sonucu oluşan otozomal resesif bir hastalıktır. Bu çalışmanın amacı Türkiye’nin Trakya bölgesinde yetiştirilen Holştayn ineklerde FXID allel frekansının belirlenmesidir. DNA izolasyonu 287 Holştayn ineğine ait kan örneklerinden yapılmıştır. FXI geninin polimeraz zincir reaksiyonu (PZR) ile çoğaltılmasını takiben oluşan ürünler %2’lik agaroz jel elektroforezi ile görüntülenerek değerlendirilmiştir. Örneklerin hiçbirinde FXID mutant alleli gözlenmemiştir. Sonuç olarak Trakya bölgesinde taranan tüm Holştayn ineklerde FXID’den etkilenen veya taşıyıcısı olan inek tespit edilmemiştir.

Keywords

• Holştayn, Trakya, FXID, genetik hastalık

References

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