MANDA KARKASLARI VE REKTAL SWABLARINDA Escherichia coli O157:H7 VARLIĞININ ARAŞTIRILMASI

Year 2007, Volume: 33 Issue: 3, 59 - 65, 29.11.2012

Aysun Yılmaz Hüseyin Gün

Abstract

ÖZET

Bu çalışmanın amacı mandalardan alınan rektal swab örneklerinde ve manda karkaslarında E. coli O157:H7 varlığının araştırılmasıdır. Bu amaçla, İstanbul'da bulunan 5 mezbaha Ocak 2001 ve Ocak 2002 tarihleri arasında ziyaret edildi. Sistematik örnekleme yöntemiyle toplam 28 mandanın rektumlarından ve karkaslarından swablar alindi. Alınan swablar Novobiocin içeren modifiye E. coli broth içine konuldu. İnkubasyondan sonra immunomanyetik separasyon (IMS) yapıldı ve IMS urunu daha sonraki identifikasyon çalışmaları için Cefixime Tellurite MacConkey (CT-SMAC) agara ekildi. İdentifikasyon amacıyla krom agara ekimler, biyokimyasal ve serolojik testler yapıldı. İzolatların anti-O157 ve H7 serumu ile reaksiyonu incelendi. Rektal swablardan izole edilen 7 ve karkaslardan izole edilen 2 izolat CT-SMAC agarda sorbitol negatif koloniler oluşturdu. Bu izolatların hiçbirisi E. coli O157 ve H7 antiserumu ile aglutinasyon oluşturmadı.

Anahtar Kelimeler: Manda, karkas, mezbaha, E. coli O157:H7, İstanbul

 

INVESTIGATIONS ON THE PRESENCE OF ESCHERICHIA COLI O157:H7 ON BUFFALO CARCASSES AND RECTAL SWABS

ABSTRACT

The aim of this study was to investigate presence of E. coli O157:H7 on rectal swabs and buffalo carcasses slaughtered in the abattoirs. Five abattoirs in Istanbul were visited between January 2000 and 2001. During visits, systematic sampling method was used and a total of 28 buffalo and their carcasses were sampled. All the swabs were placed in modified E. coli broth containing Novobiocin. Immunomagnetic seperation (IMS) was performed after the preenrichment. The product from the IMS was used for further identification procedures using the Cefixime Tellurite MacConkey agar (CT-SMAC) agar, Chrom agar and biochemical tests. The reaction of the isolates with anti-O157 and H7 sera were also analysed. Seven isolates from the rectal swabs and 2 from the carcasses were sorbitol negative on CT-SMAC agar. None of these isolates reacted with antisera to E. coli O157 nd H7.

Key Words: Buffalo, carcasses, abattoir, E. coli O157:H7, Istanbul

Keywords

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