Morin (2′,3,4′,5,7-Pentahydroxyflavon) Antioxidant Effect in Streptozotocin-Induced Diabetic Rat Brain and Heart Tissues

Year 2022, Volume: 7 Issue: 3, 257 - 262, 30.09.2022

Ahmet Beyatli Emine Gülçeri Güleç Peker Nursel Gül Şule Coşkun Cevher

https://doi.org/10.35229/jaes.1103000

Abstract

Diabetes mellitus is agreed to be among the biggest public health burdens seen at the world. Recently, the using natural products (flavonoids specially) in diabetes treatment witnessed a growing interest due to insulin's and oral anti-diabetic medicines' unfavorable side effects. The present work is studies the beneficial effects of morin (2′,3,4′,5,7-pentahydroxyflavone) on antioxidant of tissues and lipid peroxidation status in diabetic and non-diabetic rats. Diabetes associated with elevation in reactive oxygen species and deficient in antioxidant activity, which is important aspects for pathogenesis of diabetes. The role of morin on the brain and heart antioxidant markers were estimated. The diabetic rats exhibited elevated levels of TBARS, NOx and GSH levels in brain and heart tissues when compared with healthy animals. The treatments using morin significantly stopped elevation in brain and heart TBARS and NOx levels. Oral administration of morin showed significant increase in GSH level in brain tissue. These results indicated that morin exerts antioxidative activity in diabetic rats.

Keywords

Morin; Diabetes; Brain; Heart; Oxidative stress, Morin, Diabetes, Brain, Heart

Morin (2′,3,4′,5,7-Pentahidroksiflavon) Streptozotosin ile İndüklenen Diyabetik Sıçan Beyin ve Kalp Dokularında Antioksidan Etkisi

Abstract

Diyabet dünyada bilinen en önemli sağlık problemlerinden birisidir. İnsülin ve oral antidiyabetik ilaçların yan etkilerinden dolayı son yıllarda alternatif tedavilerin araştırılmasında doğal ürünler (özellikle flavonoidler) önemli bir kaynak teşkil etmektedir. Bu çalışma normal ve streptozotosin ile diyabet oluşturulan sıçanlarda morin'in (2′,3,4′,5,7-pentahydroxyflavone) doku antioksidan ve lipid perokidasyon durumu üzerindeki olası olumlu etkisini incelemek için yapılmıştır. Artan reaktif oksijen türleri ve yetersiz antioksidan aktivite diyabet ile ilişkilidir, buda diyabet patogenezinde başlıca sorumludur. Beyin ve kalp antioksidan belirteçleri üzerinede morin'in rolü değerlendirildi. Diyabetik sıçanların beyin ve kalp dokularında normal sıçanlara göre daha yüksek TBARS ve NOx düzeyleri, daha düşük GSH düzeyi gözlendi. Morin muamelesi beyin ve kalp TBARS ve Nox düzeylerindeki artışı anlamlı olarak önledi. Ayrıca morin beyin GSH seviyesinde önemli artış gösterdi. Sonuçlar morin’in diyabetik sıçanlarda antioksidan aktivite gösterdiğini belirtmektedir.

