The Effect of Whey on the Immune Parameters of Rainbow Trout (Oncorhynchus mykiss)

Remziye Eda Yardımcı; Emre Turgay; Sevdan Yılmaz; Can Sarvan; Süheyla Karataş Steınum

doi:10.35229/jaes.1018996

TR EN

Peynir Altı Suyunun (PAS) Gökkuşağı Alabalığı (Oncorhynchus mykiss) Bağışıklık Parametreleri Üzerine Etkisi

Öz

Bu çalışmanın amacı kültürü yapılan gökkuşağı alabalıklarının (Oncorhynchus mykiss) bağışıklık sistemi üzerine peynir altı suyunun (PAS) etkisinin incelenmesidir. Süt ve süt ürünleri endüstrisi atığı olan PAS’ın akuakültür endüstrisinde balık bağışıklık sistemi destekleyicisi ve balık yemi protein kaynağı olarak kullanılması hedeflenmiştir. Ortalama ağırlığı 70,28 ± 1,50 g olan gökkuşağı alabalığı bireyleri 10 kg/m3 stok yoğunluğunda olacak şekilde 6 adet tanka yerleştirilmiştir. Kontrol grubu yemlerinde protein kaynağı olarak balık unu kullanılırken deney grubu yemlerinde ise balık unu yerine PAS kullanılmıştır. Balıklar izokalorik (21 kj/g) ve izonitrojenik (%41 protein) olarak hazırlanan bu pelet yemlerle 3 hafta boyunca beslenmiştir. Haftada birer kez olmak üzere balıklardan kan örnekleri alınmış; hematokrit ve hemoglobin değerleri tam otomatik hematoloji analizatörü ile belirlenmiş, eritrosit ve lökosit sayıları ise standart hemositometri yöntemleri kullanılarak yapılmıştır. Bunlara ek olarak oksidatif radikal üretimi ve kompleman 3 konsantrasyonu da kantitatif olarak belirlenmiştir. Sonuçlar tek yönlü varyans analizi (one-way ANOVA) ile değerlendirilmiştir. Sonuç olarak kontrol grubu ile deney grubu arasında istatistiki açıdan herhangi bir farklılık olmadığı görülmüştür (p>0,05). Bununla birlikte, kontrol ve deney grubunda başlangıçta eşit olarak ölçülen C3 kompleman konsantrasyonunun, deney grubunda ikinci haftada kontrol grubuna göre istatistiksel açıdan önemli (p<0,05) bir artış gösterdiği tespit edilmiştir. Bu çalışma, ülkemizde balık yemlerinde PAS kullanılmasının balıkların bağışıklık sistemi üzerine etkisinin araştırıldığı ilk çalışmadır.

Anahtar Kelimeler

Diyet , Gökkuşağı alabalığı , PAS , Bağışıklık sistemi , C3 kompleman

The Effect of Whey on the Immune Parameters of Rainbow Trout (Oncorhynchus mykiss)

Abstract

The aim of this study was to investigate the effect of whey on the immune system of cultured rainbow trout (Oncorhynchus mykiss). It was targeted to use whey, which is a by-product of the dairy industry, in the aquaculture industry as a promoter of fish immune system and as a source of fish feed protein. Rainbow trout individuals with an average weight of 70.28 ± 1.50 g were placed in 6 tanks with a stock density of 10 kg/m3. While fish meal (52%) was used as a protein source in the control group feeds, in the experimental group feeds whey powder (48%) was used. The fish were fed these pellet feeds that were isocaloric (21 kj / g) and isonitrogenous (41% protein) for 3 weeks. Blood samples were collected once a week and while hematocrit and hemoglobin values were determined using a fully automated hematology analyzer, standard hemocytometry methods were used in erythrocyte and leukocyte counts. In addition, free radical production and complement 3 concentrations were determined quantitatively. According to the one-way ANOVA analysis, the control group and the experimental group were statistically similar (p> 0,05). However, it was found that C3 complement concentration, which was measured equally at the beginning in the control and experimental groups, showed a statistically significant increase (p <0.05) in the experimental group compared to the control group in the second week. This is the first study in Turkey investigating the effect of using whey in fish feeds on the immune system of fish.

Keywords

Diet , rainbow trout , WHEY , Immune system , C3 complement

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Remziye Eda Yardımcı ^*
0000-0001-7737-8739
Türkiye

Emre Turgay
0000-0001-9964-3919
Türkiye

Sevdan Yılmaz
0000-0002-4809-5809
Türkiye

Can Sarvan This is me
Türkiye

Süheyla Karataş Steınum
0000-0003-2006-7854
Türkiye

Publication Date

March 31, 2022

Submission Date

November 4, 2021

Acceptance Date

January 24, 2022

Published in Issue

Year 2022 Volume: 7 Number: 1

DOI

https://doi.org/10.35229/jaes.1018996

IZ

https://izlik.org/JA83MS57ER

APA

Yardımcı, R. E., Turgay, E., Yılmaz, S., Sarvan, C., & Karataş Steınum, S. (2022). The Effect of Whey on the Immune Parameters of Rainbow Trout (Oncorhynchus mykiss). Journal of Anatolian Environmental and Animal Sciences, 7(1), 15-20. https://doi.org/10.35229/jaes.1018996

EBSCOHost Scilit CABI

JAES/AAS-Journal of Anatolian Environmental and Animal Sciences/Anatolian Academic Sciences&Anadolu Çevre ve Hayvancılık Dergisi/Anadolu Akademik Bilimler-AÇEH/AAS

Peynir Altı Suyunun (PAS) Gökkuşağı Alabalığı (Oncorhynchus mykiss) Bağışıklık Parametreleri Üzerine Etkisi

Öz

Anahtar Kelimeler

The Effect of Whey on the Immune Parameters of Rainbow Trout (Oncorhynchus mykiss)

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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