Farklı Habitatlardaki Trakya Tosbağası (Testudo hermanni) Popülasyonlarında Kan Hücre Tipleri ve Anomalileri

Yıl 2024, , 110 - 118, 31.03.2024

Begüm Boran , Bengi Baycan , Çiğdem Gül , Murat Tosunoğlu

https://doi.org/10.35229/jaes.1398305

Öz

Testudo hermanni, doğada küresel olarak nesli tehlike altında olarak kabul edilmektedir. Bu çalışmada, Trakya Bölgesi’nde iki farklı habitat tipinde bulunan Testudo hermanni popülasyonlarının lökosit formülleri, nüklear anormallikleri ve eritrosit morfolojisi anormallikleri tespit edilerek habitatların kan hücreleri üzerinde potansiyel bir etkiye sahip olup olmadığı belirlenmiştir. Kırklareli/Karakoç’taki açık bir habitattan 18 ve Edirne/Keşan’daki izole bir habitattan 19 olmak üzere toplam 37 kaplumbağa örneği üzerinde çalışılmıştır. Genotoksik hasarı tespit eden Mikronukleus Testi’nden elde edilen sonuçlara göre iki habitat arasında istatistiksel olarak anlamlı bir fark bulunmamıştır. Ancak, çekirdeksiz eritrosit, nüklear kayma ve eliptik şekil bozukluğu gibi eritrosit morfolojisi anormalliklerinde ve monosit ile eozinofil yüzdesinde iki popülasyon arasında anlamlı farklar olduğu tespit edilmiştir. Vücut büyüklükleri ile diğer parametreler arasında korelasyonlar tespit edilmiştir. Bu çalışma, ülkemizde Testudo hermanni’nin nüklear anormalliklerinin incelendiği ilk çalışmadır.

Anahtar Kelimeler

Genotoksikoloji, Mikronükleus Testi, Testudo hermanni, Trakya Bölgesi

Etik Beyan

Yapılan çalışmalar için Çanakkale Onsekiz Mart Üniversitesi Hayvan Deneyleri Etik Kurulu'ndan (karar no: 2023/01-06) gerekli izinler alınmıştır.

Destekleyen Kurum

Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

FDK-2021-3555.

Teşekkür

Bu çalışma Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırma Koordinasyon Birimi (Proje Numarası: FDK-2021-3555) tarafından desteklenmiştir.

Blood Cell Types and Abnormalities in Free Ranging Hermann’s Tortoise (Testudo hermanni) Populations from Different Habitats

Öz

Testudo hermanni is considered near threatened globally in the wild. In this study differential leukocyte percentages, nuclear abnormalities, and erythrocyte morphology abnormalities of Testudo hermanni populations from two different habitat types in the Thrace Region were determined to whether habitats have a potential effect on blood cells. A total of 37 tortoises, 18 from an open habitat in Kırklareli/Karakoç, and 19 from an isolated habitat in Edirne/Keşan were studied. According to the results obtained from the Micronucleus Test, which detects genotoxic damage, there was no statistically significant difference between the two habitats. There were statistically significant differences in erythrocyte morphology abnormalities and differential leukocyte count between two populations, such as frequencies of anucleated erythrocyte, nuclear shift, elliptical shape distortion, monocyte and eosinophile percentages. This is the first study examined nuclear abnormalities of Testudo hermanni in Türkiye.

Anahtar Kelimeler

Genotoxicology, Micronucleus Test, Testudo hermanni, Thrace Region

Etik Beyan

The necessary permissions have been obtained from the Ethics Committee of Animal Experiments of Çanakkale Onsekiz Mart University (decision no: 2023/01-06) for the studies that carried out.

Destekleyen Kurum

Çanakkale Onsekiz Mart University, The Scientific Research Coordination Unit

Proje Numarası

FDK-2021-3555.

Teşekkür

This study was supported by Çanakkale Onsekiz Mart University, The Scientific Research Coordination Unit, Project Number: FDK-2021-3555.

Kaynakça

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