Ege Bölgesinde Yetiştirilen Subklinik Mastitisli Sığırlarda Corynebacterium pseudotuberculosis Varlığının Araştırılması ve Antimikrobiyal Duyarlılıklarıın Belirlenmesi

Year 2025, Volume: 10 Issue: 2, 202 - 207

Çağatay Nuhay

https://doi.org/10.35229/jaes.1629149

Abstract

Öz: Bu çalışmanın amacı, Ege Bölgesi’nde subklinik mastitisli sığırlarda Corynebacterium pseudotuberculosis varlığını ve bu bakterinin antimikrobiyal duyarlılığını araştırmaktır. 2024 yılında Ege Bölgesi’ndeki süt çiftliklerinden, California Mastitis Testi ile subklinik mastitis saptanan 200 süt örneği toplandı. Örnekler kültür, Gram boyama ve biyokimyasal testlerle analiz edildi. Şüpheli izolatlar, VITEK 2 sistemi kullanılarak doğrulandı ve PIP geni için PCR ile moleküler olarak tanımlandı. İzolatların antimikrobiyal duyarlılığı Kirby-Bauer Disk Difüzyon Yöntemi ile değerlendirildi. Yapılan analizlerde 7 adet Corynebacterium pseudotuberculosis izolatı tespit edildi. Antibiyogram sonuçlarına göre izolatların enrofloksasin ve gentamisine %71, amoksisilin-klavulanik aside %57, oksitetrasikline %43 duyarlı olduğu belirlendi. Sonuç olarak, Corynebacterium pseudotuberculosis düşük tespit oranlarına sahip olmasına rağmen subklinik mastitisli sığırlarda önemli bir patojen olarak değerlendirilmiştir. Antibiyotik direnci ile ilgili daha fazla çalışma yapılması, etkili tedavi stratejileri geliştirilmesi açısından büyük önem taşımaktadır.

Keywords

Antimikrobiyal direnç, Corynebacterium pseudotuberculosis, Ege Bölgesi, PCR, sığır, subklinik mastitis

Investigation of the Presence and Antimicrobial Susceptibilities of Corynebacterium pseudotuberculosis in Subclinical Mastitis in Cattle Raised in the Aegean Region

Abstract

Abstract: This study aims to investigate the presence of Corynebacterium pseudotuberculosis and its antimicrobial susceptibility in cattle with subclinical mastitis in the Aegean Region. In 2024, 200 milk samples were collected from dairy farms in the region where subclinical mastitis was identified using the California Mastitis Test. The samples were analyzed through culture, Gram staining, and biochemical testing. Suspected isolates were confirmed using the VITEK 2 system and molecularly identified by PCR targeting the PIP gene. Antimicrobial susceptibility was assessed via the Kirby-Bauer Disk Diffusion Method. Seven Corynebacterium pseudotuberculosis isolates were identified in the analysis. Antimicrobial susceptibility tests showed 71% sensitivity to enrofloxacin and gentamicin, 57% to amoxicillin-clavulanic acid, and 43% to oxytetracycline. In conclusion, although the detection rate of Corynebacterium pseudotuberculosis was low, it remains a significant pathogen in cattle with subclinical mastitis. Further studies on antibiotic resistance are essential for developing effective treatment strategies.

Keywords

Aegean Region, antimicrobial resistance, cattle, Corynebacterium pseudotuberculosis, PCR, subclinical mastitis

Ethical Statement

Ethics committee approval was obtained from Bornova Veterinary Control Institute (Date: 04.12.2024, Number: 16964053 )

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