DETERMINATION OF SOME HEMATOLOGICAL PARAMETERS AND NON-SPECIFIC IMMUNE RESPONSES IN Garra rufa (HECKEL, 1843) LIVING IN KANGAL (SİVAS) BALIKLI ÇERMİK THERMAL HOT SPRING AND TOPARDIÇ STREAM (SİVAS)

Year 2017, , 108 - 115, 18.06.2017

Selçuk Duman , Aysel Şahan

https://doi.org/10.3153/JAEFR17014

Abstract

In this study, Garra rufa Heckel,
1843 (popularly known as doctor
fishes), which is a fatty fish species living in Kangal
Balıklı Çermik Thermal Hot Springs and Topardıç Stream mixing with these hot
spring waters, was investigated. Hematological and non-specific immune parameters
were investigated by comparing each environment in terms of seasons and each
season in terms of environments. The amount of erythrocyte (RBC) and leukocyte (WBC) in the stream was
found to be increased in summer and decreased in winter. On the other hand, no
seasonal differences were observed in the pools due to the thermal water. Hb,
Hct, monocyte, neutrophil, phagocytic activity values were found to be low in
the winters and high in the summers in the stream whereas no changes were
observed in the pools. No difference was found between seasons and environments
in terms of the amount of eosinophil cells. Lymphocyte and MCV levels in the
stream were found to be increased in winter and decreased in summer. No change
was observed in the pools. Seasonal and environmental no difference was
observed in MCH values of both pools and the stream, whereas MCHC values in the
stream were found to be high in summer and low in winter. In the research,
differences in winter and summer seasons and effects of different environments
in these values were investigated.

Keywords

Garra rufa, Kangal Balıklı Çermik Thermal Hot Springs, Hematology, Non-Specific Immunity

