Year 2017, Volume 43 , Issue 1, Pages 1 - 6 2017-01-01

Determination of Ampicillin Resistant Enterococci (Are) Isolated From Canine and Feline Rectal Swabs
Köpek ve Kedi Rektal Svablarından İzole Edilen Ampisilin-Dirençli Enterokokların İncelenmesi

Baran CELIK [1] , Arzu Funda BAGCIGIL [2] , Lora KOENHEMSI [3] , Mehmet Cemal ADIGUZEL [4] , Mehmet Erman OR [5] , Seyyal AK [6]


    Enterococci species, which are normal inhabitants of the gut flora of healthy animals and human, began to be recognized as an important pathogen in both human and veterinary medicine due to the acquired resistance profiles. The aim of the study is to examine the diversity of ampicillin resistance enterococci (ARE) species in cats and dogs, their antimicrobial susceptibility profiles and to determine some of the virulence related genes; ace, gelE, efaA, agg and esp. For this purpose, rectal swabs from companion animals were collected and processed for ampicillin resistant enterococci isolation. One hundred fifty seven swab samples (86 canine and 71 feline) were examined. ARE were isolated from 18 canine and 18 feline samples. All isolates identified as E. faecium by PCR. Antimicrobial susceptibilities of the isolates were determined by disk diffusion method. The isolates were resistant to ampicillin, penicillin, tetracycline (100%), followed by rifampicin and erythromycin (97%), streptomycin (92%), gentamicin (81%), ciprofloxacin (61%), nitrofurantoin (19%). Only two of E. faecium isolates were resistant to vancomycin and one to chloramphenicol. Multidrug resistance (resistance ≥4 antimicrobials) observed in all isolates. Virulence genes ace, agg and esp were not detected in any of the tested isolates. The efaA and gelE genes detection rates were, 13.8% and 11.1% respectively. The ARE isolation rate among pet animals was 22.9%. Screening of antimicrobial resistant enterococci among companion animals would be useful to detect any emerging antimicrobial resistance problem related with public health.

Sağlıklı hayvanlar ve insanların barsak florasının bir parçası olan Enterekok türleri, kazanılmış direnç profilleri nedeniyle hem insan hem de hayvan hekimliğinde önemli birer patojen olarak tanınmaya başlanmıştır. Bu çalışmada, kedi ve köpeklerde ampisiline dirençli enterokok (ARE) türlerinin dağılımını, antimikrobiyal duyarlılık profillerini ve bu izolatların virülans ile ilişkili ace, gelE, efaA, agg ve esp genleri incelendi. Bu amaçla evcil hayvanlardan (86 köpek, 71 kedi) toplanan yüz elli yedi svap örneği ampisilin dirençli enterokok izolasyonu yönünden incelendi. ARE 18 kedi ve 18 köpek örneğinden izole edildi. İzolatların antimikrobiyal duyarlılıkları disk difüzyon metodu ile belirlendi. Bütün izolatlar PCR ile E.faecium olarak saptandı. İzolatlar ampisilin, penisilin ve tetrasiklin (%100), rifampisin ve eritromisin (%97), streptomisin (%92), gentamisin (%81), siprofloksasin (%61) ve nitrofurantoin’e (%19) dirençliydi. Sadece iki izolat vankomisine, bir izolat kloramfenikole dirençliydi. Bütün izolatlarda çoklu-antibiyotik direnci (direnç ≥4 antimikrobiyal) saptandı. Virülens genleri ace, agg ve esp test edilen hiçbir izolatta saptanmadı. efaA ve gelE genlerinin saptanma oranları sırasıyla %13,8 ve %11,1 di. Pet hayvanlarında ARE izolasyon oranı %22,9 olarak saptandı. Evcil hayvanlar arasında antimikrobiyal dirençli Enterokokların taranması halk sağlığı ile ilişkilidir. Ayrıca hayvan ve insanlarda antimikrobiyal direnç sorununu tespit etmek ve zamanında önlem alabilmek konusunda yapılan çalışmanın aydınlatıcı olabileceği düşünülmektedir.

