İzmir Körfezi (Türkiye) Akivadesleri (Ruditapes decussatus L.) Tarafından Toxoplasma gondii ve Cryptosporidium parvum Ookistlerinin Alımının Moleküler Tespiti

Year 2025, Volume: 10 Issue: 4, 338 - 344, 31.07.2025

Esat Çilli , Aynur Lök

https://doi.org/10.35229/jaes.1650934

Abstract

Araştırma, İzmir Körfezi'ndeki akivadeslerde (Ruditapes decussatus L.) Toxoplasma gondii ve Cryptosporidium parvum ookistlerinin alımına ve birikimine odaklanmıştır. T. gondii ve C. parvum ookistlerinin moleküler tespiti geleneksel PCR yöntemiyle yapıldı. İncelenen 120 havuzda C. parvum'un pozitiflik oranı %3,33, T. gondii'nin ise %4,16 olarak bulundu. İstatistiksel analiz, C.parvum'un aylık pozitiflik oranı ile akivades uzunluğu arasında hafif pozitif bir ilişki olduğunu ortaya çıkardı. Ancak akivades uzunluğu ile T. gondii'nin aylık pozitiflik oranı arasında istatistiksel olarak anlamlı bir korelasyon tespit edilmedi. Temiz deniz suyunda kabuklu deniz ürünleri yetiştiriciliği, tüketimden önce arındırma ve T. gondii ve C. parvum gibi yeni ortaya çıkan enterik protozoan parazitlere yönelik testlerin eklenmesiyle sıkı gıda güvenliği kontrolü tavsiye edilmektedir.

Keywords

akivades , Cryptosporidium parvum , gıda güvenliği , su kirliliği , Toxoplasma gondii

Project Number

TAGEM/HSGYAD/T1/23/A6/P3/6425

Molecular Detection Of Toxoplasma gondii And Cryptosporidium parvum Oocysts Uptake By Carpet Shell Clams (Ruditapes decussatus L.) From İzmir Bay (Türkiye)

Abstract

The research focused on uptake and accumulation of Toxoplasma gondii and Cryptosporidium parvum oocysts in carpet shell clams (Ruditapes decussatus L.) from İzmir Bay. Molecular identification of T. gondii and C. parvum oocysts was carried out by conventional PCR method. The rate of positiveness of C. parvum was 3.33 % and of T. gondii 4.16 % in 120 pools examined. Statistical analysis revealed slight positive correlation between the monthly rate of positiveness of C. parvum and the clam length. However no statistically significant correlation between clam length and T. gondii monthly rate of positiveness was detected. Shellfish farming in non polluted seawater, depuration before consumption and strict food safety control for emerging enteric protozoan parasites such as T. gondii and C. parvum are recommended.

Keywords

carpet shell clam , Cryptosporidium parvum , food safety , Toxoplasma gondii , water pollution

Ethical Statement

Experimental Animals Ethical Commitee of İzmir Bornova Veterinary Control Institute, upon application and evaluation of Esat Çilli’s PhD proposal “İzmir Körfezinde Akivadeslerde (Ruditapes decussatus (Linnaeus, 1758)) Parazit Faunasının Araştırılması/ Investigation of the Parasite fauna in Grooved Carpet Shell (Ruditapes decussatus (Linnaeus, 1758)) in Izmir Bay”, made the decision on 5th September 2023 that there is no need for ethical approval.

Supporting Institution

Republic of Türkiye Ministry of Agriculture and Forestry under TAGEM grant scheme for the year 2024

Project Number

TAGEM/HSGYAD/T1/23/A6/P3/6425

Thanks

ACKNOWLEDGEMENTS Authors are grateful to the laboratory support provided by İzmir Bornova Veterinary Control Institute staff and especially Dr. Çağatay Nuhay for providing technical support during the PCR experiments. CONFLICT OF INTEREST: The authors declared that there are no conflicts of interest regarding the publication of this article. Views and opinions expressed are however those of the authors only and do not necessarily reflect those of the Republic of Türkiye Ministry of Agriculture and Forestry. Neither the Republic of Türkiye Ministry of Agriculture and Forestry can be held responsible for them.

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