Occurrence of Malassezia pachydermatis in Dogs with Otitis Externa

Year 2016, Volume: 42 Issue: 2, 117 - 121, 04.07.2016

Kemal Metiner , Baran Çelik , Beren Başaran Kahraman , Belgi Diren Sığırcı , Zehra Seda Mavili Seyyal Ak

https://doi.org/10.16988/iuvfd.2016.93199

Abstract

Swab samples taken from a total of 100 dogs which were suspected about otitis externa symptoms such as ear discharge, skin rash (exanthema), flaking off, inflammation, rash and stink were mycologically examined. The swabs were inoculated onto Sabouraud Dextrose Agar (SDA) containing 0.05% chloramphenicol and 0.05% cycloheximide, and were incubated at 37 °C for 7 days. After the incubation, isolates were evaluated by morphological, physiological and biochemical characteristics. Thirty six out of these isolates were identified as Malessezia pachydermatis, six as Candida spp., and two of them were identified as Aspergillus spp. M. pachydermatis was isolated from 15 of 33 (42.4%) one to three years old, 10 of 30 (33.3%) four to six years old, 8 of 19 (42.1%) seven to ten years old, 1 of 8 (12.5%) ten to twelve years old and 2 of 9 (22.2%) older than twelve years old dogs. There has been no isolation occured under one year old dog. The agent was isolated 17 of 46 (36.9%) females, while 19 of 54 (35.1%) males. Crossbred animals and the terriers have the highest occurrence rates (25 and 22.2% respectively) if the percentage of M. pachydermatis isolation from a breed verses all affected dogs was evaluated. Though, German Shepherds have the highest occurrence rate (57.1%) when evaluated within the breeds. Differences according to age, gender and breed characteristics were not statistically significant (P>0.05).

Otitis Eksternalı Köpeklerde Malassezia pachydermatis’in Varlığı

Abstract

Kulak akıntısı, deride kızarıklık (egzantem), kabuklanma, yangı, kızarıklık ve kötü koku gibi semptomlara sahip olan otitis eksterna şüpheli toplam 100 köpekten kulak svab örneği mikolojik yönden incelendi. Svablar, 0.05% kloramfenikol ve 0.05% sikloheksimid içeren Sabouraud Dextrose Agara (SDA) ekildi ve 37 °C de 7 gün inkübe edildi. İnkübasyonun ardından, izolatlar morfolojik, fizyolojik ve biyokimyasal özelliklerine göre değerlendirildi. İzolatların 36 adeti Malassezia pachydermatis, altısı Candida spp. ve iki adeti Aspergillus spp. olarak identifiye edildi. M.pachydermatis; bir üç yaş arası 33 köpeğin on beşinde (%42,4), dört altı yaş arası 30 köpeğin onundan (%33,3), yedi on yaş arası 19 köpeğin sekizinden (%42,1), on 12 yaş arası sekiz köpeğin birinden (%12,5) ve 12 yaşından büyük dokuz köpeğin ikisinden (%22,2) izole edildi. Bir yaş altında olan tek köpekte üreme olmadı. Etken, 46 dişi köpeğin 17’sinden (%36,9) ve 54 erkek köpeğin 19’undan (%35,1) izole edildi. Karışık köpek ırkları ve terrierler (%25 ve 22,2), M. pachydermatis izolasyonu incelenen bütün hayvanlar ile karşılaştırılırsa en yüksek orana sahipti. Bununla birlikte, ırklar içinde değerlendirildiğinde German Shepherd köpeklerin (%57,1) en yüksek izolasyon oranına sahip olduğu belirlendi. Yaş, cinsiyet ve ırk özelliklerine göre farklılıklar istatistiki olarak önemsiz bulundu (P>0,05). Sonuç olarak, köpeklerden yüksek oranda M. pachydermatis izolasyonunun otitis eksterna infeksiyonlarında önemli role sahip olduğunu düşündürmektedir.

Keywords

Köpek, otitis eksterna, Malassezia pachydermatis, izolasyon

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