Inhibition Effect of Some Plant Extracts on Calliteara pudibunda (Linneaus, 1758) Acetylcholinesterase

Demet Kızıl

doi:10.35229/jaes.1613209

EN TR

Inhibition Effect of Some Plant Extracts on Calliteara pudibunda (Linneaus, 1758) Acetylcholinesterase

Abstract

In pest control management, acetylcholinesterase inhibition is one of the important methods. This study determined the optimum conditions of acetylcholinesterase in C. pudibunda and investigated some of its kinetic properties. Acetylcholinesterase inhibition studies were carried out with known inhibitors of acetylcholinesterase such as tacrine, edrophonium chloride, cypermethrin and aqueous extracts of olive leaf, walnut leaf, walnut shell, cherry laurel leaf and alder leaves. The Vmax and Km values of acetylcholinesterase, which showed maximum activity at 40.0 oC and pH 7.0, were determined as 1.7±0.2 EU and 0.18±0.02 mM, respectively. In inhibition studies, the IC50 values of tacrine, edrophonium chloride and cypermethrin were found to be 6.5±0.2, 2.8± 0.3 and 6.0±0.8 μM, respectively. The IC50 values of aqueous extracts of olive leaf, alder leaf, cherry laurel leaf, walnut shell and walnut leaf were found to be 1.8± 0.2, 1.8±0.4, 1.9±0.4, 2.8±0.6 and 5.8±1.2 µg dry matter/mL, respectively. In addition, these plant extracts' oleuropein and phenolic substance amounts were determined and correlated with IC50 values. As a result, these plant extracts used in the study can be recommended as an alternative biopesticide source to control such pests through acetylcholinesterase inhibition.

Keywords

Acetylcholinesterase , biopesticides. Calliteara pudibunda (L.) , inhibition

Calliteara pudibunda (Linneaus, 1758) asetilkolinesterazi uzerinde bazi bitki ekstraktlarinin inhibisyon etkisi

Öz

Zararlı mücadele yönetiminde asetilkolinesteraz inhibisyonu önemli yöntemlerden biridir. Bu çalışmada, C. pudibunda'da asetilkolinesterazın optimum koşulları belirlenmiş ve bazı kinetik özellikleri incelenmiştir. Asetilkolinesteraz inhibisyon çalışmaları, asetilkolinesterazın bilinen inhibitörleri olan takrin, edrofonyum klorür, sipermetrin ve zeytin yaprağı, ceviz yaprağı, ceviz kabuğu, kiraz defnesi yaprağı ve kızılağaç yapraklarının sulu ekstraktları ile yürütülmüştür. 40.0 oC ve pH 7.0'de maksimum aktivite gösteren asetilkolinesterazın, Vmax ve Km değerleri sırasıyla 1.7±0.2 EU ve 0.18±0.02 mM olarak belirlenmiştir. İnhibisyon çalışmalarında kullanılan takrin, edrofonyum klorür ve sipermetrinin IC50 değerleri sırasıyla 6.5±0.2, 2.8±0.3 ve 6.0±0.8 μM olarak bulunmuştur. Zeytin yaprağı, kızılağaç yaprağı, kiraz defnesi yaprağı, ceviz kabuğu ve ceviz yaprağının sulu ekstraktlarının IC50 değerleri sırasıyla 1.8±0.2, 1.8±0.4, 1.9±0.4, 2.8±0.6 ve 5.8±1.2 µg kuru madde/mL olarak bulunmuştur. Ayrıca bu bitki ekstraktlarının oleuropein ve fenolik madde miktarları belirlenerek IC50 değerleri ile ilişkilendirilmiştir. Sonuç olarak çalışmada kullanılan bu bitki ekstraktlarının asetilkolinesteraz inhibisyonu yoluyla bu tür zararlıların kontrolünde alternatif biyopestisit kaynağı olarak önerilebileceği düşünülmektedir.

