Calliteara pudibunda (Linneaus, 1758) asetilkolinesterazi uzerinde bazi bitki ekstraktlarinin inhibisyon etkisi

Year 2025, Volume: 10 Issue: 2, 151 - 158

Demet Kızıl

https://doi.org/10.35229/jaes.1613209

Abstract

Zararlı mücadele yönetiminde asetilkolinesteraz inhibisyonu önemli yöntemlerden biridir. Bu çalışmada, C. pudibunda'da asetilkolinesterazın optimum koşulları belirlenmiş ve bazı kinetik özellikleri incelenmiştir. Asetilkolinesteraz inhibisyon çalışmaları, asetilkolinesterazın bilinen inhibitörleri olan takrin, edrofonyum klorür, sipermetrin ve zeytin yaprağı, ceviz yaprağı, ceviz kabuğu, kiraz defnesi yaprağı ve kızılağaç yapraklarının sulu ekstraktları ile yürütülmüştür. 40.0 oC ve pH 7.0'de maksimum aktivite gösteren asetilkolinesterazın, Vmax ve Km değerleri sırasıyla 1.7±0.2 EU ve 0.18±0.02 mM olarak belirlenmiştir. İnhibisyon çalışmalarında kullanılan takrin, edrofonyum klorür ve sipermetrinin IC50 değerleri sırasıyla 6.5±0.2, 2.8±0.3 ve 6.0±0.8 μM olarak bulunmuştur. Zeytin yaprağı, kızılağaç yaprağı, kiraz defnesi yaprağı, ceviz kabuğu ve ceviz yaprağının sulu ekstraktlarının IC50 değerleri sırasıyla 1.8±0.2, 1.8±0.4, 1.9±0.4, 2.8±0.6 ve 5.8±1.2 µg kuru madde/mL olarak bulunmuştur. Ayrıca bu bitki ekstraktlarının oleuropein ve fenolik madde miktarları belirlenerek IC50 değerleri ile ilişkilendirilmiştir. Sonuç olarak çalışmada kullanılan bu bitki ekstraktlarının asetilkolinesteraz inhibisyonu yoluyla bu tür zararlıların kontrolünde alternatif biyopestisit kaynağı olarak önerilebileceği düşünülmektedir.

Keywords

Anahtar kelimeler: Asetilkolinesteraz, biyopestisitler. Calliteara pudibunda (L.), inhibisyon, haşere yönetimi

Supporting Institution

Bursa Teknik Üniversitesi Merkezi Araştırma Laboratuvarı

Thanks

Calliteara pudibunda (Linneaus, 1758) türünün tanımlanmasındaki katkılarından dolayı araştırma görevlisi Tutku Gencal ve Prof. Dr. Oğuzhan Sarıkaya'ya, analizlere olanak saglayan Bursa Teknik Üniversitesi Merkezi Araştırma Laboratuvarı yöneticilerine ve beni her zaman destekleyen sevgili aileme teşekkür ederim.

Inhibition Effect of Some Plant Extracts on Calliteara pudibunda (Linneaus, 1758) Acetylcholinesterase

Abstract

In pest control management, acetylcholinesterase inhibition is one of the important methods. This study determined the optimum conditions of acetylcholinesterase in C. pudibunda and investigated some of its kinetic properties. Acetylcholinesterase inhibition studies were carried out with known inhibitors of acetylcholinesterase such as tacrine, edrophonium chloride, cypermethrin and aqueous extracts of olive leaf, walnut leaf, walnut shell, cherry laurel leaf and alder leaves. The Vmax and Km values of acetylcholinesterase, which showed maximum activity at 40.0 oC and pH 7.0, were determined as 1.7±0.2 EU and 0.18±0.02 mM, respectively. In inhibition studies, the IC50 values of tacrine, edrophonium chloride and cypermethrin were found to be 6.5±0.2, 2.8± 0.3 and 6.0±0.8 μM, respectively. The IC50 values of aqueous extracts of olive leaf, alder leaf, cherry laurel leaf, walnut shell and walnut leaf were found to be 1.8± 0.2, 1.8±0.4, 1.9±0.4, 2.8±0.6 and 5.8±1.2 µg dry matter/mL, respectively. In addition, these plant extracts' oleuropein and phenolic substance amounts were determined and correlated with IC50 values. As a result, these plant extracts used in the study can be recommended as an alternative biopesticide source to control such pests through acetylcholinesterase inhibition.

Keywords

Acetylcholinesterase, biopesticides. Calliteara pudibunda (L.), inhibition

Supporting Institution

Bursa Technical University Central Research Laboratory

Thanks

I would like to thank the research assistant Tutku Gencal and Prof. Dr. Oguzhan Sarıkaya for their contributions to the identification of Calliteara pudibunda (Linneaus, 1758), the managers of the Central Research Laboratory of Bursa Technical University for making the analyses possible, and my beloved family for always supporting me.

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