Kuşburnu (Rosa Canina) Meyvesinin Antioksidan Kapasitesi ve Antimikrobiyel Potansiyelinin Araştırılması

Yıl 2023, Cilt: 8 Sayı: 1, 103 - 109, 31.03.2023

Berika Tastekin , Gülay Çiftci

https://doi.org/10.35229/jaes.1240877

Cited By: 1

Öz

Yapılan bu çalışma ile Samsun ve çevresinde yetişen kuşburnu bitkisinin antioksidan ve antibakteriyel kapasitesinin araştırılması amaçlandı. Yapılan çalışmada Samsun ve çevresinde bulunan 10 farklı bölgeden kuşburnu meyveleri toplandı. Örneklerin antioksidan kapasitesinin araştırılması üzere askorbik asit miktarı gram (g) askorbik asit, toplam tanen miktarı gram tannik asit, toplam flavonoid miktarı mg kuersetin, toplam fenolik madde miktarı gram gallik asit eşdeğeri (GAE), toplam protein miktarı g bovin serum albumin (BSA)/100 g kuşburnu cinsinden; toplam antioksidan seviyesi (TAS) mmol troloks eşdeğeri/g, toplam oksidan miktarı mmol H2O2 eşdeğeri/g cinsinden hesaplandı. Kuşburnu örneklerinin antibakteriyel kapasitesinin ölçülmesi için disk difüzyon yöntemi kullanılmış, örneklerin oluşturdukları inhibisyon çapları milimetre (mm) cinsinden tespit edildi. Çalışmamızda elde edilen bulgular doğrultusunda kuşburnu örneklerinin askorbik asit miktarı 0.781-1.120 g askorbik asit/100 g kuşburnu, tanen miktarı 1.42-4.65 g tannik asit/100 g kuşburnu, flavonoid miktarı 29.5-36.3 mg kuersetin/100 g kuşburnu, fenolik madde miktarı 4.700-8.347 g GAE/100 g kuşburnu, toplam protein miktarı 0.54-0.89 g BSA/100 g kuşburnu, toplam antioksidan seviyesi 2.59-2.62 mmol troloks eşdeğer/g, toplam oksidan seviyesi 6.13-7.41 mmol/ H2O2 eşdeğeri/g aralığında bulundu, test edilen bakterilere karşı antimikrobiyal aktivite belirlenmedi. Bu araştırma Samsun ve çevresinde yetişen kuşburnu meyvesinin içerdiği askorbik asit miktarı sebebiyle antioksidan kapasitesinin yüksek olduğunu, ayrıca antibakteriyel aktivite gösterdiği belirlendi. Çalışma sonucunda, kuşburnu meyvesinin bileşimi ile sığır, kümes hayvanları ve domuz gibi çiftlik hayvanlarının yemlerine eklenmesinin olumlu etkilerinin olacağı kanaatine varıldı.

Anahtar Kelimeler

antibakteriyel aktivite, antioksidan, Kuşburnu

Destekleyen Kurum

Bu çalışma, Ondokuz Mayıs Üniversitesi tarafından Yüksek Lisans Tez Projesi olarak desteklenmiştir.

Proje Numarası

PYO.VET.1904.17.001

Antioxidant Capacity and Antibacterial Potential of Rosehip (Rosa Canina) Fruits Grown

Öz

In this study, it was aimed to investigate the antioxidant and antibacterial capacity of the rosehip plant grown in Samsun and its surroundings. In the study, rosehip fruits were collected from 10 different regions in and around Samsun. In order to investigate the antioxidant capacity of the samples, ascorbic acid amount in grams (g) ascorbic acid, total tannin amount in gram tannic acid, total flavonoid amount in mg quercetin, total phenolic substance amount in gram gallic acid equivalent (GAE), total protein amount in g bovine serum albumin (BSA) /100 g of rosehips; total antioxidant level (TAS) mmol trolox equivalent/L, total oxidant amount was calculated in mmol H2O2 equivalent/L. Disk diffusion method was used to measure the antibacterial capacity of rosehip samples, and the inhibition diameters of the samples were determined in millimeters (mm).In line with the findings obtained in our study, the amount of ascorbic acid of the rosehip samples is 0.781-1.120 g ascorbic acid/100 g of rosehip, the amount of tannin is 1.42-4.65 g of tannic acid/100 g of rosehip, the amount of flavonoids is 29.5-36.3 mg of quercetin/100 g of rosehip, the amount of phenolic substance is 4.700 -8.347 g GAE/100 g rosehip, total protein amount 0.54-0.89 g BSA/100 g rosehip, total antioxidant level 2.59-2.62 mmol trolox equivalent/L, total oxidant level 6.13-7.41 mmol/ H2O2 equivalent/L, test Antimicrobial activity against the bacteria was not determined.In this research, it was determined that the rosehip fruit grown in Samsun and its surroundings has a high antioxidant capacity due to the amount of ascorbic acid it contains, and also shows antibacterial activity. As a result of the study, it was concluded that the composition of rosehip fruit and adding it to the feeds of livestock such as cattle, poultry and pigs will have positive effects.

