Erica arborea Bitkisi Kullanılarak Sentezlenen Çinko Nanopartiküllerin Biyoaktivitesinin Değerlendirilmesi

Yıl 2023, , 361 - 366, 30.09.2023

Yılmaz Koçak , İsmet Meydan

https://doi.org/10.35229/jaes.1301513

Öz

Çevre dostu, uygun maliyetli ve basit yöntemlerle nanopartiküllerin sentezi, tıp ve endüstri alanında pek çok araştırmaya konu olmuştur. Özellikle yeni nesil nano bazlı ilaçların geliştirilmesi için umut verici bir yaklaşım sunmaktadır. Bu çalışmada, Erica arborea (E. arborea) yaprak ekstresi stabilize edici ve indirgeyici ajan olarak kullanılarak çinko oksit nanopartiküllerin (ZnO NPs) sentezi gerçekleştirildi. Hazırlanan ZnO NP’ler UV-vis, FTIR ve SEM-EDX analizleri ile karekterizasyonu yapıldı. SEM-EDX analizlerinde nanopartiküllerin küresel şekilde olduğu ve çinko metalinin güçlü sinyalleri görüldü. UV-vis analizi, nanoparçacıkların oluşumu göstereren çözeltinin renginin açık sarıya dönüştüğünü ve 350 nm’de absorbsiyon zirvesi sergilediğini doğruladı. FT-IR, nanopartiküllerin oluşumun yaprak ekstresinin metbolitlerinin eşlik ettiğini doğruladı. E. arborea tarafından sentezlenen çinko nanopartiküller farmakolojik potansiyeli antioksidan (DPPH yöntemi), antimikrobiyal (disk diffüzyon yöntemi) ve lipid peroksidasyon (TBA yöntemi) aktiviteleri ile değerlendirildi. Biyosentezlenen ZnO NP’ler, bitki ekstresine kıyasla daha iyi antioksidan aktivite sergiledi. ZnO NP’ler ve yaprak ekstresinin DPPH radikal süpürücü aktivitesinin IC50 değerleri sırasıyla 18,71±0.35 ve 9.21±0,10 idi. ZnO NP’lerin lipid peroksidasyon inhibitör aktivitesi IC50 değeri 5,33±0.09 olarak bulundu. ZnO NP’ler, P. aeruginosa, B. Cereus, B. subtilis ve S. aureus patojen bakterileri ve C. albicans mantar şuşuna karşı duyarlı olduğu görüldü. Bulgular, doğal olarak sentezlenen çinko nanopartiküllerin sentezlenmesi, fiziksel ve kimyasal yöntemlere alternatif bir ajan olabileceğini göstermektedir. Ayrıca nanopartiküllerinin farmakolojik aktivitesi yeni nesil ilaç geliştirme çalışmalarına katkı sağlayacaktır.

Anahtar Kelimeler

Çinko oksit nanopartiküller, antimikrobiyal, antioksidan, lipid peroksidasyon, erica arborea

Proje Numarası

YOK

Assessment of the Bioactivity of Zinc Nanoparticles Synthesized Using Erica arborea Plant

Öz

The synthesis of nanoparticles by environmentally friendly, cost-effective and simple methods has been the subject of much research in medicine and industry. In particular, it offers a promising approach for the development of next-generation nano-based drugs. This study synthesized zinc oxide nanoparticles (ZnO NPs) using Erica arborea (E. arborea) leaf extract as a stabilizing and reducing agent. The prepared ZnO NPs were characterized by UV-vis, FTIR and SEM-EDX analysis. SEM-EDX analysis showed that the nanoparticles were spherical in shape and showed strong signals of zinc metal. UV-vis analysis confirmed that the color of the solution changed to light yellow, indicating the formation of nanoparticles, and exhibited an absorption peak at 350 nm. FT-IR confirmed that the formation of nanoparticles was accompanied by metabolites of the leaf extract. The pharmacological potential of zinc nanoparticles synthesized by E. arborea was evaluated by antioxidant (DPPH method), antimicrobial (disk diffusion method) and lipid peroxidation (TBA method) activities. The biosynthesized ZnO NPs exhibited better antioxidant activity compared to the plant extract. The IC50 values of DPPH radical scavenging activity of ZnO NPs and leaf extract were 18.71±0.35 and 9.21±0.10, respectively. The lipid peroxidation inhibitory activity IC50 value of ZnO NPs was found to be 5.33±0.09. ZnO NPs were found to be sensitive against pathogenic bacteria P. aeruginosa, B. cereus, B. subtilis and S. aureus and fungal strain C. albicans. The findings suggest that synthesizing naturally synthesized zinc nanoparticles could be an alternative agent to physical and chemical methods. In addition, the pharmacological activity of nanoparticles will contribute to next-generation drug development studies.

Anahtar Kelimeler

zinc oxide nanoparticles, antimikrobial, antioxidant, lipid peroxidation, Erica arborea

Destekleyen Kurum

YOK

Proje Numarası

YOK

Teşekkür

The authors would like to thank the Science Application and Research Center, Van Yuzuncu Yil University.

Kaynakça

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