In Vitro Biological Activities Of Ranunculus gracilis Clarke Rhizome

Yıl 2022, , 115 - 121, 30.06.2022

Elif Çil , Ceren Börçek Kasurka , Melek Çol Ayvaz

https://doi.org/10.35229/jaes.1025725

Öz

R. gracilis rhizomes were picked from Yenice Forests Karabük province in Turkey. Ethanol was chosen for extraction solvent. Disc diffusion method including filamentous and non-filamentous Gram-positive bacteria, Gram-negative bacteria, and yeast strains was used to evaluate the antimicrobial activity of the extract. DPPH free radical scavenging activity and ferric reducing antioxidant power analysis were performed to determine antioxidant activity. The Folin-Ciocalteu method for determining the total phenolic amount and the AlCl3 method for the total flavonoid content of the extract was chosen. Mean diameters of inhibition zones (IZD) of the bacteria were found in the range of 8.2 mm to 24.45 mm. This value was measured as 17.82 mm and 18.69 mm for yeasts. The total antioxidant activity value of the extract was calculated as 7.08 mg AAE/g extract. The IC50 value was found as 9.097 mg/mL for DPPH free radical scavenging activity. The FRAP value indicated that the reducing power of 1 gram of sample was equivalent to 4.66 μmol of Trolox. The total phenolic content of ethanol extract of R. gracilis rhizomes was determined as 0.414 mg GAE/g, while the flavonoid content was calculated as 0.68 mgQE/g. The analyzed ethanolic extract of R. gracilis rhizomes demonstrated that the biological activity level could be considered significant according to the obtained results.

Anahtar Kelimeler

Antibacterial effect, Antifungal activity, pathogenic actinomycetes, antioxidant.

Teşekkür

The authors thank Assoc. Prof. Dr. Sevda TÜRKİŞ gratefully for their kind assistance and provided invaluable inputs.

Ranunculus gracilis Clarke Rizomunun In Vitro Biyolojik Aktiviteleri

Öz

Ranunculus gracilis rizomları Türkiye'nin Karabük ili Yenice Ormanlarından toplanmıştır. Ekstraksiyon çözücüsü olarak etanol seçilmiştir. Ekstraktın antimikrobiyal aktivitesinin değerlendirilmesinde filamentli ve filamentli olmayan Gram-pozitif bakteriler, Gram-negatif bakteriler ve maya suşlarını içeren disk difüzyon yöntemi kullanılmıştır. Antioksidan aktiviteyi belirlemek için DPPH serbest radikal temizleme aktivitesi ve ferrik indirgeyici antioksidan güç analizi yapılmıştır. Toplam fenolik miktarı belirlemek için Folin-Ciocalteu yöntemi ve ekstraktın toplam flavonoid içeriği için AlCl3 yöntemi seçilmiştir. Bakterilerin ortalama inhibisyon zonları (IZD) çapları 8.2 mm ile 24.45 mm arasında bulunmuştur. Bu değer mayalar için 17.82 mm ve 18.69 mm olarak ölçülmüştür. Ekstraktın toplam antioksidan aktivite değeri 7.08 mg AAE/g ekstrakt olarak hesaplanmıştır. DPPH serbest radikal süpürme aktivitesi için IC50 değeri 9.097 mg/mL olarak bulunmuştur. FRAP değeri, 1 gram numunenin indirgeme gücünün 4.66 µmol Trolox'a eşdeğer olduğunu göstermiştir. R. gracilis rizomlarının etanol ekstraktının toplam fenolik içeriği 0.414 mg GAE/g, flavonoid içeriği ise 0.68 mgQE/g olarak hesaplanmıştır. Elde edilen sonuçlara göre, R. gracilis rizomlarının analiz edilen etanolik özü, biyolojik aktivite seviyesinin önemli olarak kabul edilebileceğini göstermiştir.

Anahtar Kelimeler

Antibakteriyal etki, Antifungal aktivite, Patojenik aktinomisetler, antioksidan.

Kaynakça

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