Abstract
Sheeppox and goatpox viruses cause infections in sheep and goats characterized by pyrexia, generalized or local pox lesions, and lymphadenopathy. The sheeppox virus, goatpox virus and lumpy skin disease virus are ordered in the Capripoxvirus genus of Poxviridae family. The aim of this study was to assess the genomic relationships of vaccine strains Bakırköy strain and Kenya0240 strain and of other reference strains. To show the genomic relationships, LD116 gene region coding the viral P32 protein and CD47 gene region from membrane protein genes were chosen. The DNA sequences and as references DNA sequences of the genes of the sheeppox, goatpox and lumpy skin disease viruses obtained from the NCBI Gene Bank were analyzed in DNASTAR gene analysis program using the Clustal W analysis method to determine their differences as to nucleotides and aminoacids. For phylogenetic relationships between the viruses and similarity indices the Neighbor-Joining method was used. The sequence analysis of both gene regions showed that Bakırköy strain belonged to the group where there was only sheeppox virus (Lineage I) and that Kenya0240 strain belonged to the group of the other viruses (Lineage II). Potential vaccine strains protecting not only against sheeppox and goatpox, but also against the lumpy skin disease is significantly important in eradicating the disease.
References
- Babiuk, S., Bowden, T.R., Boyle, D.B., Wallace, D.B., Kitching, R.P., 2008. Capripoxviruses: An emerging worldwide threat to sheep, goats and cattle. Transboundary and Emerging Diseases 55, 263-272.
- Bhanuprakash, V., Hosamani, M., Venkatesan, G., Balamurugan, V., Yogisharadhya, R., Singh, R.K., 2012. Animal poxvirus vaccines: A comprehensive review, 11 (11), 1355-74.
- Bhanuprakash, V., Indrani, B.K., Hosamani, M., Singh, R.K., 2006. The current status of sheep pox disease. Comperative Immunology, Microbiology and Infection Diseases 29 (1), 27
- Bhanuprakash, V., Venkatesan, G., Balamurugan, V., Hosamani, M., Yogisharadhya, R., Chauhan, R.S., Pande, A., Mondal, B., Singh, R.K., 2010. Pox outbreaks in sheep and goats at Makhdoom (Uttar Pradesh), India: Evidence of sheeppox virus infection in goats. Transboundary and Emerging Diseases 57 (5), 375-3
- El-Kenawy, A.A., El-Tholoth, M.S., 2010. Sequence analysis of attachment gene of lumpy skin disease and sheep poxviruses. Virologica Sinica 25 (6), 409-16.
- Gulbahar, M.Y., Davis, W.C., Yuksel, H., Cabalar, M., 2006. Immunohistochemical evaluation of inflammatory infiltrate in the skin and lung of lambs naturally infected with sheeppox virus. Veterinary Pathology 43, 67-75.
- Heine, H.G., Stevens, M.P., Foord, A.J., Boyle, D.B., 19 A capripoxvirus detection PCR and antibody ELISA based on the major antigen P32, the homolog of the vaccinia virus H3L gene. Journal of Immunological Methods 227 (1-2), 187-196. Hosamani, M., Mondal, B., Tembhurne, P.A., Bandyopadhyay, S.K., Singh, R.K., Rasool, T.J., 2004. Differentiation of sheep pox and goat poxviruses by sequence analysis and PCRRFLP of P32 gene. Virus Genes 29, 73-80.
- King, A.M.Q., Adams, M.J., Carstens, E.B., Lefkowitz, E.J., 2012. Virus taxonomy classification and nomenclature of viruses. In: Ninth report of the international committee on taxonomy of viruses. Elsevier Press. Oxford, UK., pp. 291-297.
- Kitching, R.P., 2003. Vaccines for lumpy skin disease, sheep pox and goat pox. Developments in Biological Standardization (Basel) 114, 1611
- Oğuzoğlu, T.C., Alkan, F., Özkul, A., Vural, A.S., Güngör, A.B., Burgu, I., 2006. A sheeppox virus outbreak in central Turkey in 2003: Isolation and identification of capripoxvirus ovis. Veterinary Research Communications 30, 965-9
- Rao, T.V.S., Bandyopadhyay, S.K., 2000. A comprehensive review of goat pox and shepppox and their diagnosis. Animal Health Research Reviews 1 (2), 127-136.
- Tian, H., Chen, Y., Wu, J., Shang, Y., Liu, X., 2010. Serodiagnosis of sheeppox and goatpox using an indirect ELISA based on synthetic peptide targeting for the major antigen P32. Virology Journal 7, 245-249.
