The Determination of Antibiotic Resistance and Biofilm Properties in Pseudomonas aeruginosa Isolates from Raw Milk Samples

Year 2022, Volume: 7 Issue: 1, 21 - 26, 31.03.2022

Sadik Savaşan Merve Gizem Sezener

https://doi.org/10.35229/jaes.1019978

Abstract

Abstract: In this study, it was aimed to investigate the antibiotic resistance and biofilm properties of Pseudomonas aeruginosa isolates isolated from raw milks. A total number of 300 raw milk samples were collected from several dairy plants and vendors in the provinces of Aydın (n=100), İzmir (n=100) and Muğla (n=100) in Turkey. The conventional methods were used for the isolation of suspected Pseudomonas spp. from raw milk samples. A total of 63 suspected Pseudomonas spp. were isolated and these isolates were identified as being P.aeruginosa by PCR. According to PCR results, 24 isolates were identified as P.aeruginosa. The antibiotic resistances of P. aeruginosa against 8 antibiotics were determined by Kirby-Bauer Disc Diffusion method. The antibiotic resistance rates of the isolates among enrofloxacine, ceftriaxone, ciprofloxacin, meropenem, colistin, gentamycin, and azithromycin were found as 20.84%, 75.0%, 4.16%, 37.33%, 8.34%, 12.5 % and 91.66%, respectively. When examined according to multiple antibiotic resistances, it was determined that 11 isolates (45.83%) were resistant to more than 3 antibiotic groups and were evaluated as multi-resistant. The biofilm formations of the isolates were investigated in vitro with Congo Red Agar Method. The biofilm formation ratios were determined as 37.50% with CRA method. The biofilm formation and multiple antibiotic resistance rates were found as high in the raw milk isolates of P. aeruginosa. The widespread and advanced studies thought to be performed for the lightening of this situation and biofilm related antibiotic resistances.

Keywords

antibiotic resistance, biofilm, Pseudomonas aeruginosa, raw milk.

Çiğ Süt Örneklerinden İzole Edilen Pseudomonas aeruginosa İzolatlarında Antibiyotik Direnci ve Biyofilm Özelliklerinin Belirlenmesi

Abstract

Bu çalışmada çiğ sütlerden izole edilen P. aeruginosa izolatlarının antibiyotik dirençlilik ve biyofilm özelliklerinin araştırılması amaçlandı. Bu amaçla Aydın (n=100), İzmir (n=100) ve Muğla (n=100) illerindeki çeşitli süt işletme ve satıcılarından toplam 300 adet çiğ süt örneği toplandı. Pseudomonas spp. izolasyonu için geleneksel yöntemler kullanıldı. Çiğ süt örneklerinden toplam 63 şüpheli Pseudomonas spp. izole edildi ve bu izolatların P.aeruginosa yönünden identifikasyonu PCR ile gerçekleştirildi. PCR sonuçlarına göre 24 izolat P.aeruginosa olarak tanımlandı. P.aeruginosa izolatlarının 8 antibiyotiğe karşı antibiyotik dirençleri Kirby-Bauer Disk Difüzyon yöntemi ile belirlendi. Enrofloksasin, seftriakson, siprofloksasin, meropenem, kolistin, gentamisin ve azitromisin izolatlarının antibiyotik direnç oranları sırasıyla %20.84, %75.0, %4.16, %37.33, %8.34, %12.5 ve %91.66 olarak bulundu. Çoklu antibiyotik dirençlerine göre incelendiğinde 11 izolatın (%45.83) 3'ten fazla antibiyotik grubuna dirençli olduğu belirlendi ve çoklu dirençli olarak değerlendirildi. İzolatların biyofilm oluşumları in vitro olarak Congo Red Agar Metodu ile araştırıldı. Biyofilm oluşum oranları CRA yöntemi ile %37.50 olarak belirlendi. P.aeruginosa'nın çiğ süt izolatlarında biyofilm oluşumu ve çoklu antibiyotik direnç oranları yüksek bulunmuştur. Bu durumun ve biyofilm kaynaklı antibiyotik dirençlerinin aydınlatılması için yaygın ve ileri çalışmalar yapılması gerektiği düşünülmüştür.

Keywords

antibiyotik direnci, biyofilm, çiğ süt, Pseudomonas aeruginosa.

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