Toxic Effect of Palladium Chloride on Blood and Spleen Tissue of Rats and Protective Role of Quercetin

Year 2025, Volume: 10 Issue: 6, 876 - 882

Çağlar Adıgüzel , Hatice Karaboduk , Fatma Gökçe Apaydın , Yusuf Kalender

https://doi.org/10.35229/jaes.1754345

Abstract

Palladium is a heavy metal whose use in the industry has increased significantly in recent years, resulting in public health problems. Quercetin is a flavanol compound with antioxidant, anti-inflammatory and anticancer properties. In this study, the protective role of quercetin (Que) against the oxidative damage caused by palladium chloride (PdCl2) in the blood and spleen tissue of rats was investigated. Four groups were formed with 6 animals in each group. Control group, Que group (30 mg/kg bw), PdCl2 group (8 mg/kg bw) and Que+PdCl2 group. For 28 days, the administration substances were given to the rats by oral gavage. At the end of the experiment, there was a significant increase in the amount of MDA in the blood and spleen tissues of rats administered PdCl2 compared to the amount of MDA in the control group (p<0.05). At the same time, a significant decrease was observed in the SOD, CAT, GPx and GST antioxidant enzyme activities of rats in the PdCl2 group compared to the antioxidant enzyme activities of rats in the control group (p<0.05). When the quercetin-treated group was compared with the PdCl2-treated group, a significant increase in SOD, CAT, GPx and GST activities was reported, while the amount of MDA in blood and spleen tissues decreased. These results indicate that quercetin is an important food supplement.

Keywords

Blood , Oxidative stress , Palladium chloride , Quercetin , Spleen

Sıçanların Kan ve Dalak Dokusu Üzerine Paladyum Klorür’ün Toksik Etkisi ve Kuersetin’in Koruyucu Rolü

Abstract

Paladyum son yıllarda endüstride kullanımı ciddi anlamda artan ve bunun sonucu olarak da halk sağlığı problemlerini meydana getiren bir ağır metaldir. Kuersetin antioksidan, anti-inflamatuar ve anti-kanser gibi özellikleri ile öne çıkan flavanol bir bileşiktir. Bu çalışmada paladyum klorürün (PdCl2) sıçanların kan ve dalak dokusunda yarattığı oksidatif hasara karşı kuersetinin (Que) korucu rolü araştırılmıştır. Her grupta 6 hayvan olacak şekilde 4 grup oluşturulmuştur. Kontrol grubu, Que grubu (30 mg/kg v.a.), PdCl2 grubu (8 mg/kg v.a.) ve Que+PdCl2 grubu. 28 gün boyunca uygulama maddeleri sıçanlara oral gavaj yoluyla verilmiştir. Deney sonunda PdCl2 uygulanan sıçanların kan ve dalak dokularındaki MDA miktarında kontrol grubundaki sıçanların MDA miktarına göre anlamlı bir artış meydana gelmiştir (p<0,05). Aynı zamanda PdCl2 grubundaki sıçanların SOD, CAT, GPx ve GST antioksidan enzim aktivitelerinde, kontrol grubundaki sıçanların antioksidan enzim aktivitelerine göre anlamlı bir azalma gözlenmiştir (p<0,05). Kuersetin uygulamalı grup ile PdCl2 uygulamalı grup karşılaştırıldığında kan ve dalak dokularındaki MDA miktarı azalırken, SOD, CAT, GPx ve GST aktivitelerinde anlamlı bir artış rapor edilmiştir. Bu sonuçlar kuersetinin önemli bir takviye gıda olduğunu göstermektedir.

Keywords

Dalak , Kan , Kuersetin , Paladyum klorür , Oksidatif stres

Ethical Statement

Tüm deney prosedürleri için Gazi Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu tarafından onay alındı (G.Ü.ET-23.064).

Thanks

Çalışmamızın deney hayvanları kısmını maddi olarak destekleyen Gazi Üniversitesi Bilimsel Araştırma Projeleri birimine teşekkür ederiz

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