Investigation of Apoptosis-mediated Cytotoxic Effects of Royal Jelly on HL-60 Cells

Hasan Ulusal; Mehmet Akif Bozdayı; Hatice Kübra Yigit Dumrul; Melek Sena Tarakçıoğlu; Abdullah Taşkın

doi:10.35229/jaes.1550189

TR EN

Arı Sütünün HL-60 Hücreleri Üzerindeki Apoptoz Aracılı Sitotoksik Etkilerinin Araştırılması

Öz

Son yıllarda mevcut kanser ilaçlarının olumsuz yan etkilerini ve normal hücrelere olan toksisiteyi zararlarını azaltmak için kanser hücrelerinde etkili olabilecek nontoksik doğal ürünlerin yeni ajan olarak kullanılması bilim insanlarının dikkatini çekmektedir. Bazı in vitro ve in vivo çalışmalar, arı sütünün hücre proliferasyonunu inhibe ettiğini, apoptozu indüklediğini göstermiştir. Bu çalışmada, arı sütünün insan akut miyeloid lösemi hücre hattında proliferatif ve apoptotik süreçlerdeki etkilerini araştırmayı amaçladık. HL60 hücre hattı, 24, 48 ve 72 saat boyunca farklı konsantrasyonlarda arı sütü ile muamele edildi. 3-(4,5 dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür testi (MTT) kullanarak arı sütünün yarı maksimum inhibitör konsantrasyonu (IC50) belirlendi ve HL-60 hücrelerinin proliferasyon aktivitesi değerlendirildi. HL-60 hücrelerindeki apoptozu ölçmek için flow sitometresi analizi gerçekleştirildi. Yapılan hesaplamalarda arı sütü için IC50 değerleri 24, 48 ve 72 saat için sırasıyla 13,98, 6,45 ve 2,06 mg/mL bulundu. Flow sitometri sonuçlarıde arı sütünün bulunan konsantrasyonlarda apoptotik etkilere sahip olduğunu gösterdi. Sonuçlar, arı sütü tedavisinin HL-60 hücrelerinin proliferasyonunu önemli ölçüde azalttığını ve apoptozunu indüklediğini gösterdi. Bu çalışma, arı sütünün antikanser ve antiproliferatif etkilerinden dolayı HL-60 akut miyeloid lösemi hücrelerine karşı tedavide tamamlayıcı olarak kullanılabileceğini göstermektedir.

Anahtar Kelimeler

Arı sütü , HL-60 , Anti-kanser , Apoptoz , MTT

Investigation of Apoptosis-mediated Cytotoxic Effects of Royal Jelly on HL-60 Cells

Abstract

In recent years, the use of nontoxic natural products that can be effective on cancer cells as new agents has attracted the attention of scientists in order to reduce the negative side effects of existing cancer drugs and their toxicity to normal cells. Some in vivo and in vitro studies have shown that royal jelly (RJ) inhibits cell proliferation and induces apoptosis. In this research, we aimed to investigate the effects of RJ on proliferative and apoptotic processes in the human acute promyelocytic leukemia cell line (HL-60). The HL60 cell line was treated with different concentrations of RJ for 24, 48, and 72 hours. The half maximum inhibitory concentration (IC50) of RJ was determined using 3-(4.5 dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide test (MTT) and the proliferation activity of HL-60 cells was evaluated. Flow cytometry analysis was performed to measure apoptosis in HL-60 cells. IC50 values for RJ were calculated as 13.98, 6.45, and 2.06 mg/mL for 24, 48, and 72 hours, respectively. Flow cytometry results also showed that RJ had apoptotic effects at the concentrations found. The results showed that RJ treatment significantly induced apoptosis and reduced the proliferation of HL-60 cells. This study shows that RJ can be a complementary treatment against HL-60 acute myeloid leukemia cells due to its anticancer and antiproliferative effects.

Keywords

Royal Jelly , HL-60 , Anti-cancer , Apoptosis , MTT

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Details

Primary Language

English

Subjects

Animal Physiology - Cell

Journal Section

Research Article

Authors

Hasan Ulusal ^*
0000-0003-3890-2088
Türkiye

Mehmet Akif Bozdayı
0000-0003-0459-0629
Türkiye

Hatice Kübra Yigit Dumrul
0000-0002-6780-229X
Türkiye

Melek Sena Tarakçıoğlu
0000-0001-5649-3965
Türkiye

Abdullah Taşkın
0000-0001-8642-1567
Türkiye

Early Pub Date

December 17, 2024

Publication Date

December 31, 2024

Submission Date

September 16, 2024

Acceptance Date

October 21, 2024

Published in Issue

Year 2024 Volume: 9 Number: 4

DOI

https://doi.org/10.35229/jaes.1550189

IZ

https://izlik.org/JA99ZF74JW

APA

Ulusal, H., Bozdayı, M. A., Yigit Dumrul, H. K., Tarakçıoğlu, M. S., & Taşkın, A. (2024). Investigation of Apoptosis-mediated Cytotoxic Effects of Royal Jelly on HL-60 Cells. Journal of Anatolian Environmental and Animal Sciences, 9(4), 514-520. https://doi.org/10.35229/jaes.1550189

EBSCOHost Scilit CABI

JAES/AAS-Journal of Anatolian Environmental and Animal Sciences/Anatolian Academic Sciences&Anadolu Çevre ve Hayvancılık Dergisi/Anadolu Akademik Bilimler-AÇEH/AAS

Arı Sütünün HL-60 Hücreleri Üzerindeki Apoptoz Aracılı Sitotoksik Etkilerinin Araştırılması

Öz

Anahtar Kelimeler

Investigation of Apoptosis-mediated Cytotoxic Effects of Royal Jelly on HL-60 Cells

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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