Keywords

Morin, Diyabet, Beyin, Kalp, Oksidatif stres

References

• Al-Numair, K.S., Chandramohan, G. & M.A. Alsaif. (2012). Pretreatment with morin, a flavonoid, ameliorates adenosine triphosphatases and glycoproteins in ısoproterenol-induced myocardial infarction in rats. Journal of Natural Medicines, 66(1):95–101.
• American Diabetes Association. (2008). Standards of medical care in diabetes-2008 Diabetes Care, 31 (1, Suppl. 1). S12-S54. Doi: 10.2337/Dc08-S012.
• Atlan, N., Sepici Dinçel, A. & Koca, C. (2006). Diabetes mellitus ve oksidatif stres. Türk Biyokimya Dergisi, 31(2):51–56.
• Bellamkonda, R., Rasineni K., Singareddy, S.R., Kasetti, R.B., Pasurla, R., Chippada, A.R. & Desireddy, S. (2011). Antihyperglycemic and antioxidant activities of alcoholic extract of Commiphora mukul gum resin in streptozotocin ınduced diabetic rats. Pathophysiology, 18(4):255–61.
• Bonnefont-Rousselot, D. (2002). Glucose and reactive oxygen species. Current Opinion in Clinical Nutrition & Metabolic Care, 5(5):561–68.
• Buege, J.A. & Aust, S.D. (1978). [30] Microsomal lipid peroxidation. Pp. 302–10. In Methods in enzymology, Vol. 52. Elsevier.
• Burton, G.W. (1989). Antioxidant action of carotenoids. The Journal of Nutrition, 119(1):109–11.
• El Ghoul, J., Smiri, M., Ghrab, S., Boughattas, N.A. & Ben-Attia, M. (2012). Antihyperglycemic, antihyperlipidemic and antioxidant activities of traditional aqueous extract of Zygophyllum album in streptozotocin diabetic mice. Pathophysiology, 19(1):35–42.
• Ellman, G.L. (1959). Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics, 82(1):70–77.
• Fidan, A.F., Kucukkurt, I., Yuksel, H., Ozdemir, A., Ince, S. & Dundar, Y. (2009). The effects of structurally different saponin containing plants on tissue antioxidant defense systems, lipid peroxidation and histopathological changes in streptozotocin-ınduced diabetic rats. Journal of Animal and Veterinary Advances, 8(5):920–27.
• Green, L.C., Wagner, D.A., Glogowski, J., Skipper, P.L., Wishnok, J.S. & Tannenbaum, S.R. (1982). Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Analytical Biochemistry, 126(1):131–38.
• Hansen, M. (1998). Instructor’s manual for hansen pathophysiology: foundations of disease and clinical ıntervention. WB Saunders Company.
• Karasu, Ç. (1999). Increased activity of h2o2 in aorta ısolated from chronically streptozotocin-diabetic rats: effects of antioxidant enzymes and enzyme ınhibitors. Free Radical Biology and Medicine, 27(1–2):16–27.
• Lee, H.S., Jung, K.H., Hong, S.W., Park, I.S., Lee, C., Han, H.K., Lee, D.H. & Hong, S.S. (2008). Morin protects acute liver damage by carbon tetrachloride (CCl 4) in rat. Archives of Pharmacal Research, 31(9):1160–65.
• Lenzen, S. (2008). The mechanisms of alloxan-and streptozotocin-ınduced diabetes. Diabetologia, 51(2):216–26.
• Lorenzi, M. (2007). The polyol pathway as a mechanism for diabetic retinopathy: attractive, elusive, and resilient. Journal of Diabetes Research, 2007.
• Lubin, B.H., Shohet, S.B. & Nathan, D.G. (1972). Changes in fatty acid metabolism after erythrocyte peroxidation: stimulation of a membrane repair process. The Journal of Clinical Investigation, 51(2):338–44.
• Maritim, A.C., Moore, B.H., Sanders, R.A. & Watkins III, J.B. (1999). Effects of melatonin on oxidative stress in streptozotocin-ınduced diabetic rats. International Journal of Toxicology, 18(3):161–66.
• Matkovics, B., Varga, S.I., Szabo, L. & Witas, H. (1982). The effect of diabetes on the activities of the peroxide metabolism enzymes. Hormone and Metabolic Research, 14(02):77–79.
• Miranda, K.M., Espey, M.G. & Wink, D.A. (2001). A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide, 5(1):62–71.
• Murata, M., Takahashi, A., Saito, I. & Kawanishi, S. (1999). Site-specific dna methylation and apoptosis: ınduction by diabetogenic streptozotocin. Biochemical Pharmacology, 57(8):881–87.
• Roghani, M. & Baluchnejadmojarad, T. (2010). Hypoglycemic and hypolipidemic effect and antioxidant activity of chronic epigallocatechin-gallate in streptozotocin-diabetic rats. Pathophysiology, 17(1):55–59.
• Sankaranarayanan, C. & Pari, L. (2011). Thymoquinone ameliorates chemical ınduced oxidative stress and β-cell damage in experimental hyperglycemic rats. Chemico-Biological Interactions, 190(2–3):148–54.
• Seven, A., Güzel, S., Seymen, O., Civelek, S., Bolayırlı, M., Uncu, M. & Burçak, G. (2004). Effects of vitamin e supplementation on oxidative stress in streptozotocin ınduced diabetic rats: ınvestigation of liver and plasma. Yonsei Medical Journal, 45(4):703–10.
• Sreedharan, V., Venkatachalam, K.K. & Namasivayam, N. (2009). Effect of morin on tissue lipid peroxidation and antioxidant status in 1, 2-dimethylhydrazine ınduced experimental colon carcinogenesis. Investigational New Drugs, 27(1):21.
• Srinivasan, S. & Pari, L. (2012). Ameliorative effect of diosmin, a citrus flavonoid against streptozotocin-nicotinamide generated oxidative stress ınduced diabetic rats. Chemico-Biological Interactions, 195(1):43–51.
• Stark, G.J. (2005). Functional consequences of oxidative membrane damage. The Journal of Membrane Biology, 205(1):1–16.
• Subash, S. & Subramanian, P. (2009). Morin a flavonoid exerts antioxidant potential in chronic hyperammonemic rats: a biochemical and histopathological study. Molecular and Cellular Biochemistry, 327(1–2):153.
• Tjälve, H., Wılander, E. & Johansson, E.B. (1976). Distribution of labelled streptozotocin in mice: uptake and retention in pancreatic islets. Journal of Endocrinology, 69(3):455-NP.
• Welsh, N., Eizirik, D.L. & Sandler, S. (1994). For debate nitric oxide and pancreatic p-cell destruction in ınsulin dependent diabetes mellitus: don’t take no for an answer. Autoimmunity, 18(4):285–90.
• Xie, M.X., Long, M., Liu, Y., Qin, C. & Wang, Y.D. (2006). Characterization of the ınteraction between human serum albumin and morin. Biochimica et Biophysica Acta (BBA)-General Subjects, 1760(8):1184–91.
• Yamamoto, H., Uchigata, Y. & Okamoto, H. (1981). Streptozotocin and alloxan ınduce dna strand breaks and poly (adp–ribose) synthetase in pancreatic islets. Nature, 294(5838):284–86.
• Yugarani, T., Tan, B.K.H., Teh, M. & Das, N.P. (1992). Effects of polyphenolic natural products on the lipid profiles of rats fed high fat diets. Lipids, 27(3):181–86.
• Zhang, W., Wang, Y., Yang, Z., Qiu, J., Ma, J., Zhao, Z. & Bao, T. (2011). Antioxidant treatment with quercetin ameliorates erectile dysfunction in streptozotocin-ınduced diabetic rats. Journal of Bioscience and Bioengineering, 112(3):215–18.