DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE

Year 2008, Volume: 34 Issue: 2, 7 - 13, 21.11.2012

Göknur Terzi Ufuk Kamber Cevat Nisbet Sena Çenesiz

Abstract

ABSTRACT

Vitamin B₁₂ is a member of the vitamin B complex. It contains cobalt, and is also known as cobalamin. It is exclusively synthesised by bacteria and is found primarily in fish, meat, poultry, eggs, milk and milk products. Vitamin B₁₂ deficiency have an underlying stomach or intestinal disorder that limits the absorption of vitamin B₁₂ and might be caused to megaloblastic anemia. In this study cow milk samples were collected from in two different region in Turkey. In milk samples the amount of vitamin B₁₂ was detected by using ELISA. The vitamin B₁₂ concentrations of milk samples obtained from the cows of Holstein, Jersey, Zavot and East Anatolian Red species were determined as 0.98µg/L, 1.15µg/L, 2.93µg/L and 1.56µg/L, respectively. The concentrations of vitamin B₁₂ in Zavot cows milk were higher than the other species.

Key words: Vitamin B₁₂,  cow milk, ELISA.

 

FARKLI SIĞIR IRKLARINA AİT SÜT ÖRNEKLERİNDE B 12 VİTAMİN DÜZEYİNİN ELİSA TEKNİĞİ İLE TESPİTİ

ÖZET

B₁₂ vitamini, B kompleks vitaminlerin bir üyesidir. Bu vitamin kobalt içerdiğinden dolayı kobalamin olarak adlandırılmaktadır. B₁₂ vitamini balık, et, tavuk, yumurta ve süt ürünlerinde bulunur ve bakteriler tarafından sentezlenir. B₁₂ vitamini yetersizliğine bağlı olarak mide ve bağırsak bozukluklar, kol ve bacaklarda uyuşukluk ve megolablastik anemi şekillenir. Bu çalışmada farklı sığır ırklarına ait süt örneklerinde B₁₂ vitamin düzeyleri araştırıldı. Bu amaçla Samsun ve Kars bölgesinden Holştayn, Jersey, Zavot and Doğu Anadolu Kırmızısı sığır ırklarına ait süt örnekleri toplandı. Süt örneklerinde B₁₂ vitamini miktarı ELISA yöntemi ile tespit edildi. B₁₂ vitamini Holştayn, Jersey, Zavot and Doğu Anadolu Kırmızısı sığır ırklarına ait süt örneklerinde sırasıyla 0.98µg/L, 1.15µg/L, 2.93µg/L ve 1.56µg/L olarak bulundu. Yapılan istatistik çalışması sonucunda vitamin B₁₂ miktarı Zavot ırkına ait süt örneklerinde diğer ırklardan daha yüksek bulundu (p<0.05, ANOVA).

Anahtar Kelimeler: B₁₂ vitamini, inek sütü, ELISA.

Keywords

B1 2 vitamini, inek sütü, ELISA

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