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Subjects Health Care Sciences and Services
Journal Section Research Article
Authors

Author: Baran CELIK
Institution: İstanbul Üniveristesi

Author: Arzu Funda BAGCIGIL
Institution: İstanbul Üniveristesi

Author: Lora KOENHEMSI
Institution: İstanbul Üniveristesi

Author: Mehmet Cemal ADIGUZEL
Institution: İstanbul Üniveristesi

Author: Mehmet Erman OR
Institution: İstanbul Üniveristesi

Author: Seyyal AK
Institution: İstanbul Üniveristesi

Dates

Publication Date : January 1, 2017

Bibtex @research article { iuvfd265324, journal = {İstanbul Üniversitesi Veteriner Fakültesi Dergisi}, issn = {0250-2836}, eissn = {2148-8320}, address = {}, publisher = {İstanbul University-Cerrahpaşa}, year = {2017}, volume = {43}, pages = {1 - 6}, doi = {10.16988/iuvfd.265324}, title = {Köpek ve Kedi Rektal Svablarından İzole Edilen Ampisilin-Dirençli Enterokokların İncelenmesi}, key = {cite}, author = {CELIK, Baran and BAGCIGIL, Arzu Funda and KOENHEMSI, Lora and ADIGUZEL, Mehmet Cemal and OR, Mehmet Erman and AK, Seyyal} }
APA CELIK, B , BAGCIGIL, A , KOENHEMSI, L , ADIGUZEL, M , OR, M , AK, S . (2017). Köpek ve Kedi Rektal Svablarından İzole Edilen Ampisilin-Dirençli Enterokokların İncelenmesi. İstanbul Üniversitesi Veteriner Fakültesi Dergisi , 43 (1) , 1-6 . DOI: 10.16988/iuvfd.265324
MLA CELIK, B , BAGCIGIL, A , KOENHEMSI, L , ADIGUZEL, M , OR, M , AK, S . "Köpek ve Kedi Rektal Svablarından İzole Edilen Ampisilin-Dirençli Enterokokların İncelenmesi". İstanbul Üniversitesi Veteriner Fakültesi Dergisi 43 (2017 ): 1-6 <https://dergipark.org.tr/en/pub/iuvfd/issue/23931/265324>
Chicago CELIK, B , BAGCIGIL, A , KOENHEMSI, L , ADIGUZEL, M , OR, M , AK, S . "Köpek ve Kedi Rektal Svablarından İzole Edilen Ampisilin-Dirençli Enterokokların İncelenmesi". İstanbul Üniversitesi Veteriner Fakültesi Dergisi 43 (2017 ): 1-6
RIS TY - JOUR T1 - Köpek ve Kedi Rektal Svablarından İzole Edilen Ampisilin-Dirençli Enterokokların İncelenmesi AU - Baran CELIK , Arzu Funda BAGCIGIL , Lora KOENHEMSI , Mehmet Cemal ADIGUZEL , Mehmet Erman OR , Seyyal AK Y1 - 2017 PY - 2017 N1 - doi: 10.16988/iuvfd.265324 DO - 10.16988/iuvfd.265324 T2 - İstanbul Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 1 EP - 6 VL - 43 IS - 1 SN - 0250-2836-2148-8320 M3 - doi: 10.16988/iuvfd.265324 UR - https://doi.org/10.16988/iuvfd.265324 Y2 - 2015 ER -
EndNote %0 İstanbul Üniversitesi Veteriner Fakültesi Dergisi Köpek ve Kedi Rektal Svablarından İzole Edilen Ampisilin-Dirençli Enterokokların İncelenmesi %A Baran CELIK , Arzu Funda BAGCIGIL , Lora KOENHEMSI , Mehmet Cemal ADIGUZEL , Mehmet Erman OR , Seyyal AK %T Köpek ve Kedi Rektal Svablarından İzole Edilen Ampisilin-Dirençli Enterokokların İncelenmesi %D 2017 %J İstanbul Üniversitesi Veteriner Fakültesi Dergisi %P 0250-2836-2148-8320 %V 43 %N 1 %R doi: 10.16988/iuvfd.265324 %U 10.16988/iuvfd.265324
ISNAD CELIK, Baran , BAGCIGIL, Arzu Funda , KOENHEMSI, Lora , ADIGUZEL, Mehmet Cemal , OR, Mehmet Erman , AK, Seyyal . "Köpek ve Kedi Rektal Svablarından İzole Edilen Ampisilin-Dirençli Enterokokların İncelenmesi". İstanbul Üniversitesi Veteriner Fakültesi Dergisi 43 / 1 (January 2017): 1-6 . https://doi.org/10.16988/iuvfd.265324
AMA CELIK B , BAGCIGIL A , KOENHEMSI L , ADIGUZEL M , OR M , AK S . Köpek ve Kedi Rektal Svablarından İzole Edilen Ampisilin-Dirençli Enterokokların İncelenmesi. iuvfd. 2017; 43(1): 1-6.
Vancouver CELIK B , BAGCIGIL A , KOENHEMSI L , ADIGUZEL M , OR M , AK S . Köpek ve Kedi Rektal Svablarından İzole Edilen Ampisilin-Dirençli Enterokokların İncelenmesi. İstanbul Üniversitesi Veteriner Fakültesi Dergisi. 2017; 43(1): 6-1.