AKIM SİTOMETRİ VE SU ÜRÜNLERİ UYGULAMALARI

Abstract

Akım sitometri (AS), 0.2–150 µm büyüklüğündeki partiküllerin genellikle de hücrelerin tek tek, bir ışık demetinin önünden, bir sıvı içinde geçerken, anlık olarak ölçümlerini yapan daha sonra da çoklu fiziksel özelliklerini analiz eden bir teknolojidir. Akım sitometri, hücre biyolojisini birçok açıdan araştırmak ve istenilen hücreleri izole etmek için kullanılır. Akım sitometri çok sayıda tek hücrenin birçok karakteristiklerini hızlı bir şekilde ölçtüğünden hücre tabanlı analizde altın standart olarak kabul edilmiştir. Diğer tekniklerle karşılaştırıldığında, Akım sitometri’nin hızlı veri eldesini ve çok parametreli analizi kolaylaştırması popülaritesinin artmasına ve uygulama alanlarının genişlemesine yol açmıştır. Bu derlemede, Akım sitometri’nin su ürünleri alanındaki mevcut durumu ve uygulamaları hakkında özet bir şekilde bilgi verilmiştir.

Keywords

• Akım Sitometri,Balıklarda fagositik aktivite,Hücre taban-lı analiz,Akuatik toksikoloji,Plankton,Hemosit

.FLOW CYTOMETRY AND ITS APPLICATIONS IN AQUTIC SCIENCES

Abstract

Flow cytometry (FCM) is a technology that simultaneously measures and then analyzes multiple physical characteristics of 0.2–150 µm sized particles, usually cells, as they flow one by one in a fluid stream through a beam of light. Flow cytometry is used for investigating many aspects of cell biology and for isolating the cells desired. Since Flow cytometry measures the multiple characteristics of large numbers of individual cells rapidly, it has been accepted as the gold Standard in cell based analysis. Since Flow cytometry facilitates rapid data acquisition and eases multiparameter analysis, leading to increased popularity and widespread applications as compared to other analyzing techniques. In this review, the current status and utilization of Flow cytometry in aquatic sciences are briefly presented.

Keywords

• Flow Cytometry,Phagositic activiy of fish,Cell-based analysis,Aquatic toxicology,Plankton,Haemocyte

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