A Study on the Germination of Origanum acutidens L. Seeds Subjected to Pre-Treatment of Gibberellic Acid and Colchicine

Year 2023, , 152 - 158, 30.12.2023

Halit Karagöz

https://doi.org/10.56430/japro.1372119

Abstract

In Türkiye, the general name for aromatic plant species belonging to the Lamiaceae family is “thyme”. However, species containing thymol/carvacrol type essential oil are considered “thyme”. Origanum acutidens is one of the thyme species that grows endemic in the Northeastern Anatolia Region of Türkiye. The low germination rate of its seeds is one of the factors limiting the studies conducted on this plant. This study was conducted to investigate the effects of different doses of colchicine and gibberellic acid on germination in O. acutident seeds. Seeds collected from the plant’s natural habitat at the end of the flowering period were used as plant material. The experiment was planned as control (only distilled water) and treatments consisting of three different gibberellic acid (GA3) [100 ppm (GA1), 200 ppm (GA2) and 300 ppm (GA3)] and four different colchicine doses [0.01 mM (C1), 0.02 mM (C2), 0.04 mM (C3) and 0.08 mM (C4)]. The applications were kept at 25±1 ⁰C for 12 hours. After the waiting period, all seeds were filtered and placed, 50 seeds each, in 9 cm diameter petri dishes between two layers of sterile filter paper sheets. The experiment was carried out in 4 replications. Some parameters of the germination (Germination rate (GR), Germination time (GT), Average germination time (AGT)) and early seedling period (Embryonal root length (ERL), Number of embryonal roots (NER), Root fresh weight (RFW), Root dry weight (RDW), Grass sheath length (GSH)) were measured and the results were statistically evaluated. In general, the highest values obtained from all evaluated germination (92.0% GR and 1.7 day AGT) and early seedling parameters (10.4 cm ERL, 4.6 NER, 0.095 g RFW, 0.028 g RDW and 3.6 cm GSL) were found to belong to the GA3 application. The lowest values obtained from the relevant parameters were obtained with the C4 application. In our study, it was observed that gibberellic acid applications significantly increased germination in this plant and positively increased the parameters related to germination. Based on the study results, we think that colchicine stimulates germination at certain rates, but causes death by having a toxic effect in increasing doses.