References

• Adeyemo, O.K., Okwilagwe, O.O. & Ajani, F. (2009). Comparative assessment of sodium edta and heparin as anticoagulants for the evaluation of haematological parameters in cultured and feral African cathfish (Clarias gariepinus). Brazilian Journal of Aquatic Science and Technology, 13(1), 19-24. Akbulut, K., Zengin, U., Gönek, Ü. & Bakır, M. (2008). Balıklarda yaş tayini yöntemleri veönemi. Erzincan Üniversitesi Aquaclub Su Ürünleri Araştırma ve Geliştirme Bilim Kulübü Kemaliye 5. Geleneksel Su Ürünleri Bilimsel ve Kültürel Platformu, Kemaliye, Erzincan. Akmirza, A. & Tepecik, R.E. (2007). Seasonal variation in some haematological parameters in naturally infected and uninfected roach (Rutilus rutilus) with Cryptobia tincae. Journal of Applied Biological Sciences, 1(3), 61-65. Aras, M., Bayır, A., Sirkecioğlu, A.N., Polat, H. & Bayır, M. (2008). Seasonal variations in serum lipids, lipoproteins and some haematological parameters of chub (Leuciscus cephalus). Italian Journal of Animal Science, 7, 439-448. Arnaudova, D., Arnaudova, A. & Tomova, E. (2008). Selected hematological indices of freshwater fish from studen kladenetsh reservoir. Bulgarian Journal of Agricultural Science, 14(2), 244-250. Arnold, J.E. (2005). Hematology of the sandbar shark, Carcharhinus plumbeus standardization of complete blood count techniques for elasmobranchs. Veterinary Clinical Pathology, 34, 115-123. Atamanalp, M. & Yanık, T. (2003). Alterations in hematological parameters of rainbow trout (Oncorhynchus mykiss) exposed to mancozeb. Turkish Journal of Veterinary and Animal Sciences, 27, 1213-1217. Blaxhall, P.C. & Daisley, K.W. (1973). Routine haematological methods for use with fish blood. Journal of Fish Biology, 5(6), 771-781. Cengizler, İ. & Şahan, A. (2000). Seyhan Baraj Gölü ve Seyhan Nehrin de yaşayan aynalısazan (Cyprinus carpio, Linnaeus, 1758)’ larda bazı kan parametrelerinin belirlenmesi. Turkish Journal of Veterinary and Animal Sciences, 24, 205-214. Clauss, T.M., Alistair, D.M. & Arnold, J.E. (2008). Hematologic disorders of fish. Veterinary Clinics of North America: Exotic Animal Practice, 11(3), 445-462. Clarence, R. & Hickey, J.R. (1982). Comparative hematology of wild and captive cunners. Transactions of the American Fisheries Society, 111, 242-249. Collazos, M.E., Barriga, C. & Ortega, E. (1995). Seasonal variations in the immune system of the cyprinid Tinca tinca phagocytic function. Comparative Immunology, Microbiology and Infectious Diseases, 18(2), 105-113. Demirsoy, A. (1993). Yaşamın Temel Kuralları (Omurgalılar/Anamniyota). Cilt-III/Kısım-I, 38, 387, Hacettepe Üniversitesi Yayınları, Ankara. Dias, M.T., Affonso, E.G., Oliveria, S.R., Marcon, J.L. & Egami, M.I. (2008). Comparative study on hematological parameters of farmed matrinxa, Brycon amazonicus spix and agassiz, 1829 (Characidae: Bryconinae) with others bryconinae species. Acta-Amozanica, 38(4), 799-806. Duman, S. & Şahan, A. (2014). Kangal (Sivas) Balıklı Çermik Termal Kaplıcası İle Topardıç Deresi'nde (Sivas) Yaşayan Benekli Sazan Cyprinion macrostomus (Heckel, 1843) de Bazı Hematolojik Parametreler ve Non-Spesifik İmmün Yanıtın Belirlenmesi. Yunus Araştırma Bülteni, 4, 21-28. Dorafshan, S., Kalbassi, M.R., Pourkazemi, M., Amiri, B.M. & Karimi, S.S. (2008). Effects of triploidy on the caspian salmon Salmo trutta caspius haematology. Fish Physiology and Biochemistry, 34(3), 195-200. Ginneken, V.V., Durif, C., Balm, S.P., Boot, R., Verstegen, M.W.A., Antonissen, E. & Thillart, G.V.D. (2007). Silvering of european ell (Anguilla anguilla L.) seasonal changes of morphological and metabolic parameters. Animal Biology, 57(1), 63-77. Gözükara, S.A. & Çavaş, T. (2004). A karyological analysis of Garra rufa (Heckel, 1843) (Pisces, Cyprinidae) from the eastern Mediterranean River Basin in Turkey. Turkish Journal of Veterinary and Animal Sciences, 28, 497-500. Grigg, G.C. (1969). Temperature induced changes in the oxygen equilibrium curve of the blood of the brown bullhead, Ictalurus