Sucul Bitki Bacopa monnieri (L.) Wettst.’nin Yaprak Eksplantlarından Çoklu Sürgün Rejenerasyonu Üzerine Zeatin Ön Uygulamasının Etkisi

Yıl 2019, Cilt: 4 Sayı: 2, 161 - 165, 31.08.2019

Muhammet Doğan

https://doi.org/10.35229/jaes.557425

Öz

Bacopa monnieri
(L.) Wettst su ortamının iyileştirilmesinde ve geleneksel tıp sisteminde önemli
bir bitkidir. Bu çalışmada, B. monnieri’nin
doku kültürü teknikleri ile üretimi hedeflenmiştir. B. monneri’nin yaprak eksplantları farklı dozlarda Zeatin
(ZEA)’li solüsyonlarda (0,5-8,0 mg/L) bir saat bekletilmiş ve ardından bitki
büyüme düzenleyici içermeyen kültür ortamına aktarılmıştır. Yaprak
eksplantlarından ilk sürgün çıkışları 16.
günde 4 mg/L ZEA ön uygulamasında gözlenmiştir. Sürgün rejenerasyon frekansı
%16,66-88,89 arasında sıralanmıştır. En yüksek sürgün rejenerasyon frekansları
4 ve 8 mg/L ZEA ön uygulamasında elde edilmiştir. Genel olarak ZEA
konsantrasyonu arttıkça sürgün sayısı ve sürgün uzunluğu da artmıştır. Eksplant
başına maksimum sürgün sayısı (10,31 sürgün/eksplant) 4 mg/L ZEA ön
uygulamasında, en uzun sürgünler ise (2,02 cm) 8 mg/L ZEA ön uygulamasında
tespit edilmiştir. Rejenere sürgünler 0,25 mg/L indol-3-butirik asit (IBA)
içeren kültür ortamında köklendirildikten sonra dış koşullara başarıyla
alıştırılmıştır. Sonuç olarak, ZEA ön uygulamasının B. monnieri’nin in vitro
üretimindeki etkinliği kaydedilmiştir.  

Anahtar Kelimeler

Sucul bitki, doku kültürü, sürgün rejenerasyonu, yaprak eksplantı

Effect of Pre-treatment of Zeatin on Multiple Shoot Regeneration from Leaf Explants of Aquatic Plant Bacopa monnieri (L.) Wettst.

Öz

Bacopa
monnieri (L.) Wettst is an important plant in the remediation
of aquatic environment and in the traditional medicine system. In this study,
the propagation of B. monnieri by
tissue culture techniques was aimed. Leaf explants of B. monneri were kept in different doses of Zeatin (ZEA) solutions
(0.5-8.0 mg/L) for one hour and then transferred to culture medium without
plant growth regulator. The first shoot exits from the leaf explants were
observed at 16 days with 4 mg/L ZEA pre-treatment. The frequency of shoot
regeneration was between 16.66-88.89%. The highest shoot regeneration
frequencies were obtained at 4 and 8 mg/L ZEA pre-treatment. In general, the
number of shoots and shoot length increased as the ZEA concentrations
increased. The maximum number of shoots per explant (10.31 shoots/explant) was
determined in the 4 mg/L ZEA pre-treatment, and the longest shoot (2.02 cm) was
detected in the 8 mg/L ZEA pre-treatment. The regenerated shoots were
successfully acclimatized to the external conditions after rooting in culture
medium containing 0.25 mg/L of indole-3-butyric acid (IBA). In conclusion, the
efficacy of the ZEA pre-treatment for in
vitro propagation of B. monnieri
was recorded.

Anahtar Kelimeler

Aquatic plant, tissue culture, shoot regeneration, leaf explant

Kaynakça

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