Determination of Ampicillin Resistant Enterococci (Are) Isolated From Canine and Feline Rectal Swabs

Year 2017, Volume: 43 Issue: 1, 1 - 6, 01.01.2017

Baran Celık , Arzu Funda Bagcıgıl , Lora Koenhemsı , Mehmet Cemal Adıguzel Mehmet Erman Or , Seyyal Ak

https://doi.org/10.16988/iuvfd.265324

Abstract

    Enterococci species,
which are normal inhabitants of the gut flora of healthy animals and human,
began to be recognized as an important pathogen in both human and veterinary
medicine due to the acquired resistance profiles. The aim of the study is to
examine the diversity of ampicillin resistance enterococci (ARE) species in
cats and dogs, their antimicrobial susceptibility profiles and to determine
some of the virulence related genes; ace,
gelE, efaA, agg and esp. For this
purpose, rectal swabs from companion animals were collected and processed for
ampicillin resistant enterococci isolation. One hundred fifty seven swab
samples (86 canine and 71 feline) were examined. ARE were isolated from 18
canine and 18 feline samples. All isolates identified as E. faecium by PCR. Antimicrobial susceptibilities of the isolates
were determined by disk diffusion method. The isolates were resistant to
ampicillin, penicillin, tetracycline (100%), followed by rifampicin and erythromycin
(97%), streptomycin (92%), gentamicin (81%), ciprofloxacin (61%),
nitrofurantoin (19%). Only two of E. faecium
isolates were resistant to vancomycin and one to chloramphenicol. Multidrug
resistance (resistance ≥4 antimicrobials) observed in all isolates. Virulence
genes ace, agg and esp were not detected in any of the
tested isolates. The efaA and gelE genes detection rates were, 13.8%
and 11.1% respectively. The ARE isolation rate among pet animals was 22.9%.
Screening of antimicrobial resistant enterococci among companion animals would
be useful to detect any emerging antimicrobial resistance problem related with
public health.

Keywords

Antimicrobial resistance, cat, dog, PCR, ampicillin resistant Enterococci

Köpek ve Kedi Rektal Svablarından İzole Edilen Ampisilin-Dirençli Enterokokların İncelenmesi

Abstract

Sağlıklı
hayvanlar ve insanların barsak florasının bir parçası olan Enterekok türleri,
kazanılmış direnç profilleri nedeniyle hem insan hem de hayvan hekimliğinde önemli
birer patojen olarak tanınmaya başlanmıştır. Bu çalışmada, kedi ve köpeklerde
ampisiline dirençli enterokok (ARE) türlerinin dağılımını, antimikrobiyal
duyarlılık profillerini ve bu izolatların virülans ile ilişkili ace, gelE, efaA, agg ve esp genleri incelendi. Bu amaçla evcil
hayvanlardan (86 köpek, 71 kedi) toplanan yüz elli yedi svap örneği ampisilin
dirençli enterokok izolasyonu yönünden incelendi. ARE 18 kedi ve 18 köpek
örneğinden izole edildi. İzolatların antimikrobiyal duyarlılıkları disk difüzyon
metodu ile belirlendi. Bütün izolatlar PCR ile E.faecium olarak
saptandı. İzolatlar ampisilin, penisilin ve tetrasiklin (%100), rifampisin ve
eritromisin (%97), streptomisin (%92), gentamisin (%81), siprofloksasin (%61)
ve nitrofurantoin’e (%19) dirençliydi. Sadece iki izolat vankomisine, bir
izolat kloramfenikole dirençliydi. Bütün izolatlarda çoklu-antibiyotik direnci
(direnç ≥4 antimikrobiyal) saptandı. Virülens genleri ace, agg ve esp test
edilen hiçbir izolatta saptanmadı. efaA
ve gelE genlerinin saptanma oranları
sırasıyla %13,8 ve %11,1 di. Pet hayvanlarında ARE izolasyon oranı %22,9 olarak
saptandı. Evcil hayvanlar arasında antimikrobiyal dirençli Enterokokların
taranması halk sağlığı ile ilişkilidir. Ayrıca hayvan ve insanlarda
antimikrobiyal direnç sorununu tespit etmek ve zamanında önlem alabilmek
konusunda yapılan çalışmanın aydınlatıcı olabileceği düşünülmektedir.

Keywords

Antimikrobiyal direnç, kedi, köpek, PCR, ampisilin dirençli Enterokok

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