At Rasyonuna Farklı Düzeylerde İlave Edilen Psilyum’un in vitro Fermentasyon Parametreleri ve Metan Salınımına Etkisi

Year 2017, Volume: 43 Issue: 1, 12 - 18, 01.01.2017

Kanber Kara Erol Baytok

https://doi.org/10.16988/iuvfd.255208

Abstract

    Bu
çalışmanın amacı, at rasyonlarına psilyum ilavesinin metan salınımı ve sindirim
parametrelerine etkisinin at dışkısı inokulumu kullanarak in vitro sindirim tekniği ile saptanmasıdır. At rasyonlarına 0
(kontrol grubu), 5, 10, 20 ve 40 g/kg kuru madde (KM) (deneme grupları)
düzeyinde psilyum (Psyllium Husk, Solgar) ilavesinin in vitro toplam gaz ve metan üretimi ile metabolik enerji (ME)
organik madde sindirimi (OMS), amonyak azotu (NH₃-N), kısa zincirli
yağ asitleri (SCFA) ve pH değerlerine etkisi belirlenmiştir. İn vitro sindirim denemesi 100 ml’lik cam
şırıngalar (Model Fortuna, Almanya) içinde 24 saat süresince 39.0±0.2°C’lik
inkübasyonda gerçekleştirilmiştir. Çalışmada, in vitro toplam gaz üretimi kontrol grubuna (181 ml/g KM) göre
deneme gruplarında (130 ml/g KM’e kadar) linear olarak azalmıştır (P<0,001).
Metan üretiminin psilyum ilavesinin artışıyla yaklaşık %35’e kadar azaldığı
saptanmıştır (P≤0,01). At rasyonunun ME, OMS ve SCFA düzeyi psilyum ilavesi ile
olumsuz etkilenmiştir (P<0,01). Sindirim sıvılarının NH₃-N ve pH
düzeyi at rasyonlarına 5, 10, 20 ve 40 g/kg KM psilyum ilavesi ile
değişmemiştir (P>0,05). Sonuç olarak, konstipe atlarda laksatif etkinliğinden
dolayı yaygın olarak kullanılan psilyumun, bir başka olumlu etkisi olarak
atlarda metan salınımını azalttığı ortaya konmuştur. Ancak, metan üretimini
azaltmasına karşın; at rasyonunun in
vitro sindirilebilirliğini olumsuz etkilediğinden dolayı daha düşük
dozlarının in vitro veya in vivo sindirim denemeleriyle
araştırılması ya da sağlıklı atlarda psilyumun uzun süre kullanılmaması
gerektiği düşünülmektedir.

Keywords

Gaz üretimi, in vitro sindirim tekniği, metan, Psilyum

Effect of Different Level of Psyllium Supplementation to Horse Diet on in vitro Fermentation Parameters and Methane Emission

Abstract

    The purpose of this
study was to determine the effect of psyllium addition to horse diets on
methane emissions and digestion parameters by in vitro digestion technique using horse feces as inoculum. The
effect of 0 (control group), 5, 10, 20 and 40 g/kg DM (Dry matter) (treatment
groups) psyllium (Psyllium Husk, Solgar, UK) supplementations to horse diet
were determined on in vitro total gas
and methane production, metabolisable energy (ME), organic matter digestion
(OMD), ammonia nitrogen (NH₃-N), short chain fatty acids (SCFA) and
pH value. In vitro digestibility
technique was performed with using glass syringes of 100 ml volumes (Model Fortuna,
Germany) at 39.0±0.2°C for 24 hour incubation. In the study, in vitro total gas production was
linearly decreased in treatment groups (up to 130 ml/g DM) compared to control
group (181 ml/g DM) (P<0.001). Inclusion of psyllium to horse ration
decreased methane production up to 35% (P≤0.01). The ME, OMD and SCFA levels of
horse diet affected by the psyllium supplementation (P<0.01). Addition of
psyllium at the levels of 5, 10, 20 and 40 g/kg DM did not have an effect on NH₃-N
and pH (P>0.05). Consequently, it was demonstrated that psyllium, which use
commonly in constipated horses because of laxative efficacy, reduced methane
emission as another positive effect in horses. Although psyllium reduced
methane emission, it had adverse effects on in
vitro digestibility of horse ration. However, it was considered that
further investigations are necessary to understand the effects deeply by doing
the in vitro or in vivo digestion
trials with lower doses or psyllium is not suitable to use in healthy horses
for a long time.

Keywords

Gas production, in vitro digestion technique, methane, Psyllium

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