EcoRI Polymorphism in Intron 6 of the Bovine Lactoferrin Gene in South Anatolian Red and East Anatolian Red Cattle Breeds

Year 2013, Volume: 39 Issue: 2, 183 - 188, 10.03.2013

Feraye Esen Gürsel , İraz Akış , Atila Ateş , Hasret Yardibi , Gülhan Türkay Hoştürk Kemal Öztabak

Abstract

ABSTRACT

Lactoferrin (LF) is an iron-binding glycoprotein that exists in variety of biological fluids such as milk, tear, saliva, bile, mucous secretions and blood. Bovine mastitis is an inflammation of the mammary gland. LF has a vital role in the defense mechanism of the mammary gland and the bovine LF gene is a potential candidate gene for imparting resistance to mastitis in dairy cows. The objective of the study is to determine the genotype and allele frequencies for bovine lactoferrin gene polymorphisms in South Anatolian Red (SAR) and East Anatolian Red (EAR) cattle. DNA samples of 46 SAR and 46 EAR cattle were selected from DNA collection obtained from previous research projects. Bovine LF gene was amplified by polymerase chain reaction (PCR) and the PCR products were digested by EcoRI restriction enzyme. The frequency of A allele was found higher than B allele in EAR cattle breed and the frequency of B allele was found higher than A allele in SAR cattle breed. We suggest that further studies should be conducted on the bovine LF gene in Bos taurus and Bos indicus cattle to understand the reason of high genotype frequency in SAR and EAR cattle breeds.

Key Words: Bovine Lactoferrin gene, South Anatolian Red cattle, East Anatolian Red cattle

 

 

ÖZET

 

DOĞU ANADOLU KIRMIZISI VE GÜNEY ANADOLU KIRMIZISI IRKI SIĞIRLARDA
LAKTOFERRİN GENİNİN 6. İNTRONUNDAKİ ECORI POLİMORFİZMİ

 

Laktoferrin (LF) süt, gözyaşı, tükürük, safra, mukus salgıları ve kan gibi çeşitli biyolojik sıvılarda bulunan, demir bağlayıcı özelliği olan bir glukoproteindir. LF meme bezi savunma mekanizmasında önemli bir role sahiptir ve sığır laktoferrin geni sütçü ineklerin mastite karşı direnç şekillenmesinde potansiyel aday bir gendir. Bu çalışmada Güney Anadolu Kırmızısı (GAK) ve Doğu Anadolu Kırmızısı (DAK) sığırlarında, laktoferrin genindeki polimorfizmlerin genotip ve allel dağılımlarının belirlenmesi amaçlanmıştır. Çalışmada kullanılan 46 DAK ve 46 GAK DNA örneği, önceki yapılan çalışmalar sonucu elde ettiğimiz DNA koleksiyonundan temin edilmiştir. Hedef bölge polimeraz zincir reaksiyonu (PZR) ile çoğaltılmış ve PZR ürünleri EcoRI restriksiyon enzimi ile kesilmiştir. DAK ırkı sığırlarda A allelinin frekansının B allelinin frekansına oranla yüksek olduğu ve GAK ırkı sığırlarda ise B allelinin frekansının A allelinin frekansına oranla yüksek olduğu tespit edilmiştir. GAK ve DAK sığır ırklarında tespit edilen yüksek genotip frekansının sebebinin daha iyi anlaşılabilmesi için Bos taurus and Bos indicus ırkı sığırlarında laktoferrin genine yönelik çalışmaların yapılmasını önermekteyiz.

Anahtar Kelimeler: Sığır Laktoferrin geni, Güney Anadolu Kırmızısı, Doğu Anadolu Kırmızısı 

Keywords

Sığır Laktoferrin geni, Güney Anadolu Kırmızısı, Doğu Anadolu Kırmızısı

References

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