Anahtar Kelimeler

antibacterial activity, antioxidant, Rosehip, TAS, TOS, antibakteriyel aktivite, antioksidan, Kuşburnu

Proje Numarası

PYO.VET.1904.17.001

Kaynakça

• Adamczak, A., Buchwald, W., Zieliński, J. & Mielcarek, S. (2012). Flavonoid and organic acid content in rose hips (Rosa L., sect. Caninae DC. EM. Christ.). Acta Biologica Cracoviensia s. Botanica, 54(1), 105-112.
• AOAC. (1995). Official Methods of Analysis, 16th edn. AOAC International, Washington.
• Aptin, R., Ghavamaldin, A., Ahmad, T. & Mariamalsadat, T. (2013). Evaluation of biochemical compounds Rosa cannia L. in North of Iran (Ramsar and Tonekabon Heights). Journal of Medicinal Plants Research, 7(45), 3319-3324.
• Arık, K. (2011). Kuşburnu (Rosa canina) petallerinin genel ekstraktının ve esansiyel yağlarının antimikrobiyal aktivitelerinin araştırılması. Yüksek Lisans Tezi, Gaziosmanpaşa Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı, Tokat, 5-42 s.
• Atanassova, M. & Christova-Bagdassarian, V. (2009). Determination of tannins content by titrimetric method for comparison of different plant species. Journal of the University of Chemical Technology and Metallurgy, 44(4), 413-415.
• Balta, F. & Tekin, N. (2021). Balıklardan izole edilen bazı bakteriyel patojenlere karşı tannik asitin antibakteriyal etkisinin belirlenmesi. Journal of Anatolian Environmental and Animal Sciences, 6, 532-539.
• Barros, L., Carvalho, A.M., Morais, J.S. & Ferreira, I.C.F.R. (2010). Strawberry-tree, blackthorn and rose fruits: Detailed characterisation in nutrients and phytochemicals with antioxidant properties. Food Chemistry, 120, 247-254.
• Bradford, M.M. (1976). A rapid and sensitive for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.
• Benli, M. & Yiğit, N. (2005). Ülkemizde yaygın kullanımı olan kekik (Thymus vulgaris) bitkisinin antimikrobiyal aktivitesi. Orlab On-Line Mikrobiyoloji Dergisi, 3, 1-8.
• Blumenthal, M., Hall, T., Rister, R. & Steinhoff, B. (1998). German Commission E Monographs. Austin (TX): American Botanical Council, pp. 368-369.
• Ercişli, S. (2005). Rose (Rosa L. spp.) germplasm resources of Türkiye. Genetic Resources and Crop Evolution, 52, 787-795.
• Erel, O. (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry, 37, 277-285.
• Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38, 1103-1111.
• Fan, C., Pacier, C. & Martirosyan, D.M. (2014). Rose hip (Rosa canina L): A functional food perspective. Functional Foods in Health and Disease, 4, 493-509.
• Fattahi, S., Jamei, R. & Sarghein, S.H. (2012). Antioxidant and antiradical activities of Rosa canina and Rosa pimpinellifolia fruits from West Azerbaijan, Iran. Journal of Plant Physiology, 2, 523-529.
• Gao, X., Björk, L., Trajkovski, V. & Uggla, M. (2000). Evaluation of antioxidant activities of rosehip ethanol extracts in different test systems. Journal of the Science of Food and Agriculture, 80, 2021- 2027.
• Georgieva, S., Angelov, G. & Boyadzhieva, S. (2014). Concentration of vitamin C and antioxidant activity of rosehip extracts. Journal of Chemical Technology and Metallurgy, 49, 451-454.
• Halásová, J. & Jičínská, D. (1988). Amounts of ascorbic acid in the hips of Rosa species. Folia Geobotanica, 23, 181-185.
• Ileina, V.I. & Bogdan, I. (1992). Ascorbic acid content of infusions prepared from dried plant products. Buletinul Institututui Agronomic Clij Napoca, 46, 169-175.
• Kurucu, S. & Keskinoğlu C. (1990). Rosa (Gül) Türleri Meyvalarının Bileşimi ve Biyolojik Aktivitesi. FABAD Farmakoloji Bilm Dergisi, 15, 121-131.
• Kühn, B.F. (1992). Dyrkning og Anvendelse, 1-6.
• Mármol, I., Sánchez-de-Diego, C., Jiménez-Moreno, N., Ancín-Azpilicueta, C. & Rodríguez-Yold, M.J. (2017). Therapeutic applications of rose hips from different rosa species. International Journal of Molecular Sciences, 18, 1137.