Keywords

Colchicine, Endemic plant, O. acutidens, Seed germination, Thyme, Wild plant

References

• Abd El-Latif, F. M., El-Gioushy, S. F., Islam, S. E., & Zakry, T. A. (2018). Impact of papaya seed soaking in different BA, colchicine and EMS solutions on germination, growth and chromosomal behaviour. Asian Journal of Biotechnology and Genetic Engineering, 1(1), 1-17. https://doi.org/10.9734/AJBGE/2018/40538
• Aligiannis, N., Kalpoutzakis, E., Mitaku, S., & Chinou, IB. (2001). Composition and antimicrobial activity of the essential oils of two origanum species. Journal of Agricultural and Food Chemistry, 49(9), 4168-4170. https://doi.org/10.1021/jf001494m
• Arteca, R. N. (2013). Plant growth substances: Principles and applications. Springer Science & Business Media.
• Bakry, F., de la Reberdiere, N. P., Pichot, S., & Jenny, C. (2007). In liquid medium colchicine treatment induces non chimerical doubled-diploids in a wide range of mono- and interspecific diploid banana clones. Fruits, 62(1), 3–12. https://doi.org/10.1051/fruits:2006043
• Baser, K. H. C. (2002). The Turkish Origanum species. In S. Kintzios (Ed.), Oregano: The genera Origanum and Lippia (pp. 109-126). CRC Press.
• Bond, L. (1942). Colchicine stimulation of seed germination in Petunia axillaris. Journal of Heredity, 33(5), 200-201. https://doi.org/10.1093/oxfordjournals.jhered.a105167
• Cavusoğlu, K., & Kabar, K. (2007). The effects of pretreatments of some plant growth regulators on germination and seedling growth of radish seeds under saline conditions. Dumlupınar Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 14, 27–36.
• Cetin, A., Arslanbas, U., Saraymen, B., Canoz, O., Ozturk, A., & Sagdic, O. (2011). Effects of grape seed extract and Origanum onit esessential oil on cisplatin-induced hepatotoxicity in rats. International Journal of Hematology and Oncology, 28(4), 133-140. https://doi.org/10.4999/uhod.10060
• Davis, P. H. (1982). Flora of Turkey and east Aegean islands. University Press.
• Ellis, R. H., & Roberts, E. H. (1981). An investigation into the possible effects of ripeness and repeated threshing on barley seed longevity under six different storage environments. Annals of Botany, 48(1), 93-96. https://doi.org/10.1093/oxfordjournals.aob.a086103
• El-Nashar, Y. I., & Ammar, M. H. (2016). Mutagenic influences of colchicine on phenological and molecular diversity of Calendula officinalis L. Genetics and Molecular Research, 15(2), 1-15. https://doi.org/10.4238/gmr.15027745
• Gosh, P., Dash, P. K., Rituraj, S., & Mannan, M. A. (2014). Effect of salinity on germination, growth and yield of radish (Raphanus sativus L.) varieties. International Journal of Biosciences, 5(1), 37-48. https://doi.org/10.12692/ijb/5.1.37-48
• Grouh, M. S. H., Meftahizade, H., Lotfi, N., Rahimi, V., & Baniasadi, B. (2011). Doubling the chromosome number of Salvia hains using colchicine: Evaluation of morphological traits of recovered plants. Journal of Medicinal Plants Research, 5(19), 4892–4898. https://doi.org/10.5897/JMPR.9000459
• Hadlaczky, G., Bisztray, G., Praznovszky, T., & Dudits, D. (1983). Mass isolation of plant chromosomes and nuclei. Planta, 157, 278–285. https://doi.org/10.1007/BF00405195
• Ietswaart, J. H. (1980). A taxonomic revision of the genus Origanum (Labiatae). The Hague: Leiden University Press.
• İpek, A., Kaya, M. D., & Gürbüz, B., (2008). Çemen (Trigonella foenum-graecum L.) ve kimyon (Cuminum cyminum L.) tohumlarının çimlenmesi üzerine tohum yaşı ve GA3 uygulamalarının etkileri. Tarım Bilimleri Dergisi, 14(1), 57-61. (In Turkish)
• ISTA. (1996). International rules for seed testing. Seed Science and Technology.
• Jacobsen, J. V., Pearce, D. W., Poole, A. T., Pharis, R. P., & Mander, L. N. (2002). Abscisic acid, phaseic acid and gibberellin contents associated with dormancy and germination in barley. Physiologia Plantarum, 115(3), 428-441. https://doi.org/10.1034/j.1399-3054.2002.1150313.x
• Karagoz, H. (2018). Agronomic and molecular characterization of Origanum acutidens (hand.- mazz.) Ietswaart populations in northern Anatolia region (Doctoral dissertation, Atatürk University).
• Karagoz, H., & Parlakova Karagoz, F. (2019). Areas of utilization of Origanum acutidens (Hand. -Mazz) Ietswaart and Carvacrol. International Journal of Current Research and Academic Review, 7(12), 46–55. https://doi.org/10.20546/ijcrar.2019.702.006
• Karagoz, H., Hosseinpour, A., Parlakova Karagoz, F., Cakmakci, R., & Haliloglu, K. (2022). Dissection of genetic diversity and population structure in oregano (Origanum acutidens L.) genotypes based on agro-morphological properties and start codon targeted (SCoT) markers. Biologia, 77(5), 1231-1247. https://doi.org/10.1007/s11756-021-00989-2
• Karakurt, H., Aslantaş, R., & Eşitken, A. (2010). Tohum çimlenmesi ve bitki büyümesi üzerinde etkili olan çevresel faktörler ve bazı ön uygulamalar. Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 24(2), 115-128. (In Turkish)