- Tulman, E.R., Afonso, C.L., Lu, Z., Zsak, L., Sur, J.H., Sandybaev, N.T., Kerembekova, U.Z., Zaitsev, V.L., Kutish, G.F., Rock, D.L., 2002. The Genomes of Sheeppox and Goatpox Viruses. Journal of Virology 76 (12), 605460
Abstract
Sheeppox ve goatpox virusları, koyun ve keçilerde pyrexia, generalize veya lokal çiçek lezyonları ve
lymphodenopathy ile karakterize bir enfeksiyondur. Sheeppox virusu, goatpox virusu ve lumpy skin disease virusu
Poxviridae ailesindeki Capripoxvirus cinsi içerisinde bulunmaktadır. Bu araştırmada aşı suşu olarak kullanılan
Bakırköy suşu ile Kenya0240 suşu arasında ve diğer referans suşlar arasındaki genomik ilişkilerin incelenmesi
hedeflendi. Bu genomik ilişkilerin ortaya konulmasında virusun P32 proteinini kodlayan LD116 gen bölgesi ve
membran protein genlerinden CD47 gen bölgesi seçildi. DNA dizilimleri ile NCBI Gen bankasından referans gen
olarak seçilen sheppox, goatpox ve lumpy skin diseases viruslarının DNA dizilimleri ClustalW analiz yöntemi
kullanılarak DNASTAR gen analiz programında nüklotid ve aminoasit farklılıkları analiz edildi. Viruslar arasındaki
filogenetik ilişkiler ve benzerlik indeksleri için Neighbor-Joining yöntemi kullanıldı. Her iki gen bölgesinin sekans
analizleri sonucunda Bakırköy suşu sadece koyun çiçek viruslarının bulunduğu grupta (Lineage I) bulunurken,
Kenya0240 suşu diğer virusların da içerisinde bulunduğu grupta (Lineage II) olduğu saptandı. Potansiyel aşı suşlarının
sadece koyun veya keçi çiçeğine değil aynı zamanda sığırların lumpy skin disease virusuna karşı da koruma
sağlayacak nitelikte olması, bu hastalığın eradikasyonu için çok önemlidir.
References
- Babiuk, S., Bowden, T.R., Boyle, D.B., Wallace, D.B., Kitching, R.P., 2008. Capripoxviruses: An emerging worldwide threat to sheep, goats and cattle. Transboundary and Emerging Diseases 55, 263-272.
- Bhanuprakash, V., Hosamani, M., Venkatesan, G., Balamurugan, V., Yogisharadhya, R., Singh, R.K., 2012. Animal poxvirus vaccines: A comprehensive review, 11 (11), 1355-74.
- Bhanuprakash, V., Indrani, B.K., Hosamani, M., Singh, R.K., 2006. The current status of sheep pox disease. Comperative Immunology, Microbiology and Infection Diseases 29 (1), 27
- Bhanuprakash, V., Venkatesan, G., Balamurugan, V., Hosamani, M., Yogisharadhya, R., Chauhan, R.S., Pande, A., Mondal, B., Singh, R.K., 2010. Pox outbreaks in sheep and goats at Makhdoom (Uttar Pradesh), India: Evidence of sheeppox virus infection in goats. Transboundary and Emerging Diseases 57 (5), 375-3
- El-Kenawy, A.A., El-Tholoth, M.S., 2010. Sequence analysis of attachment gene of lumpy skin disease and sheep poxviruses. Virologica Sinica 25 (6), 409-16.
- Gulbahar, M.Y., Davis, W.C., Yuksel, H., Cabalar, M., 2006. Immunohistochemical evaluation of inflammatory infiltrate in the skin and lung of lambs naturally infected with sheeppox virus. Veterinary Pathology 43, 67-75.
- Heine, H.G., Stevens, M.P., Foord, A.J., Boyle, D.B., 19 A capripoxvirus detection PCR and antibody ELISA based on the major antigen P32, the homolog of the vaccinia virus H3L gene. Journal of Immunological Methods 227 (1-2), 187-196. Hosamani, M., Mondal, B., Tembhurne, P.A., Bandyopadhyay, S.K., Singh, R.K., Rasool, T.J., 2004. Differentiation of sheep pox and goat poxviruses by sequence analysis and PCRRFLP of P32 gene. Virus Genes 29, 73-80.
- King, A.M.Q., Adams, M.J., Carstens, E.B., Lefkowitz, E.J., 2012. Virus taxonomy classification and nomenclature of viruses. In: Ninth report of the international committee on taxonomy of viruses. Elsevier Press. Oxford, UK., pp. 291-297.
- Kitching, R.P., 2003. Vaccines for lumpy skin disease, sheep pox and goat pox. Developments in Biological Standardization (Basel) 114, 1611
- Oğuzoğlu, T.C., Alkan, F., Özkul, A., Vural, A.S., Güngör, A.B., Burgu, I., 2006. A sheeppox virus outbreak in central Turkey in 2003: Isolation and identification of capripoxvirus ovis. Veterinary Research Communications 30, 965-9
- Rao, T.V.S., Bandyopadhyay, S.K., 2000. A comprehensive review of goat pox and shepppox and their diagnosis. Animal Health Research Reviews 1 (2), 127-136.
- Tian, H., Chen, Y., Wu, J., Shang, Y., Liu, X., 2010. Serodiagnosis of sheeppox and goatpox using an indirect ELISA based on synthetic peptide targeting for the major antigen P32. Virology Journal 7, 245-249.
- Tulman, E.R., Afonso, C.L., Lu, Z., Zsak, L., Sur, J.H., Sandybaev, N.T., Kerembekova, U.Z., Zaitsev, V.L., Kutish, G.F., Rock, D.L., 2002. The Genomes of Sheeppox and Goatpox Viruses. Journal of Virology 76 (12), 605460
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | January 3, 2014 |
| Published in Issue | Year 2014 Volume: 40 Issue: 1 |