Anahtar Kelimeler

Anahtar kelimeler: Asetilkolinesteraz , biyopestisitler. Calliteara pudibunda (L.) , inhibisyon , haşere yönetimi

References

Abdellaoui, K., Boussadia, O., Miladi, M., Boughattas, I., Omri, G., Mhafdhi, M., Hazzoug, M., Acheuk, F. & Brahem, M. (2019). Olive leaf extracts toxicity to the migratory locust, Locusta migratoria: Histopathological effects on the alimentary canal and acetylcholinesterase and glutathione S-transferases activity. Neotropical Entomology, 48, 246-259. DOI: 10.1007/s13744- 018-0628-1
Abou-Donia, M.B. (2003). Organophosphorus ester- induced chronic neurotoxicity. Archives of Environmental Health, 58(8), 484–497. DOI: 10.3200/AEOH.58.8.484-497
Acet, T. (2019). A study on antioxidant properties and antimicrobial activity of various extracts of Carduus adpressus. J. Anatol. Env. Anim. Sci., 4(2), 409-413. DOI: 10.35229/jaes.619647
Akpınar, H. (2024). Halyomorpha halys’dan asetilkolinesterazın saflaştırılmasi ve inhibisyonu. Recep Tayyip Erdoğan Üniversitesi Lisansüstü Eğitim Enstitüsü. Rize, Türkiye, 41s
Akyol, A. & Sarikaya, O. (2017). Situation and evaluation of biological and chemical control applications for forest in Turkey. Applied Ecology and Environmental Research, 15(4), 341-353. DOI: 10.15666/aeer/1504_341353
Akyol, A. & Tolunay, A. (2014). Sürdürülebilir orman yönetimi ölçüt ve göstergelerinin Türkiye için modellenmesi. Turkish Journal of Forestry / Türkiye Ormancılık Dergisi, 15(1), 21. DOI: 10.18182/tjf.15028
Ansari, M., Kazemipour, M., & Fathi, S. (2011). Development of a simple green extraction procedure and HPLC method for determination of oleuropein in olive leaf extract applied to a multi- source comparative study. Journal of the Iranian Chemical Society, 8(1), 38-47. DOI: 10.1007/BF03246200
Aydogdu, M., Gokalp, F.D. & Guner, U. (2017). Toxic Effects of Pyrethrums LambdaCyhalothrin and Alpha-Cypermethrin on Pest Archips Rosana (Lepidoptera: Tortricidae) and Its Common Parasitoid. Fresenius Environmental Bulletin, 26(3), 2436-2445.
Aydoğdu, M. & Güner, U. (2012). Effects of 5 different insecticides on mortality of the Leafroller parazitoid Itoplectis maculator (Fabricius, 1775) (Ichneumonidae, Hymenoptera). Türk. Entomol. Bült., 2(4), 243-249, 2012.
Ben Hamouda, A., Boussadia, O., Khaoula, B., Laarif, A. & Braham, M. (2015). Studies on insecticidal and deterrent effects of olive leaf extracts on Myzus persicae and Phthorimaea operculella. Journal of Entomology and Zoology Studıes, 3(6), 294-297.