• Khalili, S., Niazian, M., Arab, M., & Norouzi, M. (2020). In vitro chromosome doubling of African daisy, Gerbera jamesonii Bolus cv. Mini Red. Nucleus, 63(1), 59-65. https://doi.org/10.1007/s13237-019-00282-3
• Kizil, S., Ipek, A., Arslan, N., & Khawar, K. M. (2009). Some agronomical characteristics and essential oil content of oregano (Origanum onites L.) as influenced by planting densities. Journal of Essential Oil-Bearing Plants, 12(2), 172-180. https://doi.org/10.1080/0972060x.2009.10643707
• Kulkarni, G. B. (2011). Effect of mutagen on pollen fertility and other parameters in horsegram (Macrotyloma uniflorum (Lam.) Verdc). Bioscience Discovery, 2(1), 146-150.
• Kwon, S. J., Seo, D. Y., Cho, G. Y., Lee, M. S., Moon, Y. J., & Boo, H. O. (2016). Effect of colchicine on chromosome doubling in Codonopsis lanceolata. Korean Journal of Plant Resources, 29(3), 347–354. https://doi.org/10.7732/kjpr.2016.29.3.347
• Lv, Z., Zhu, F., Jin, D., Wu, Y., & Wang, S. (2021). Seed germination and seedling growth of Dendrocalumus brandisii in vitro, and the inhibitory mechanism of colchicine. Frontiers in Plant Science, 12, 784581. https://doi.org/10.3389/fpls.2021.784581
• Mangena, P. (2021). Germination, morphological and physiological evaluation of seedlings pretreated with colchicine in soybean (Glycine max L). Walailak Journal of Science and Technology (WJST), 18(18), 9489. https://doi.org/10.48048/wjst.2021.9489
• Mo, L., Chen, J., Lou, X., Xu, Q., Dong, R., Tong, Z., Huang, H., & Lin, E. (2020). Colchicine induced polyploidy in Rhododendron fortunei Lindl. Plants, 9(4), 424. https://doi.org/10.3390%2Fplants9040424
• Mufwanzala, N., & Dikinya, O. (2010). Impact of poultry manure and its associated salinity on the growth and yield of spinach (Spinacea oleracea) and carrot (Daucus carota). International Journal of Agriculture & Biology, 12, 489–494.
• Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
• Nasırcılar, A. G., Ulukapı, K., & Kurt, Z. (2020). Effects of salicylic acid on germination and vegetative growth properties of radish (Raphanus sativus L.) cultivars grown under drought stress conditions. Turkish Journal of Agriculture-Food Science and Technology, 8(11), 2293-2299. https://doi.org/10.24925/turjaf.v8i11.2293-2299.3087
• Okcu, G., Kaya, M. D., & Atak, M. (2005). Effects of salt and drought stresses on germination and seedling growth of pea (Pisum sativum L.). Turkish Journal of Agriculture and Forestry, 29(4), 237-242.
• Pande, S., & Khetmalas, M. (2012). Biological effect of sodium azide and colchicine on seed germination and callus induction in Stevia rebaudiana. Asian Journal of Experimental Biological Sciences, 3(1), 93-98.
• Sadat Noori, S. A., Norouzi, M., Karimzadeh, G., Shirkool, K., & Niazian, M. (2017). Effect of colchicine-induced polyploidy on morphological characteristics and essential oil composition of ajowan (Trachyspermum ammi L.). Plant Cell, Tissue and Organ Culture, 130, 543-551. https://doi.org/10.1007/s11240-017-1245-0
• Sari, A. O., & Altunkaya, M. (2015). Doğadan tarlaya: Kekik. TÜRKTOB Dergisi, 15, 22-27. (In Turkish)
• Sourour, A., Ameni, B., & Mejda, C. (2014). Efficient production of tetraploid barley (Hordeum vulgare L.) by colchicine treatment of diploid barley. Journal of Experimental Biology and Agricultural Sciences, 2, 113-119.
• Talei, D., & Fotokian, M. H. (2020). Improving growth indices and productivity of phytochemical compounds in lemon balm (Melissa officinalis L.) through induced polyploidy. BioTechnologia, 101(3), 215-226. https://doi.org/10.5114/bta.2020.97880
• Tepe, A., Kaya, H., Batmaz, G., Özkan, C. F., & Demirtaş, E. I. (2011). Effect of saline irrigation water applications on the yield of some pepper pure lines. Derim Journal, 28(1), 1-11.
• Tepe, B., Cakir, A., & Sihoglu Tepe, A. (2016). Medicinal uses, phytochemistry, and pharmacology of Origanum onites (L.): A review. Chemistry & Biodiversity, 13(5), 504-520. https://doi.org/10.1002/cbdv.201500069
• Trease, S. E., & Evans, D. (1983). Colchicum seed and corn - Pharmacognosy. Balliere Tindall.
• Tumen, G., Baser, K. H. C., Kirimer, N., & Ozek, T. (1995). Essential oil of Origanum saccatum P. H. Davis. Journal of Essential Oil Research, 7(2), 175-176. https://doi.org/10.1080/10412905.1995.9698493
• Yamaguchi, S., & Kamiya, Y. (2002). Gibberellins and light-stimulated seed germination. Journal of Plant Growth Regulation, 20(4), 369-376. https://doi.org/10.1007/s003440010035
• Yıldız, S., Parlakova Karagoz, F., & Dursun, A. (2017). Giberellik asit ön uygulamasına tabi tutulmuş hüsnüyusuf (Dianthus barbatus L.) tohumlarının tuz stresinde çimlenmesi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 48(1), 1-7. https://doi.org/10.17097/ataunizfd.320231 (In Turkish)