Alleviation of the Germination Inhibitory Effect of Salt Stress in Pepper (Capsicum annuum L.) Seeds by Serotonin
Abstract
Salinity stress is one of the important factors affecting all growth processes, from seed germination to seedling development, plant growth, yield and quality. In this study, the effects of serotonin treatments on the germination of pepper seeds (Capsicum annum L.) under salt stress were investigated. Different doses of salt (0, 75 and 150 mM NaCl) and serotonin (S0:0 µM, S1:5 µM, S2:10 µM, S3:15 µM, S4: 20 µM) were used. The applied seeds were placed between the papers in petri dishes, watered with the prepared salt solutions and left to germinate at 25 °C. In the study, parameters related to germination percentage, germination speed, mean germination time, daily mean germination time, peak value and germination value were investigated. As a result of the research, it was determined that the germination of pepper seeds decreased in parallel with increasing salt concentrations, and this negative effect of salt stress decreased with serotonin applications. Although it changes depending on the serotonin doses, it has been observed that significant effects occur on the measured germination parameters, and the best germination was observed at S1 and S2 doses. It is thought that the application of serotonin will have positive effects on the germination of pepper seeds under salt stress, and these effects may also occur during the plant growth period.
Keywords
References
- Abbasi, B. H., Younas, M., Anjum, S., Ahmad, N., Ali, M., Fazal, H., & Hano, C. (2020). Serotonin in plant signalling and communication. In F. Baluška, S. Mukherjee & A. Ramakrishna (Eds.), Neurotransmitters in plant signaling and communication (pp. 75-92). Springer. https://doi.org/10.1007/978-3-030-54478-2_4
- Aloui, H., Souguir, M., Latique, S., & Hannachi, C. (2014). Germination and growth in control and primed seeds of pepper as affected by salt stress. Cercetari Agronomice în Moldova, 47(3), 83-95.
- Aminifard, M. H., & Bayat, H. (2020). Evaluation of seed germination and seedling growth characteristics of sweet pepper (Capsicum annuum) under salinity and drought treatment. Iranian Journal of Seed Research, 6(2), 137-149.
- Czabator, F.J. (1962). Germination value: An index combining speed and completeness of pine seed germination. Forest Science, 8, 386-395.
- Ellis, R. H., & Roberts, E.H. (1981). The quantification of ageing and survival in orthodox seeds. Seed Science and Technology, 9, 373-409.
- Erland, L. A. E., & Saxena, P. K. (2017). Beyond a neurotransmitter: The role of serotonin in plants. Neurotransmitter, 4, e1538. https://doi.org/10.14800/nt.1538
- Erland, L. A., Turi, C. E., & Saxena, P. K. (2016). Serotonin: An ancient molecule and an important regulator of plant processes. Biotechnology Advances, 34(8), 1347-1361. https://doi.org/10.1016/j.biotechadv.2016.10.002
- Gairola, K.C., Nautiyal, A.R., & Dwivedi, A.K. (2011). Effect of temperatures and germination media on seed germination of Jatropha curcas Linn. Advances in Bioresearch, 2(2), 66-71.
- He, H., Lei, Y., Yi, Z., Raza, A., Zeng, L., Yan, L., Xiaoyu, D., Yong, C., & Xiling, Z. (2021). Study on the mechanism of exogenous serotonin improving cold tolerance of rapeseed (Brassica napus L.) seedlings. Plant Growth Regulation, 94(2), 161-170. https://doi.org/10.1007/s10725-021-00700-0
- Ibrahimova, U., Kumari, P., Yadav, S., Rastogi, A., Antala, M., Suleymanova, Z., & Brestic, M. (2021). Progress in understanding salt stress response in plants using biotechnological tools. Journal of Biotechnology, 329, 180-191. https://doi.org/10.1016/j.jbiotec.2021.02.007