Cavdar, H., Senturk, M., Guney, M., Durdagi, S., Kayik, G., Supuran, C.T. & Ekinci, D. (2019). Inhibition of acetylcholinesterase and butyrylcholinesterase with uracil derivatives: kinetic and computational studies. Journal of Enzyme Inhibition and Medicinal Chemistry, 34(1), 429-437. DOI: 10.1080/14756366.2018.1543288
Céspedes, C.L., Torres, P., Marín, J.C., Arciniegas, A., Romo De Vivar, A., Pérez Castorena, A.L. & Aranda, E. (2004). Insect growth inhibition by tocotrienols and hydroquinones from Roldana barba-johannis. Phytochemistry, 65(13), 1963- 1975. DOI: 10.1016/j.phytochem.2004.03.037
Colovic, M.B., Krstic, D.Z., Lazarevic Pasti, T.D., Bondzic, A.M. & Vasic, V.M. (2013). Acetylcholinesterase inhibitors: Pharmacology and toxicology. Current Neuropharmacology, 11(3), 315-335. DOI: 10.2174/1570159x11311030006
Dinçer, B. & Akpınar, H. (2023). Purification of acetylcholinesterase from Halyomorpha halys (Stål) (Heteroptera: Pentatomidae) and management of control of the Pest through inhibition of the enzyme. International Journal of Tropical Insect Science, 43(5), 1421-1429. DOI: 10.1007/s42690-023-01047-x
Dinçer, B. & Kizil, D. (2022). Inhibition and purification of acetylcholinesterases from adults and nymphs of Ricania simulans (Walker, 1851) (Hemiptera: Ricaniidae). International Journal of Tropical Insect Science, 42(5), 3363-3372. DOI: 10.1007/s42690022-00835-1
Ding, J. & Eldridge, D. (2024). Intensifying aridity induces tradeoffs among biodiversity and ecosystem services supported by trees. Global Ecology and Biogeography, 1-13. DOI: 10.1111/geb.13894
Ding, Y., Wu, X. & Fang, J. (2011). Purification and characterization of acetylcholinesterase from brain tissues of Oreochromis aurea and its application in environmental pesticide monitoring. Sciences in Cold and Arid Regions. Dong Z.H. (1995). Purification of cholinesterase from Pseudosciaena crocea muscle. Chin Biocem J., 11(4), 487-489.
Duranay, S., Anil, S., Hasbal, G. & Özsoy, N. (2019). Purification of acethylcholinesterase from the mollusc Mytilus galloprovincialis Lam. and investigation of its kinetic properties. Journal of Research in Pharmacy, 23(4), 759-769. DOI: 10.12991/jrp.2019.185
Ellman, G.L., Courtney, K.D., Andres, V. & Featherstone, R.M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7(2), 2-3. DOI: 10.1016/0006- 2952(61)90145-9
Farag, M.R., Alagawany, M., Bilal, R.M., Gewida, A.G.A., Dhama, K., Abdel-Latif, H.M.R., Amer, M.S., Rivero-Perez, N., Zaragoza- Bastida, A., Binnaser, Y.S., Batiha, G.E.S. & Naiel, M.A.E. (2021). An overview on the potential hazards of pyrethroid insecticides in fish, with special emphasis on cypermethrin toxicity. Animals, 11(7). DOI: 10.3390/ani11071880
Gao, J., Rao, J.V., Wilde, G.E. & Zhu, K.Y. (1998). Purification and kinetic analysis of acetylcholinesterase from Western Corn Rootworm, Diabrotica virgifera virgifera (Coleoptera : Chrysomelidae ). Arch. Insect. Biochem. Physiol., 39(3), 118-125. DOI: 10.1002/(SICI)1520-6327(1998)39:3<118::AID- ARCH4>3.0.CO;2-6
Göktürk, T. & Aksu, Y. (2005). Artvin ili ormanlık alanlarında tespit edilen Calliteara pudibunda (Linneaus, 1758)’nın (Lepidoptera; Lymantriidae) morfolojisi, biyolojisi ve beslenme özellikleri üzerine bir araştırma. Orman Mühendisliği, 43, 34-35.
Grdiša, M. & Gršić, K. (2013). Botanical insecticides in plant protection. Agriculturae Conspectus Scientificus, 78(2), 85-93.
Gülçin, İ., Gören, A.C., Taslimi, P., Alwasel, S.H., Kılıc, O. & Bursal, E. (2020). Anticholinergic, antidiabetic and antioxidant activities of Anatolian pennyroyal (Mentha pulegium)- analysis of its polyphenol contents by LC- MS/MS. Biocatalysis and Agricultural Biotechnology, (23 November 2019). DOI: 10.1016/j.bcab.2019.101441
Hayouni, E.A., Abedrabba, M., Bouix, M. & Hamdi, M. (2007). The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chemistry, 105(3), 1126-1134. DOI: 10.1016/j.foodchem.2007.02.010
İpekdal, K. (2022). A theoretical approach to the pale tussock moth outbreak in Turkey. Turkish Journal of Forestry Türkiye Ormancılık Dergisi, 23(3), 212-217.
Ipekdal, K. & Avcı, M. (2023). Pale tussock moth, Calliteara pudibunda (Linnaeus, 1758) (Lepidoptera; Erebidae) with a focus on its situation in Türkiye. EPPO Bulletin, 53(1), 120- 131. DOI: 10.1111/epp.12904
