Determination and Pathogenicity of Verticillium dahliae Isolates Obtained from Tomato Plants (Solanum lycopersicum L.) in the Iğdır Province

Tuba Genç Kesimci

doi:10.56430/japro.1433931

EN

Determination and Pathogenicity of Verticillium dahliae Isolates Obtained from Tomato Plants (Solanum lycopersicum L.) in the Iğdır Province

Abstract

This study was carried out to identification of Vegetative Compatibility Groups (VCGs) and pathogenicity of Verticillium dahliae isolates obtained from tomato plants in Iğdır province. As a result of survey studies conducted in 18 different regions, 14 isolates were obtained from 629 diseased tomato plants. In the complementation test, the seven isolates were found as VCG2A and VCG2B by using international reference isolates. VCGs of other isolates were not identified. Assessment of the aggressiveness of the KRS-2, YC-13, YY-14, and MLK3-4 isolates was evaluated on tomato (cv. Super). The disease severity was between 15-45% and MLK3-4 isolate had the highest disease severity (45%). Additionally, the effect of temperature on the growth of V. dahliae isolates (TSD-1, MLK3-4 and YY-14) was determined. The isolates showed optimal growth temperatures ranging from 20 to 25 °C (except for TSD-1). In control of fungal diseases, it is very important to describe the disease and pathogen. Therefore, the results of the study are important for tomato growers and researchers.

Keywords

Pathogenicity, Solanum lycopersicum L., VCGs, Verticillium wilt

Supporting Institution

Scientific Research Projects Coordination Unit of Iğdır University

Project Number

2018-FBE-A08

Ethical Statement

This article does not contain any studies with human or animal subjects.

Thanks

This article was supported by Scientific Research Projects Coordination Unit of Iğdır University (Project number 2018-FBE-A08).

References

Akar, H., Cat, A., & Catal, M. (2024). Molecular characterizations of Verticillium dahliae isolates from grafted tomatoes grown in Antalya province of Türkiye. Journal of Phytopathology, 172(1), e13262. https://doi.org/10.1111/jph.13262
Alsohaili, S. A., & Bani-Hasan, B. M. (2018). Morphological and molecular identification of fungi isolated from different environmental sources in the Northern Eastern desert of Jordan. Jordan Journal of Biological Sciences, 11(3), 329-337.
Bao, J. R., Katan, J., Shabi, E., & Katan, T. (1998). Vegetative-compatibility groups in Verticillium dahliae from Israel. European Journal of Plant Pathology, 104, 263-269. https://doi.org/10.1023/A:1008696312859
Bhat, R. G., & Subbarao, K. V. (1999). Host range specificity in Verticillium dahliae. Phytopathology, 89(12), 1218-1225. https://doi.org/10.1094/phyto.1999.89.12.1218
Bhat, R. G., Smith, R. F., Koike, S. T., Wu, B. M., & Subbarao, K. V. (2003). Characterization of Verticillium dahliae isolates and wilt epidemics of pepper. Plant Disease, 87(7), 789-797. https://doi.org/10.1094/pdis.2003.87.7.789
Burgess, T., Bihon, W., Wingfield, M. J., & Wingfield, B. D. (2009). A simple and rapid method to determine vegetative compatibility groups in fungi. Inoculum: Newsletter of the Mycological Society of America, 60(6), 1-2.
Collado-Romero, M., Mercado-Blanco, J., Olivares-García, C., & Jiménez-Díaz, R. M. (2008). Phylogenetic analysis of Verticillium dahliae vegetative compatibility groups. Phytopathology, 98(9), 1019-1028. https://doi.org/10.1094/PHYTO-98-9-1019
Correll, J. C., Klittich, C. J. R., & Leslie, J. F. (1987). Nitrate nonutilizing mutants of Fusarium oxysporum and their use in vegetative compatibility tests. Phytopathology, 77(12), 1640-1646.
Dane, E., & Demirci, E. (2012). Vegetative compatibility groups and pathogenicity of Verticillium dahliae isolates from potato plants in Erzurum province. Journal of Agricultural Sciences, 18(2), 110-114. https://doi.org/10.1501/Tarimbil_0000001198
Demirci, E., & Genc, T. (2009). Vegetative compatibility groups of Verticillium dahliae isolates from weeds in potato fields. Journal of Plant Pathology, 91(3), 671-676.

