Genome-Wide Analysis and Characterization of FBA (Fructose 1,6-bisphosphate aldolase) Gene Family of Phaseolus vulgaris L

Year 2024, , 30 - 40, 31.03.2024

Sümeyra Uçar , Şeyma Alım , Ayşe Gül Kasapoğlu , Esma Yigider , Emre İlhan , Murat Turan , Aysun Polat , Neslihan Dikbaş , Murat Aydın

https://doi.org/10.56430/japro.1432135

Abstract

Fructose-1,6-biphosphate aldolase (FBA) genes have important roles in plant stress responses. At the same time, these genes positively affect growth and development in plants. FBA is involved in gluconeogenesis, glycolysis, and the Calvin-Benson cycle, and it is an enzyme that plays an important role in signal transduction of these stages. This study aims to determine and characterize the FBA gene family in the bean genome. As a result of the study, 7 Pvul-FBA genes were determined in the bean (Phaseolus vulgaris L.) genome. The highest amino acid number of Pvul-FBA proteins was determined in the Pvul-FBA-1 gene (1374), and the highest molecular weight (43.03 kDa) was determined in the Pvul-FBA-7 gene. Again, the highest isoelectric point (8.03) was determined in the Pvul-FBA-3 gene. It has been determined that the Pvul-FBA-6/Pvul-FBA-7 genes are segmental duplicated genes. The main four groups were obtained according to the phylogenetic analysis consisting of FBA proteins of three plants (P. vulgaris, Glycine max, and Arabidopsis thaliana). As a result of interproscan analysis, Motif-1, 2, 3, 4 and 5 were found to contain the fructose-bisphosphate aldolase domain. According to in silico gene expression analysis, it was determined that the expression rates of Pvul-FBA genes increased or decreased under salt and drought stress conditions. Synteny analyses of FBA genes in common bean and A. thaliana plants showed that these three plants have a relationship in terms of FBA genes. The results of this research will allow a better designation of the molecular structure of the FBA gene family in common bean.

Keywords

Fructose-bisphosphate aldolase, Gene duplication, Gene expression, In silico analysis, Phaseolus vulgaris L

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