Jemai, H., El Feki, A. & Sayadi, S. (2009). Antidiabetic and antioxidant effects of hydroxytyrosol and oleuropein from olive leaves in alloxan-diabetic rats. J Agric Food Chem, 57(19), 8798-8804.
Keane, S. & Ryan, M.F. (1999). Purification, characterisation, and inhibition by monoterpenes of acetylcholinesterase from the waxmoth, Galleria mellonella (L.). Insect Biochemistry and Molecular Biology, 29(12), 1097-1104. DOI: 10.1016/S09651748(99)00088-0
Li, F. & Han, Z. (2002). Purification and characterization of acetylcholinesterase from cotton aphid (Aphis gossypii glover). Archives of Insect Biochemistry and Physiology, 51(1), 37-45. DOI: 10.1002/arch.10048
Li, S., Zhao, J., Huang, R., Travers, J., Klumpp- Thomas, C., Yu, W., Mackerell, A.D., Sakamuru, S., Ooka, M., Xue, F., Sipes, N.S., Hsieh, J.H., Ryan, K., Simeonov, A., Santillo, M.F. & Xia, M. (2021). Profiling the tox21 chemical collection for acetylcholinesterase inhibition. Environmental Health Perspectives, 129(4). DOI: 10.1289/EHP6993
Lineweaver, H. & Burk, D. (1934). The determination of Enzyme Dissociation Constants. Journal of the American Chemical Society, 56. DOI: 10.1021/ja01318a036
Lister, E. & Wilson, P. (2001). Measurement of total phenolics and ABTS assay for antioxidant activity (personal communication). Crop Research Institute, Lincoln, New Zealand, 7, 235-239.
Mazzoglio, P.J., Paoletta, M., Patetta, A. & Currado, I. (2005). Calliteara pudibunda (Lepidoptera Lymantriidae) in Northwest Italy. Bulletin of Insectology, 58(1), 25-34.
Mdegela, R.H., Mosha, R.D., Sandvik, M. & Skaare, J.U. (2010). Assessment of acetylcholinesterase activity in Clarias gariepinus as a biomarker of organophosphate and carbamate exposure. Ecotoxicology, 19(5), 855-863.
Meng, X., Li, C., Xiu, C., Zhang, J., Li, J., Huang, L., Zhang, Y. & Liu, Z. (2016). Identification and biochemical properties of two new acetylcholinesterases in the pond Wolf spider (Pardosa pseudoannulata). PloS One, 11(6), 1- 14.
Mohamed, M.A., Abdel-Gawad, A.S., & Ghazy, A.E.M. (2007). Purification and characterization of an acetylcholinesterase from the infective juveniles of Heterorhabditis bacteriophora. Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, 146(3), 314-324. DOI: 10.1016/j.cbpc.2007.04.001
Mohamed, M.A., Shaalan, S., Ghazy, A.E.M., Ali, A.A., Abd-Elaziz, A.M., Ghanem, M.M.E. & Abd- Elghany, S.A. (2020). Purification and characterization of acetylcholinesterase in Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae). International Journal of Biological Macromolecules, 147, 1029-1040. DOI: 10.1016/j.ijbiomac.2019.10.071
Moores, G.D., Devine, G. J. & Devonshire, A.L. (1994). Insecticide resistance due to insensitive acetycholinesterase in Myzus persicae and Myzus nicotianae. Proceedings Brighton Crop Protection Conference-Pests and Diseases, 1994, 413-418.
Mutunga, J. M., Anderson, T. D., Wong, D. M., Carlier, P. R. & Bloomquist, J. R. (2009). Inhibition of Blattella germanica acetylcholinesterase by Bis(n)-tacrines: Prospects for the molecular design of a selective insecticide for a household pest. ACS Symposium Series, 1015, 41-51. DOI: 10.1021/bk-2009-1015.ch004
Öztürk, N., Yüksel, B., Baysal, İ. & Akbulut, S. (2024). The determination of harmful hymenoptera and lepidoptera species in forest nurseries in the Western Black Sea Region of Türkiye. Kastamonu Üniversitesi Orman Fakültesi Dergisi, 24(1), 92-103. DOI: 10.17475/kastorman.1460642
Patočka, J., Kuča, K. & Jun, D. (2004). Acetylcholinesterase and butyrylcholinesterase important enzymes of human body. Acta Medica, 47(4), 215-229. DOI: 10.14712/18059694.2018.104
Poirier, L., Brun, L., Jacquet, P., Lepolard, C., Armstrong, N., Torre, C., Daudé, D., Ghigo, E. & Chabrière, E. (2017). Enzymatic degradation of organophosphorus insecticides decreases toxicity in planarians and enhances survival. Scientific Reports, 7(1), 15194. DOI: 10.1038/s41598-017-15209-8
Poirier, L., Jacquet, P., Plener, L., Masson, P., Daudé, D., & Chabrière, E. (2018). Organophosphorus poisoning in animals and enzymatic antidotes. Environmental Science and Pollution Research, 1-26. DOI: 10.1007/s11356-018-2465-5
