Comparison of Certain Haematological and Biochemical Parameters Regarding Pre-slaughter Stress in Saanen, Maltese, Gokceada and Hair Goat Kids

Year 2013, Volume: 39 Issue: 2, 189 - 196, 10.03.2013

Elif Ergul Ekız Hulya Yalcıntan

Abstract

ABSTRACT

In farm animals, pre-slaughter period is the most stressful stage of their lifetime. The current study was conducted to investigate comparatively the stress responses of Saanen, Maltese, Gokceada and Hair Goat kids to the stressors related with pre-slaughter management procedures. A total of 40 goat kids from 4 different breeds (11 Saanen,
11 Maltese, 9 Gokceada and 9 Hair Goat) were used in the study. Two blood samples (EDTA and heparinised) were collected from each animal at two different times (at home pen and at exsanguination). Packed cell volume (PCV) was measured in EDTA blood samples by the standard capillary microhematocrite method. Heparinised samples were used for determination of plasma glucose, lactate dehydrogenase (LDH), creatine kinase (CK), cortisol and total protein concentrations. For each breed, plasma glucose and cortisol concentrations measured at exsanguination were higher than those of determined at home pen. Breed had no significant effect on plasma glucose and cortisol concentrations at both sampling times (P>0.05). However, Maltese kids had higher concentration of plasma LDH at exsanguination than Saanen kids (P<0.05), and they had higher plasma CK level than Gokceada, Saanen and Hair Goat kids (P<0.05). The effect of breed on PCV and total protein level was significant at both sampling times. Plasma total protein concentration measured at home pen and at exsanguination in Hair Goat kids was lower than those measured in other breeds (P<0.01). It is concluded that, pre-slaughter procedures resulted in a stress response in Saanen, Maltese, Gokceada and Hair Goat kids. However, breed had no significant effect on cortisol and glucose responses of Goat kids to the stressors related with pre-slaughter management procedures. Moreover, significant differences were observed among breeds in terms of CK and LDH levels measured at exsanguination, which is probably resulted from the difference in temperament.

Key Words: Goat kid, breed effect, stress parameters at exsanguination

 

 

ÖZET

 

SAANEN, MALTA, GÖKÇEADA VE KIL KEÇİSİ IRKI OĞLAKLARDA KESİM STRESİNE İLİŞKİN BAZI HEMATOLOJİK VE BİYOKİMYASAL PARAMETRELERİN KARŞILAŞTIRILMASI

 

Çiftlik hayvanlarında kesim öncesi dönem hayatlarının en stresli dönemidir. Bu araştırmada Saanen, Malta, Gökçeada ve Kıl Keçisi ırkı oğlakların kesim öncesi uygulanan prosedürlere karşı verdikleri stres yanıtının karşılaştırmalı olarak incelenmesi amaçlanmıştır. Araştırmada dört farklı ırktan toplam 40 adet erkek oğlak
(11 Saanen, 11 Malta, 9 Gökçeada, 9 Kıl Keçisi) kullanılmıştır. Her bir hayvandan, hem oğlaklar besi bölmesinde iken hem de kesim sırasında ikişer adet kan örneği (EDTA'lı ve heparinli) alınmıştır. Hematokrit değer (PCV) EDTA'lı kan örneklerinde standart kapillar mikrohematokrit yöntemi ile belirlenmiştir. Plazma kortizol, glukoz, kreatinin kinaz (CK), laktat dehidrojenaz (LDH) ve total protein, düzeyleri ise heparinli kan örneklerinde belirlenmiştir. Araştırma kapsamında incelenen her bir keçi ırkı için kesim sırasında belirlenen plazma kortizol ve glukoz düzeyleri hayvanlar besi bölmesinde iken yapılan ölçümlerden daha yüksek bulunmuştur. Her iki kan alma döneminde de ırkın plazma kortizol ve glukoz düzeyleri üzerine etkileri önemsiz bulunmuştur (P>0,05). Bununla birlikte, kesim sırasında alınan kan örneklerinde Malta ırkından oğlakların plazma LDH düzeyi Saanen ırkı oğlaklarınkinden (P<0,05), plazma CK düzeyi ise Saanen, Gökçeada ve Kıl Keçisi ırkı oğlaklarınkinden (P<0,05) önemli düzeyde yüksek bulunmuştur. Irkın PCV ve total protein düzeyi üzerine etkisi her iki kan alma dönemi için de önemli bulunmuştur. Plazma total protein konsantrasyonu hem besi bölmesinde hem de kesim sırasında yapılan ölçümlerde diğer ırklara oranla Kıl keçisi ırkı oğlaklarda önemli düzeyde düşük bulunmuştur (P<0,01). Araştırma sonucunda kesim öncesi hayvanlara uygulanan prosedürlerin Saanen, Malta, Gökçeada ve Kıl Keçisi oğlaklarında bir stres yanıtına neden olduğu, fakat kesim öncesi yapılan uygulamalara ilişkin stres uyaranlarına verilen kortizol ve glukoz yanıtı üzerine ırkın önemli bir etkisinin olmadığı belirlenmiştir. Diğer yandan, kesim sırasında belirlenen CK ve LDH düzeyleri üzerine ırkın etkisinin önemli olduğu ve bunun da muhtemelen ırklar arasında ki mizaç farklılığından kaynaklandığı sonucuna varılmıştır.

Anahtar Kelimeler: Oğlak, ırk etkisi, kesim sırası stres parametreleri

Keywords

Oğlak, ırk etkisi, kesim sırası stres parametreleri

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