• Moerman, D.E. (2002). Native American ethnobotany. Portland, OR: Timber Press, pp. 482-486.
• Monroe, S. & Polk, R. (2000). Antimicrobial use and bacterial resistance. Current Opinion in Microbiology, 3, 496-501.
• Montazeri, N., Baher, E., Mirzajani, F., Barami, Z. & Yousefian, S. (2011). Phytochemical contents and biological activities of Rosa canina fruit from Iran. Journal of Medicinal Plants Research, 5, 4584-4589.
• Ogah, O., Watkins, C.S., Ubi, B.E. & Oraguzie, N.C. (2014). Phenolic compounds in Rosaceae fruit and nut crops. Journal of Agricultural and Food Chemistry, 62, 9369-9386.
• Orhan Demiroğlu, D. & Hartevioğlu, A. (2013). Kuşburnu Bitkisinin Kimyasal Bileşimi ve Biyolojik Aktiviteleri. Spatula DD, 3, 23-30.
• Orhan, D.D., Hartevioğlu, A., Küpeli, E. & Yesilada, E.J. (2007). In vivo anti-inflammatory and antinociceptive activity of the crude extract and fractions from Rosa canina L. fruits. Journal of Ethnopharmacology, 112, 394-400.
• Özkan, G., Sagdic, O., Baydar, N.G. & Baydar, H. (2004). Antioxidant and antibacterial activities of Rosa damascena flower extracts. Food Science and Technology International, 10, 277-281.
• Razungles, A., Oszmianski, J. & Sapis, J.C. (1989). Determination of carotenoids in fruits of Rosa sp. (Rosa canina and Rosa rugosa) and of chokeberry (Aronia melanocarpa). Journal of Food Science and Technology, 54, 774-775.
• Roman, I., Stănilă, A. & Stănilă, S. (2013). Bioactive compounds and antioxidant activity of Rosa canina L. biotypes from spontaneous flora of Transylvania. Chemistry Central Journal, 7, 73.
• Rosu, C.M., Olteanu, Z., Truta, E., Ciornea, E., Manzu, C. & Zamfirache, M.M. (2011). Nutritional value of Rosa spp. L. and Cornus mas L. fruits, as affected by storage conditions. Analele Stiintifice ale Universitatii" Al. I. Cuza" Din Iasi. (Serie Noua). Sectiunea 2. a. Genetica si Biologie Moleculara, 12, 147.
• Singleton, V.L., Orthofer, R., Lamuela-Raventos, R.M. & Lester P. (1999). Analysis of total polyphenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178.
• Soare, R., Babeanu, C., Bonea, D. & Panita, O. (2015). The content of total phenols, flavonoids and antioxidant activity in Rosehip from the spontaneous flora from south Romania. Scientific Papers Series A Agronomy, 58, 307-314.
• Taneva, I., Petkova, N., Dimov, I., Ivanov, I. & Denev, P. (2016). Characterization of Rose Hip (Rosa canina L.) fruits extracts and evaluation of their in vitro antioxidant activity. Journal of Pharmacognostic Phytochemistry, 5, 35.
• Tumbas, V.T., Čanadanović-Brunet, J.M., Gille, L., Đilas, S.M. & Ćetković, G.S. (2012). Characterization of the free radical scavenging activity of rose hip (Rosa canina L.) extract. International Journal of Food Properties, 15, 188-201.
• Wayne, P.A. (2020). Performance standards for Antimicrobial Susceptibility Testing, 30th ed. Approved Standard-MO2-MO7-M11. The Clinical and Laboratory Standards Institute, CLSI.
• Willich, S.N., Rossnagel, K., Roll, S., Wagner, A., Mune, O., Erlendson, J., Kharazmi, A., Sörensen, H. & Winther, K. (2010). Rose hip herbal remedy in patients with rheumatoid arthritis-A randomized controlled trial. Phytomedicine, 17, 87-93.
• Yildiz, O. & Alpaslan, M. (2012). Properties of rose hip marmalades. Food Technology and Biotechnology, 50, 98.
• Yilmaz, S.O. & Ercisli, S. (2011). Antibacterial and antioxidant activity of fruits of some rose species from Turkey. Romanian Biotechnological Letters, 16, 6407-6411.
• Zhishen, J., Mengcheng, T. & Jianming, W. (1999). The determination of flavonoids contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64, 555-559.