Prabhakaran, S.K. & Kamble, S.T. (1996). Biochemical characterization and purification of esterases from three strains of German cockroach, Blattella germanica (Dictyoptera: Blattellidae). Archives of Insect Biochemistry and Physiology, 31(1), 73-86. DOI: 10.1002/(SICI)1520- 6327(1996)31:1<73::AID-ARCH5>3.0.CO;2-Y
Prusty, A.K., Meena, D.K., Mohapatra, S., Panikkar, P., Das, P., Gupta, S.K. & Behera, B.K. (2015). Synthetic pyrethroids (Type II) and freshwater fish culture: Perils and mitigations. International Aquatic Research, 7(3), 163–191. DOI: 10.1007/s40071-015-0106-x
Pundir, C.S. & Chauhan, N. (2012). Acetylcholinesterase inhibition-based biosensors for pesticide determination: A review. Analytical Biochemistry, 429(1), 19-31. DOI: 10.1016/j.ab.2012.06.025
Ramesh, B., Rajesh, B., Bhargav, P.R.K. & Devi, N.V. (2018). Study of Acetyl cholinesterase (Ache) Gene Expression And its Relation with RNA Content in Brain of Five Different Vertebrate Species. J Biotechnol Biochem, 4(2), 21-40. DOI: 10.9790/264X0402012140
Rampa, A., Bisi, A., Belluti, F., Gobbi, S., Valenti, P., Andrisano, V., Cavrini, V., Cavalli, A. & Recanatini, M. (2000). Acetylcholinesterase inhibitors for potential use in Alzheimer’s disease: Molecular modeling, synthesis and kinetic evaluation of 11H-indeno-[1,2-b]quinolin-10- ylamine derivatives. Bioorganic and Medicinal Chemistry, 8(3), 497-506. DOI: 10.1016/S0968- 0896(99)00306-5
Rosell, G., Quero, C., Coll, J. & Guerrero, A. (2008). Biorational insecticides in pest management. Journal of Pesticide Science, 33(2), 103-121. DOI: 10.1584/jpestics.R08-01
Sarıkaya, O. (2019). Türkiye Kayın Ormanları İçin Yeni Bir Tehdit Calliteara pudibunda (Linneaus, 1758)’nın Günümüz ve Gelecekteki Potansiyel Yayılış Alanlarının Tahmini. European Journal of Science and Technology, 4(17), 45-57. DOI: 10.31590/ejosat.621325
Sarıkaya, O., Kadıoğulları, A.İ. & Açıcı, Ö. (2021). İnegöl (Bursa) ve Domaniç (Kütahya) yöresi doğu kayını (Fagus orientalis Lipsky) ormanları için önemli bir tehdit: Kızılkuyruklu Kayın Tırtılı - Calliteara pudibunda (Linneaus, 1758). European Journal of Science and Technology, 21, 131-135. DOI: 10.31590/ejosat.798622
Shi, S. & Zhang, J. (1981). Purification and some properties of cholinesterase from Nebia albiflora muscle. Acta Biochim Biophys Sin., 13(1), 10-11.
Singh, R., Firdous, S. & Sharma, P. (2014). Neurotoxic effect of cypermethrin and protective role of resveratrol in Wistar rats. International Journal of Nutrition, Pharmacology, Neurological Diseases, 4(2), 104. DOI: 10.4103/2231-0738.129598
Son, J.Y., Shin, S., Choi, K.H. & Park, I.K. (2002). Purification of soluble acetylcholinesterase from Japanese quail brain by affinity chromatography. International Journal of Biochemistry and Cell Biology, 34(2), 204-210. DOI: 10.1016/S13572725(01)00082-6
Soto-Mancera, F., Arellano, J.M. & Albendín, M.G. (2020). Carboxylesterase in Sparus aurata: Characterisation and sensitivity to organophosphorus pesticides and pharmaceutical products. Ecological Indicators, 109, 105603. DOI: 10.1016/j.ecolind.2019.105603
Trofimova, Т.А. (2012). A review of the species of Calliteara Butler, 1881 (Lepidopterа: Lymantriidae) in Russia with some taxonomic remarks. Eversmannia, 31(32), 49-61.
Vinotha Alex, A. & Mukherjee, A. (2021). Review of recent developments (2018-2020) on acetylcholinesterase inhibition based biosensors for organophosphorus pesticides detection. Microchemical Journal, 161, 105779. DOI: 10.1016/j.microc.2020.105779
Wang, Q., Li, J., Zhu, X., Sun, C., Teng, J., Chen, L., Shan, E. & Zhao, J. (2022). Microplastics in fish meals: An exposure route for aquaculture animals. Science of the Total Environment, 807, 151049. DOI: 10.1016/j.scitotenv.2021.151049
Zari, T.A. & Al-Attar, A. M. (2011). Therapeutic effects of olive leaves extract on rats treated with a sublethal concentration of carbendazim. European Review for Medical and Pharmacological Sciences, 15(4), 413-426.
Zhu, M., Xin, Y., Sun, M. & Fang, Y. (1993). Purification and properties of acetylcholinesterase from human brain. Science in China. Series B, Chemistry, Life Sciences & Earth Sciences, 36(10), 1207-1215.