nebulosus. Comparative Biochemistry and Physiology, 28(3), 1203-1223. Hayran, M. & Özdemir, O. (1995). Bilgisayar İstatistik ve Tıp. Hekimler Yayın Birliği, Ankara. Imanpoor, M.R., Bagheri, T. & Hedayati, S.A.A. (2010). The anesthetic effects of clove essence in persian sturgeon, Acipenser persicus. World Journal of Fish and Marine Sciences, 2(1) 29-36. Jeney, G., Galeotti, M., Volpatti, D., Jeney, Z. & Anderson, D.P. (1997). Prevention of stress in rainbow trout (Oncorhynchus mykiss) fed diets containing different doses of glucan. Aquaculture, 154, 1-15. Kocabatmaz, M. & Ekingen, G. (1984). Değişik Tür Balıklarda Kan Örneği Alınması ve Hematolojik Metotların Standardizasyonu. Doğa Bilim Dergisi, 8, 149-159. Konuk, T. (1981). Pratik Fizyoloji Ders Kitabı, Ankara Üniversitesi Veteriner Fakültesi Yayınları. Ankara Üniversitesi Basımevi. Ankara. Kortet, R. & Vainikka, A. (2008). Seasonality of innate immunity; evolutionary aspects and latest updates. In M. Durand & C.V. Morel (Eds), In New Research on Innate Immunity (pp. 13-45). NY: Nova Science Publishers. ISBN 978-1-60456-549-2. Leard, A.T., Wanger, B.A., Camp, K.L., Wise, D.J. & Gao, X.D. (1998). Seasonal values of selected blood parameters of farm-raised channel catfish (Ictalurus punctatus) in the Mississippi Delta. Journal of Veterinary Diagnostic Investigation, 10, 344-349. Morvan, C.L., Troutaud, D. & Deschaux, P. (1998). Differential effects of temperature on specific and nonspecific immune defences in fish. The Journal Experimental Biology, 201, 165-168. Nanba, K., Kakuta, I., Uematsu, K. & Murachi, S. (1987). Annual changes in the osmolarity and inorganic ion level ratios between urine and plasma in carp Cyprinus carpio. Nippon Suisan Gakkaishi: Bulletin of the Japanese Society of Scientific Fisheries, 53 (6), 913-918. Örün, İ., Dörücü, M. & Yazlak, H. (2003). Hematological parameters of three cyprinid fish species from Karakaya Dam Lake, Turkey. Online Journal of Biological Sciences, 3(3), 320-328. Özer, Z., Akpınar, M.A., Akçay, M., Erdem, Ü., Güler, R., Yanıkoğlu, A., Ergenoğlu, B., Dere, Ş. & Savaşçı, Ş. (1987). Kangal Balıklı Kaplıcanın (Sivas) bazı kimyasal ve biyolojik özelliklerinin araştırılması. Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi, 5, 1-34. Rambhaskar, B. & Rao, S.K. (2006). Comparative haematology of ten species of marine fish from Visakhapatnam Coast. Journal of Fish Biology, 30(1), 59-66. Siwicki, A.K., Anderson, D.P. & Rumsey, G.L. (1994). Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. Veterinary Immunology and Immunopathalogy, 41, 125-139. Swain, P., Dash, S., Sahoo, P.K., Gupta, S.D., Meher, P.K. & Sarangi, N. (2007). Nonspecific immune parameters of brood indian major carp Labeo rohita and their seasonal variations. Fish & Shellfish Immunology, 22(1)2, 38-43. Şahan, A. (2000). Seyhan Nehri (Adana kent içi bölgesi)’nde yaşayan bazı Cyprinid’lerde hematolojik araştırmalar. PhD Thesis Çukurova University. Adana. Şahan, A. & Cengizler, İ. (2002). Seyhan Nehri (Adana kent içi bölgesi)’ nde yaşayan benekli siraz (Capoeta barroisi Lorted, 1894) ve kızılgöz (Rutilus rutilus, Linnaeus, 1758)’ de bazı hematolojik parametrelerin belirlenmesi. Turkish Journal of Veterinary and Animal Sciences, 26, 849-858. Şahan, A. & Duman, S. (2010). Influence of -1,3/1,6 glucan applications on some non-specific cellular immune response and hematologic parameters of healthy nile tilapia (Oreochromis niloticus L., 1758). Turkish Journal of Veterinary and Animal Sciences, 34(1), 75-81. Tanyer, G. (1985). Hematoloji ve Lobaratuvar Ders Kitabı, Ayyıldız A.Ş., Ankara. Timur, M., Çolak, A. & Marufi, M. (1983). Balıklı Kaplıcadaki (Sivas) balık türlerinin tanımı ve deri hastalıkları tedavisindeki etkisinin araştırılması. Ankara Üniversitesi Veterinerlik Fakültesi Dergisi, 30(2), 276-282. Yılayaz, Ö. & Bitmiş, K. (2002). Keban Baraj Gölü’nde yaşayan Barbus rajanorum mystaceus (Heckel, 1843)’ da kan parametrelerinin incelenmesi. Gazi Üniversitesi Gazi Eğitim Fakültesi Dergisi, 22(2), 11-21.