The Determination of Antibiotic Resistance and Biofilm Properties in Pseudomonas aeruginosa Isolates from Raw Milk Samples
Abstract
Abstract: In this study, it was aimed to investigate the antibiotic resistance and biofilm properties of Pseudomonas aeruginosa isolates isolated from raw milks. A total number of 300 raw milk samples were collected from several dairy plants and vendors in the provinces of Aydın (n=100), İzmir (n=100) and Muğla (n=100) in Turkey. The conventional methods were used for the isolation of suspected Pseudomonas spp. from raw milk samples. A total of 63 suspected Pseudomonas spp. were isolated and these isolates were identified as being P.aeruginosa by PCR. According to PCR results, 24 isolates were identified as P.aeruginosa. The antibiotic resistances of P. aeruginosa against 8 antibiotics were determined by Kirby-Bauer Disc Diffusion method. The antibiotic resistance rates of the isolates among enrofloxacine, ceftriaxone, ciprofloxacin, meropenem, colistin, gentamycin, and azithromycin were found as 20.84%, 75.0%, 4.16%, 37.33%, 8.34%, 12.5 % and 91.66%, respectively. When examined according to multiple antibiotic resistances, it was determined that 11 isolates (45.83%) were resistant to more than 3 antibiotic groups and were evaluated as multi-resistant. The biofilm formations of the isolates were investigated in vitro with Congo Red Agar Method. The biofilm formation ratios were determined as 37.50% with CRA method. The biofilm formation and multiple antibiotic resistance rates were found as high in the raw milk isolates of P. aeruginosa. The widespread and advanced studies thought to be performed for the lightening of this situation and biofilm related antibiotic resistances.
Keywords
antibiotic resistance , biofilm , Pseudomonas aeruginosa , raw milk.
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