Dervis, S., Yetisir, H., Yıldırım, H., Tok, F. M., Kurt, S., & Karaca, F. (2009). Genetic and pathogenic characterization of Verticillium dahliae isolates from eggplant in Turkey. Phytoparasitica, 37, 467-476. https://doi.org/10.1007/s12600-009-0061-4
Dervis, S., Mercado-Blanco, J., Erten, L., Valverde-Corredor, A., & Pérez-Artés, E. (2010). Verticillium wilt of olive in Turkey: A survey on disease importance, pathogen diversity and susceptibility of relevant olive cultivars. European Journal of Plant Pathology, 127, 287-301. https://doi.org/10.1007/s10658-010-9595-z
Famuyini, J., Patrick, O. A., & Sedara, A. (2020). Effect of maturity stage on quality and shelf life of tomato (Lycopersicon esculentum Mill) using refrigerator storage system. Eurasian Journal of Agricultural Research, 4(1), 23-44.
Fradin, E. F., & Thomma, B. P. H. J. (2006). Physiology and molecular aspects of Verticillium wilt diseases caused by V. dahliae and V. albo-atrum. Molecular Plant Pathology, 7(2), 71-86. https://doi.org/10.1111/j.1364-3703.2006.00323.x
Fradin, E. F., Zhang, Z., Juarez Ayala, J. C., Castroverde, C. D., Nazar, R. N., Robb, J., Chun-Ming, L., & Thomma, B. P. H. J. (2009). Genetic dissection of Verticillium wilt resistance mediated by tomato Ve1. Plant Physiology, 150(1), 320-332. https://doi.org/10.1104/pp.109.136762
García-Carneros, A. B., García-Ruiz, R., & Molinero-Ruiz, L. (2014). Genetic and molecular approach to Verticillium dahliae infecting sunflower. Helia, 37(61), 205-214. https://doi.org/10.1515/helia-2014-0014
Gazozcuzade, N. (2010). Disease management in tomato production of Silifke’s plateau villages (Master thesis, Çukurova University).
Genc, T. (2012). Vegetative compatibility groups, pathogenicity and biological control of Verticillium dahliae Kleb. isolated from strawberry plants in Erzurum and Erzincan provinces (Doctoral dissertation, Atatürk University).
Genc Kesimci, T., & Demirci, E. (2020). Vegetative compatibility groups and pathogenicity of Verticillium dahliae isolates from strawberry plants in Erzurum and Erzincan provinces, Turkey. Fresenius Environmental Bulletin, 29(1), 454-462.
Genc Kesimci, T., Demirer Durak, E., & Demirci, E. (2022). Rhizoctonia species from strawberry plants in Erzincan, Turkey: Anastomosis groups and pathogenicity. Journal of Animal and Plant Sciences, 32(3), 721-728. http://doi.org/10.36899/japs.2022.3.0473
Gong, Q., Yang, Z., Wang, X., Butt, H. I., Chen, E., He, S., Zhang, C., Zhang, X., & Li, F. (2017). Salicylic acid-related cotton (Gossypium arboreum) ribosomal protein GaRPL18 contributes to resistance to Verticillium dahliae. BMC Plant Biology, 17(1), 1-15. https://doi.org/10.1186/s12870-017-1007-5
Goud, J. K., Termoshuızen, A. J., & Gams, W. (2003). Morphology of Verticillium dahliae and V. tricorpus on semi-selective media used for the detection of V. dahliae in soil. Mycological Research, 107(7), 822-830. https://doi.org/10.1017/s0953756203008050
Göre, M. E. (2007). Vegetative compatibility and pathogenicity of Verticillium dahliae isolates from the Aegean Region of Turkey. Phytoparasitica, 35(3), 222-231. https://doi.org/10.1007/BF02981154
Göre, M. E. (2009). Vegetative compatibility and pathogenicity of Verticillium dahliae isolates from Chrysanthemum in Turkey. Phytoparasitica, 37(1), 87-94. https://doi.org/10.1007/s12600-008-0001-8
Göre, M. E., Erdoğan, O., Caner, Ö. K., Aydın, M. H., & Berk, S. (2014). VCG diversity and virulence of Verticillium dahliae from commercially available cotton seed lots in Turkey. European Journal of Plant Pathology, 140(4), 689-69. https://doi.org/10.1007/s10658-014-0500-z
Hunter, D. E., Darling, H. M., Stevenson, F. J., & Cunningham, C. E. (1968). Inheritance of resistance to Verticillium wilt in Wisconsin. American Potato Journal, 45, 72-78. https://doi.org/10.1007/BF02862864
Inderbitzin, P., Bostock, R. M., Davis, R. M., Usami, T., Platt, H. W., & Subbarao, K. V. (2011). Phylogenetics and taxonomy of the fungal vascular wilt pathogen Verticillium, with the descriptions of five new species. PLoS ONE, 6(12), e28341. https://doi.org/10.1371/journal.pone.0028341
Inderbitzin, P., & Subbarao, K. V. (2014). