Differentiated Protein and Lipid Composition Profiles of Breast Cancer Cells Grown on Various ECM Proteins

Ediz Sarıışık; Fatma Küçük Baloğlu

doi:10.35229/jaes.1848936

TR EN

Çeşitli ECM Proteinleri Üzerinde Büyüyen Meme Kanseri Hücrelerinin Farklılaşan Protein ve Lipid Kompozisyon Profilleri

Abstract

Kötü huylu kanser hücrelerinin gelişimi, hücre dışı matris (ECM) proteinlerinin işlevine bağlıdır. Hücreler malign transformasyona uğradıkça, diğer doku ve organlara göç edebilmek için bazal membranlar gibi belirli bariyerleri aşmaları gerekir. Bu bariyerler öncelikle ECM proteinlerinden oluşur. Bu nedenle, kanser hücreleri ile bu ECM proteinleri arasındaki etkileşimin malignite gelişiminde kritik öneme sahip olduğu açıktır. Bu çalışmada, MCF7 meme kanseri hücreleri fibronektin, kolajen ve laminin olmak üzere üç farklı ECM proteini ile kaplanmış yüzeylerde yetiştirilmiştir. Daha sonra, hücreler hasat edilmiş ve zayıflatılmış toplam yansıma kızılötesi spektroskopisi (ATR-FTIR) kullanılarak farklı ECM proteinleriyle etkileşimin moleküler bileşimleri üzerindeki etkisini belirlemek için incelenmiştir. Çalışmanın sonuçları, hücreler kolajen ve fibronektin proteinleri üzerinde yetiştirildiğinde lipid biyosentezi sürecinde önemli bir artış ve/veya lipid yıkımında önemli bir azalma olduğunu göstermektedir. Fibronektin üzerinde yetiştirilen hücreler, laminin ve kontrol hücreleri üzerinde yetiştirilenlere kıyasla belirgin şekilde yüksek protein ve nükleik asit içeriği sergilemiştir. Bununla birlikte, lipidlerin proteinlere oranı fibronektin üzerinde büyütülen hücrelerde kontrol grubuna ve kolajen ve laminin üzerinde büyütülen hücrelere göre önemli ölçüde daha düşüktü. Temel bileşen analizi (PCA), ECM proteinleri üzerinde yetiştirilen hücreler ile ECM proteinleri üzerinde yetiştirilmeyen hücreler arasında önemli spektral farklılıklar olduğunu göstermiştir. Deneysel uygulamalar kontrolden başarılı bir şekilde ayırt edilmiştir.

Keywords

Meme kanseri, Fibronektin, Kolajen, Laminin, ATR-FTIR Spektroskopisi.

Differentiated Protein and Lipid Composition Profiles of Breast Cancer Cells Grown on Various ECM Proteins

Abstract

The development of malignant cancer cells is dependent on the function of extracellular matrix (ECM) proteins. As cells undergo malignant transformation, they must overcome specific barriers, such as basement membranes, in order to migrate to other tissues and organs. These barriers are primarily composed of ECM proteins. It is therefore evident that the interaction between cancer cells and these ECM proteins is of critical importance in the development of malignancy. In this study, MCF7 breast cancer cells were cultivated on surfaces coated with three distinct ECM proteins: fibronectin, collagen, and laminin. Subsequently, the cells were harvested and examined to ascertain the impact of interaction with distinct ECM proteins on their molecular composition, utilising attenuated total reflection infrared spectroscopy (ATR-FTIR). The results of the study demonstrate a significant enhancement in the process of lipid biosynthesis and/or a substantial reduction in lipid degradation when cells are cultivated on collagen and fibronectin proteins. The cells grown on fibronectin exhibited a markedly elevated protein and nucleic acid content in comparison to those grown on laminin and the control cells. However, the ratio of lipids to proteins was significantly lower in cells grown on fibronectin than in the control group and in cells grown on collagen and laminin. The principal component analysis (PCA) demonstrated that there were considerable spectral discrepancies between the cells grown on ECM proteins and those not grown on ECM proteins. The experimental treatments were successfully differentiated from the control.

Keywords

Breast cancer, Fibronectin, Collagen, Laminin, ATR-FTIR spectroscopy

Supporting Institution

Giresun University

Project Number

FEN-BAP-A-230218-22

Ethical Statement

The author declares that this study complies with Research and Publication Ethics.

Thanks

The present study was executed with funds provided by Giresun University-BAP (project no: FEN-BAP-A-230218-22).

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Details

Primary Language

English

Subjects

Cell and Nuclear Division

Journal Section

Research Article

Authors

Ediz Sarıışık ^*
0000-0003-3816-5970
Türkiye

Fatma Küçük Baloğlu
0000-0002-5435-5128
Türkiye

Publication Date

February 6, 2026

Submission Date

December 25, 2025

Acceptance Date

January 26, 2026

Published in Issue

Year 2026 Number: 2026

DOI

https://doi.org/10.35229/jaes.1848936

IZ

https://izlik.org/JA92FE36HH

APA

Sarıışık, E., & Küçük Baloğlu, F. (2026). Differentiated Protein and Lipid Composition Profiles of Breast Cancer Cells Grown on Various ECM Proteins. Journal of Anatolian Environmental and Animal Sciences, 2026, 1-8. https://doi.org/10.35229/jaes.1848936

AMA

1.Sarıışık E, Küçük Baloğlu F. Differentiated Protein and Lipid Composition Profiles of Breast Cancer Cells Grown on Various ECM Proteins. JAES. 2026;(2026):1-8. doi:10.35229/jaes.1848936

Chicago

Sarıışık, Ediz, and Fatma Küçük Baloğlu. 2026. “Differentiated Protein and Lipid Composition Profiles of Breast Cancer Cells Grown on Various ECM Proteins”. Journal of Anatolian Environmental and Animal Sciences, nos. 2026: 1-8. https://doi.org/10.35229/jaes.1848936.

EndNote

Sarıışık E, Küçük Baloğlu F (February 1, 2026) Differentiated Protein and Lipid Composition Profiles of Breast Cancer Cells Grown on Various ECM Proteins. Journal of Anatolian Environmental and Animal Sciences 2026 1–8.

IEEE

[1]E. Sarıışık and F. Küçük Baloğlu, “Differentiated Protein and Lipid Composition Profiles of Breast Cancer Cells Grown on Various ECM Proteins”, JAES, no. 2026, pp. 1–8, Feb. 2026, doi: 10.35229/jaes.1848936.

ISNAD

Sarıışık, Ediz - Küçük Baloğlu, Fatma. “Differentiated Protein and Lipid Composition Profiles of Breast Cancer Cells Grown on Various ECM Proteins”. Journal of Anatolian Environmental and Animal Sciences. 2026 (February 1, 2026): 1-8. https://doi.org/10.35229/jaes.1848936.

JAMA

1.Sarıışık E, Küçük Baloğlu F. Differentiated Protein and Lipid Composition Profiles of Breast Cancer Cells Grown on Various ECM Proteins. JAES. 2026;:1–8.

MLA

Sarıışık, Ediz, and Fatma Küçük Baloğlu. “Differentiated Protein and Lipid Composition Profiles of Breast Cancer Cells Grown on Various ECM Proteins”. Journal of Anatolian Environmental and Animal Sciences, no. 2026, Feb. 2026, pp. 1-8, doi:10.35229/jaes.1848936.

Vancouver

1.Ediz Sarıışık, Fatma Küçük Baloğlu. Differentiated Protein and Lipid Composition Profiles of Breast Cancer Cells Grown on Various ECM Proteins. JAES. 2026 Feb. 1;(2026):1-8. doi:10.35229/jaes.1848936

EBSCOHost Scilit CABI

JAES/AAS-Journal of Anatolian Environmental and Animal Sciences/Anatolian Academic Sciences&Anadolu Çevre ve Hayvancılık Dergisi/Anadolu Akademik Bilimler-AÇEH/AAS

Çeşitli ECM Proteinleri Üzerinde Büyüyen Meme Kanseri Hücrelerinin Farklılaşan Protein ve Lipid Kompozisyon Profilleri

Abstract

Keywords

Differentiated Protein and Lipid Composition Profiles of Breast Cancer Cells Grown on Various ECM Proteins

Abstract

Keywords

Supporting Institution

Project Number

Ethical Statement

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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