Evaluation of the Molecular Properties of Echinococcus granulosus Isolates from Various Hosts in Şanlıurfa Province
Abstract
Cystic echinococcosis (CE), which is raised by the larvae of Echinococcus granulosus
(E. granulosus), is single of the world's most significant zoonoses, affecting both humans and
animals. In this labor, it was the goal to perform genotyping of hydatid cyst stocks collected from
various regions of Şanlıurfa and diverse middle hosts by molecular methods. A total of 197
hydatid cyst samples were obtained, including 40 pre-diagnosed human samples with paraffin,
Türkiye 140 sheep, and 17 cattle samples. After microscopic examination, 33 human isolates and
80 sheep cyst are regarded fertile because they contain more than 250 protoscolex and/or scolex
hooks. PCR-RFLP technique was used to determine the genetics of fertile isolates by analyzing
the ribosomal DNA internal transcribed spacer gene 1 (rDNA-ITS1) region of the samples. As a
result of having the same band profile, it was found that all of the isolates were common sheep
strain (G1). Echinococcus granulosus G1 strain was the reference material. This study
demonstrated that the local sheep lineage of E. granulosus is the dominant genotype in Şanlıurfa
province. It is thought to lead to further work on the epidemiology and ecology of parasites in
animals in this region and neighboring countries.
Keywords
Cystic echinocystic, Echinococcus granulosus, genotype, PCR, RFLP
Supporting Institution
Harran University Scientific Research Projects Unit (HUBAP) supported this study with project number 014074.
Project Number
014074
Ethical Statement
Ethical approval for this study was obtained from the Harran University Medical School Ethical Board on 17.03.2014 with the number 74059997.050.01.04\039.
Thanks
We thank the Harran University Scientific Research Projects Unit [HUBAP] for its contributions. This article was produced from the thesis titled "E.granulasus genotypıng by PCR-RFLP in Şanlıurfa".
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