Effect of Canary (Serinus canary) Semen Modified with Different Extenders on Fertilization in Artificial Insemination
Abstract
In this study, the effect of Dulbecco's Phosphate Buffered Saline (DPBS) and Dulbecco's Modified Eagle's Medium (DMEM) extenders on artificial insemination was determined.. For this purpose, the number of fertile eggs, the total number of eggs, the number of hatched eggs, and the number of hatchlings from fertile eggs were determined in the study. From these values, the fertility rate (%), egg hatch rate (%) and fertile egg hatch rate (%) were calculated. Twelve male and female canaries (Gloster breed; 1-2 years old) were used in each group. In the study, three groups, one control (C) and the other two treatment groups (T-1, T-2) were formed. Female canaries in group C were directly inseminated without using any extenders. Females in the T-1 group were diluted 1:1 with DPBS; females in the T-2 group were inseminated with semen diluted 1:1 with DMEM. Although there was a significant difference between the groups in terms of fertility rate (P<0.05), egg-laying rate and fertile egg-laying rate were not different in terms of parameters (P>0.05). The highest fertility rate (61.81%) was in group C; the lowest was in the T-1 (29.17%) group. (P<0.05). Using a DPBS extender, it was revealed that the fertility rate was negatively affected in insemination. Due to the limited number of studies on this subject, it is thought that it will be essential to investigate DPBS and DMEM diluents at different rates and methods in future studies
Keywords
Ethical Statement
Dear Managing Editor,
I would like to submit the manuscript entitled “In-Vivo Effects of Canary (Serinus Canary) Semen Diluted with Different Semen Extenders on Eggs in Artificial Insemination” in JAES
This manuscript neither the entire nor any part of its content has been published or being considered for publication elsewhere. All authors were fully involved in the study and preparation of the manuscript and they have read and approved this manuscript and take responsibility for its contents. We would like to thank the reviewers, especially the editor, who will make all the evaluations.
Sincerely.
Dr. Arda Onur ÖZKÖK
References
- Almubarak, A.M.,Kim, W., Abdelbagi, N.H., Balla, S.E., Yu, I.J. & Jeon, Y. (2021). Washing solution and centrifugation affect kinematics of cryopreserved boar semen. Journal of Animal Reproduction and Biotechnology, 36, 65-75. DOI: 10.12750/JARB.36.2.69
- Bakst, M.R. & Cecil, H.C. (1997). Techniques for semen evaluation, semen storage, and fertility determination. In: Poultry Science Association, 2nd edn, Murray R. Bakst & Julie A. Long, Buffalo, Minnesota, p. 11-15.
- Blanco, J.M., Wildt, D.E., Höfle, U., Voelker, W. & Donoghue, A.M. (2009). Implementing artificial insemination as an effective tool for ex situ conservation of endangered avian species. Theriogenology, 71, 200-213. DOI: 10.1016/j.theriogenology.2008.09.019
- Coutteel, P. (2003). Veterinary aspects of breeding management in captive passerines. In Seminars in Avian and Exotic Pet Medicine, 12, 3-10. DOI: 10.1053/saep.2003.127879
- Cramer, E.R., Ålund, M., McFarlane, S.E., Johnsen, A. & Qvarnström, A. (2016). Females discriminate against heterospecific sperm in a natural hybrid zone. Evolution, 70, 1844-1855. DOI: 10.1111/evo.12986
- Cramer, E.R., Rowe, M., Eroukhmanoff, F., Lifjeld, J.T., Sætre, G.P. & Johnsen, A. (2019). Measuring sperm swimming performance in birds: effects of dilution, suspension medium, mechanical agitation, and sperm number. Journal of Ornithology, 160, 1053-1063. DOI: 10.1007/s10336-019-01672-9
- Dumpala, P.R., Parker, H.M. & McDaniel, C.D. (2006). The effect of semen storage temperature and diluent type on the sperm quality index of broiler breeder semen. International Journal of Poultry Science, 5, 838-845. DOI: 10.3923/ijps.2006.838.845
- Donoghue, A.M. & Wishart, G.J. (2000). Storage of poultry semen. Animal Reproduction Science, 62, 213-232. DOI: 10.1016/S0378-4320(00)00160-3
- Faul, F., Erdfelder, E., Lang, A.G. & Buchner, A. (2007). G*Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behavior Research Methods, 39, 175-191.
- Fischer, D., Schneider, H., Meinecke-Tillmann, S., Wehrend, A. & Lierz, M. (2020). Semen analysis and successful artificial insemination in the St. Vincent amazon (Amazona guildingii). Theriogenology, 148, 132-139. DOI: 10.1016/j.theriogenology.2020.03.004