Details

Primary Language

English

Subjects

Conservation and Biodiversity

Journal Section

Research Article

Authors

Demet Kızıl ^*
0000-0002-2346-7514
Türkiye

Early Pub Date

March 16, 2025

Publication Date

March 27, 2025

Submission Date

January 4, 2025

Acceptance Date

February 21, 2025

Published in Issue

Year 2025 Volume: 10 Number: 2

DOI

https://doi.org/10.35229/jaes.1613209

IZ

https://izlik.org/JA99UR24FK

APA

Kızıl, D. (2025). Inhibition Effect of Some Plant Extracts on Calliteara pudibunda (Linneaus, 1758) Acetylcholinesterase. Journal of Anatolian Environmental and Animal Sciences, 10(2), 151-158. https://doi.org/10.35229/jaes.1613209

Cited By

Doğu Karadeniz’in Önemli Bir Tarım Zararlısı Orosanga japonica (Melichar, 1898) (Homoptera: Ricaniidae)’ya Karşı Bitkisel Asetilkolinesteraz İnhibitörlerinin Değerlendirilmesi Değerlendirilmesi

Journal of Anatolian Environmental and Animal Sciences

https://doi.org/10.35229/jaes.1862726

EBSCOHost Scilit CABI

JAES/AAS-Journal of Anatolian Environmental and Animal Sciences/Anatolian Academic Sciences&Anadolu Çevre ve Hayvancılık Dergisi/Anadolu Akademik Bilimler-AÇEH/AAS