Isolation of Aeromonas sobria from Fish, Determination of Antibiotic Resistances and Genotyping
Abstract
This study aimed to isolate Aeromonas sobria strains from fish, determine their antibiotic resistance profiles, and assess their phylogenetic similarities. In 2023, 100 fish samples were collected from the Izmir region. Isolates were identified using the VITEK-2 automated system, while antibiotic resistance was evaluated through the Kirby-Bauer disk diffusion technique. Genotyping was performed using the RAPD-PCR method. Among the samples, 11% tested positive for A. sobria. Antibiograms revealed the highest resistance to ampicillin and amoxicillin (90.9%), while enrofloxacin demonstrated the highest sensitivity (72.7%). Genotyping identified 11 distinct genotypes, with phylogenetic similarities ranging from 17% to 92%. The findings highlight the zoonotic potential and diverse resistance patterns of A. sobria , emphasizing the importance of selecting appropriate antimicrobials for treatment.
Keywords
Aeromonas sobria , Antibiotic resistance , Fish , Genotyping
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