Effects of Heavy Metals with Different Concentrations on Some Biological Properties of Hyphantria cunea Drury (Lepidoptera: Arctiidae) Larvae

Yıl 2020, Cilt: 5 Sayı: 4, 685 - 690, 31.12.2020

Elif Topkara , Oğuzhan Yanar

https://doi.org/10.35229/jaes.810468

Öz

The aim of this study was to determine the effects of zinc (Zn), iron (Fe), nickel (Ni), copper (Cu), and cobalt (Co) in different concentrations on the total consumption amount, pupal weight, pupal protein and lipid content, the development time of the last instar Hyphantria cunea. For this purpose, ten diets were prepared using 4% and 12% concentrations of each metal. A total of 11 diets were made, including one control diet. Chloroform was used to determine the pupal lipid amounts. Protein analysis was performed by semi-micro Kjeldahl method with Kjeltec Auto 1030 analyzer (Tecator, Sweden). ANOVA-Dunnet test was used for statistical analysis. With increasing iron and copper concentrations in the diet, the consumption amounts, pupal weights, pupal protein and pupal lipid amounts of the larvae decreased, while the development time was prolonged. Consumption amounts, pupal weights, pupal protein and pupal lipid amounts of the groups containing nickel and cobalt were found to be higher than the control group. It was determined that with increasing zinc concentration, consumption amounts, pupal weights, and pupal protein amounts of the larvae increased, but pupal lipid amounts decreased. In this study, it has been shown that heavy metals affect the physiological processes of H. cunea larvae.

Anahtar Kelimeler

Development time, Hyphantria cunea, lipid, protein, pupal weight

Farklı Konsantrasyonlardaki Ağır Metallerin Hyphantria cunea Drury (Lepidoptera: Arctiidae) Larvalarının Bazı Biyolojik Özelliklerine Etkileri

Öz

Bu çalışmanın amacı, farklı konsantrasyonlarda çinko (Zn), demir (Fe), nikel (Ni), bakır (Cu) ve kobaltın (Co) son instar Hyphantria cunea'nın toplam diyet tüketim miktarı, pupa ağırlığı, pupa proteini ve lipit miktarı ve gelişme zamanı üzerindeki etkilerini araştırmaktı. Bu amaçla, her bir metalin % 4 ve % 12'lik konsantrasyonları kullanılarak 10 diyet hazırlanmıştır. 1'i kontrol diyeti olmak üzere toplam 11 diyet yapılmıştır. Pupal lipit miktarlarının belirlenmesinde kloroform kullanılmıştır. Protein analizi semi-mikro Kjeldahl metodu ile Kjeltec Auto 1030 analizörü (Tecator, Sweden) ile yapılmıştır. İstatistik analizlerde ANOVA-Dunnet testi kullanılmıştır. Diyetteki artan demir ve bakır konsantrasyonları ile larvaların tüketim miktarları, pupa ağırlıkları, pupa protein ve pupa lipit miktarları azalırken, gelişme süreleri uzamıştır. Nikel ve kobalt içeren grupların tüketim miktarlarının, pupa ağırlıklarının, pupa protein ve pupa lipit miktarlarının kontrol grubundan fazla olduğu bulunmuştur. Artan çinko konsantrasyonu ile larvaların tüketim miktarlarının, pupa ağırlıklarının, pupa protein miktarlarının arttığı fakat pupa lipit miktarlarının azaldığı belirlenmiştir. Bu çalışmada, ağır metallerin H. cunea larvalarının fizyolojik süreçlerini etkilediği gösterilmiştir.

Anahtar Kelimeler

Gelişme süresi, Hyphantria cunea, lipit, protein, pupa ağırlığı

Kaynakça

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