Genetic analysis of Aphaniidae Hoedeman, 1949 (Teleostei: Cyprinodontiformes) family in Anatolia

Yıl 2021, Cilt: 6 Sayı: 4, 627 - 634, 31.12.2021

Mehmet Kuyumcu İsmail Aksu , Yusuf Bektaş

https://doi.org/10.35229/jaes.1030422

Öz

We tested the mitochondrial DNA cytochrome b gene-based (1065 bp.) phylogeny and genetic diversity of tooth-carp fish (Aphaniidae Hoedeman, 1949 family), many of which are endemic, with a very high species diversity in Anatolia. A total of 107 individuals were studied from 29 stations belonging to 19 Aphaniid species. For the Cytb gene, 44 haplotypes were identified, all of which were species-specific.
The phylogenetic relationships generated by neighbor joining, maximum likelihood and maximum parsimony methods are fully compatible with each other. The generally well supported phylogenetic tree results and genetic distance results supported a structure divided into four lineages corresponding to four genera (Anatolichthys, Paraphanius, Aphanius, and Kosswigichthys). The genetic distance between these four lineages indicated a significant value ranging from 16.6% to 23.1%. Genetic distance results interspecies indicated high genetic distances ranging from 1.9% to 24.52% (between A. villwocki and P. mentoides), except for two species groups (A. fontinalis – A. sureyanus, 0.13% and A. maeandricus – A. irregularis, 0.57%).
Our results also agree with a previous studies of the Anatolian Aphaniidae family, which showed a diversification pattern shaped by Pliocene orogenic events. The present results indicate that mitochondrial DNA cytochrome b gene sequences are effective for Aphaniidae species identification and phylogenetic analysis.

Anahtar Kelimeler

Anatolia, Cytochrome b, Phylogeny, Tooth-carp

Teşekkür

We thank to Davut Turan (Rize), Fahrettin Küçük (Isparta), Baran Yoğurtçuoğlu (Ankara), Esra Bayçelebi (Rize), Cüneyt Kaya (Rize) and Salim Serkan Güçlü (Isparta) for their assistance in the fieldwork and species identification.

Anadolu’daki Aphaniidae Hoedeman, 1949 (Teleostei: Cyprinodontiformes) ailesinin genetik analizi

Öz

Anadolu'da çok yüksek tür çeşitliliğine sahip, çoğu endemik olan dişli sazancık balıklarının (Aphaniidae Hoedeman, 1949 familyası) mitokondriyal DNA sitokrom b genine (1065 bç.) dayalı filogenisini ve genetik çeşitliliğini test ettik. 19 Aphaniid türüne ait 29 istasyondan toplam 107 örnek incelenmiştir. Cytb geni için, tümü türe özgü olan 44 haplotip tanımlandı.
Komşu birleştirme, maksimum olabilirlik ve maksimum tutumluluk yöntemleriyle oluşturulan filogenetik ilişkiler birbiriyle tam uyumlu sonuçlar verdi. Genel olarak iyi desteklenen filogenetik ağaç sonuçları ve genetik uzaklık sonuçları, dört cinse (Anatolichthys, Paraphanius, Aphanius, and Kosswigichthys) karşılık gelen dört soydan oluşan bir yapıyı destekledi. Bu dört soy grubu arasındaki genetik mesafe %16,6 ile %23,1 arasında değişen ciddi bir değer gösterdi. Türler arası genetik mesafe sonuçları ise, iki tür grubu dışında (A. fontinalis – A. sureyanus ,%0.13 ve A. maeandricus – A. irregularis ,%0.57), %1.9 ile %24.52 arasında (A. villwocki ile P. mentoides arasında) değişen yüksek genetik uzaklık göstermiştir.
Sonuçlarımız aynı zamanda, Pliyosen orojenik olaylarıyla şekillenen bir çeşitlenme modeli gösteren Anadolu Aphaniidae familyasının önceki çalışmalarıyla da uyumludur. Mevcut sonuçlar, mitokondriyal DNA sitokrom b gen dizilerinin Aphaniidae türlerinin tanımlanması ve filogenetik analizi için etkili olduğunu göstermektedir.

Anahtar Kelimeler

Anadolu, Dişli sazancık, Filogeni, Sitokrom b

Kaynakça

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