Determination of Some Biological Activities of Yeast Derived Synbiotic (JD2+EPSJD2) and Postbiotic+Prebiotic (CFSJD2+EPSJD2) Applications

Year 2023, , 51 - 61, 31.03.2023

Jaafar Nozad Aakef Aakef , Zehranur Yuksekdag

https://doi.org/10.35229/jaes.1211758

Abstract

The biological potential of exopolysaccharide (EPS) depends on its chemical structure, which is affected by the fermentation conditions used in the cultivation of probiotic microorganisms. EPS produced by probiotic yeast has gained great importance in therapeutic applications such as antimicrobial, immunomodulatory, anti-inflammatory, antioxidant, anti-tumor, anti-viral, anti-diabetic, anti-ulcer, and cholesterol-lowering activities. In this study, it was aimed to conduct some biological activity (antioxidant and antibiofilm) studies of synbiotic (JD2+EPSJD2), postbiotic+prebiotic (CFSJD2+EPSJD2) applications obtained by using Pichia kudriavzevii yeast. To interpret the results obtained as effective/ineffective, commercial prebiotic inulin was used under the same conditions and the analysis results were compared. In biological activity studies determined by testing different concentrations, the highest antioxidant and antibiofilm capacity was identified in postbiotic+prebiotic (CFSJD2+EPSJD2) (86.6% and 84%, respectively) application, respectively, at 10 mg/L concentration. In addition, it was observed that the antioxidant activity (71.4%) and the capacity to prevent biofilm formation (68%)of inulin, which is used as a commercial prebiotic, were lower than the applications we used in our research.

Keywords

Pichia kudriavzevii, synbiotic, postbiotic, prebiotic, antioxidant, antibiofilm

Maya Kaynaklı Sinbiyotik (JD2+EPSJD2) ve Postbiyotik+Prebiyotik (CFSJD2+EPSJD2) Uygulamalarının Bazı Biyolojik Aktivitelerinin Belirlenmesi

Abstract

Ekzopolisakkaritin (EPS) biyolojik potansiyeli, probiyotik mikroorganizmaların yetiştirilmesinde kullanılan fermantasyon koşullarından etkilenen kimyasal yapısına bağlıdır. Probiyotik maya tarafından üretilen EPS, antimikrobiyal, immünomodülatör, anti-inflamatuar, antioksidan, anti-tümör, anti-viral, anti-diyabetik, anti-ülser ve kolesterol düşürücü aktiviteler gibi terapötik uygulamalarda çok önem kazanmıştır. Bu çalışmada, Pichia kudriavzevii mayası kullanılarak elde edilen sinbiyotik (JD2+EPSJD2), postbiyotik+prebiyotik (CFSJD2+EPSJD2) uygulamalarının bazı biyolojik aktivite (antioksidan ve antibiyofilm) çalışmalarının yapılması amaçlanmıştır. Elde edilen sonuçların etkili/etkisiz olduğunu yorumlayabilmek için aynı koşullarda ticari prebiyotik olarak satılan inülin kullanılmış ve analiz sonuçları karşılaştırılmıştır. Farklı konsantrasyonlar denenerek belirlenen biyolojik aktivite çalışmalarında 10 mg/L derişimde sırasıyla en yüksek antioksidan ve antibiyofilm kapasite postbiyotik+prebiyotik (CFSJD2+EPSJD2) (%86,6 ve %84, sırasıyla) uygulamasında tespit edilmiştir. Ayrıca, ticari prebiyotik olarak kullanılan inülinin antioksidan aktivitesinin (%71,4) ve biyofilm oluşumunu engelleme (%68) kapasitesinin araştırmamızda kullandığımız uygulamalardan daha düşük değerde olduğu gözlenmiştir.

Keywords

Pichia kudriavzevii, sinbiyotik, postbiyotik, prebiyotik, antioksidan, antibiyofilm

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