Protective Effect of Nitrilotriacetic Acid on Lead Toxicity in Muscle and Liver Tissues of Oreochromis niloticus

Yıl 2022, , 33 - 38, 31.03.2022

Tüzün Aytekin

https://doi.org/10.35229/jaes.1060168

Öz

In this study, some enzyme activities and oxidative stress levels in the muscle and liver tissues of freshwater fish O. niloticus, which were exposed to the sublethal concentrations of Lead (Pb) and a mixture of Pb + NTA (Nitrilotriacetic acid) for 7 and 21 days were investigated. Parameters were measured with autoanalyzer devices. As a result of the data obtained; increase in total oxidant (TOS), oxidative index (OSI) levels and alanine transaminase (ALT), γ-glutamyl transferase (GGT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) enzyme activities in liver tissue, total antioxidant (TAS) level decrease was observed. In muscle tissue, an increase was observed in TOS, OSI levels and ALT enzyme activity, while TAS level and GGT, ALP enzyme activities were found to be at kontrol levels. In the presence of NTA, it was observed that the decreases or increases in some parameters were milder. As a result, we can say that Pb causes changes in the physiology of fish, and NTA partially reduces these changes.

Anahtar Kelimeler

Biochemical parameters, Lead, Nitrilotriacetic acid, Oreochromis niloticus, Total antioxidant, Total oxidan

Oreochromis niloticus’un Kas ve Karaciğer Dokularında Kurşun Toksisitesi Üzerine Nitrilotriasetik Asitin Etkisi

Öz

Bu çalışmada Kurşun (Pb)’un sublethal derişimlerinin ve Pb + NTA (Nitrilotriasetik asit) karışımının, 7 ve 21 gün süreyle, etkisine bırakılan tatlısu balığı O. niloticus’un kas ve karaciğer dokularındaki bazı enzim aktiviteleri ve oksidatif stres düzeyi incelenmiştir. Parametreler, otoanalizatör cihazlarla ölçülmüştür. Elde edilen veriler neticesinde; karaciğer dokusunda total oksidan (TOS), oksidatif indeksi (OSİ) düzeyleri ile alanin transaminaz (ALT), γ-glutamyl transferase (GGT), alkalin fosfataz (ALP) ve laktat dehidrojenaz (LDH) enzim aktivitelerinde artış, total antioksidan (TAS) düzeyinde azalış gözlenmiştir. Kas dokusunda ise TOS, OSİ düzeyleri ile ALT enzim aktivitesinde artış gözlenirken TAS düzeyi ile GGT ve ALP enzim aktivitelerinin kontrol seviyesinde olduğu saptanmıştır. NTA’nın varlığında bazı parametreler görülen azalma veya artmaların daha hafif olduğu gözlenmiştir. Sonuç olarak Pb’nin balığın fizyolojisinde değişikliklere neden olduğu, NTA’nın bu değişiklikleri kısmen de olsa azalttığı söylenebilir.

Anahtar Kelimeler

: Biyokimyasal parametreler, Lead, Nitrilotriasetik asit, Oreochromis niloticus, Total antioksidan, Total oksidan.

Kaynakça

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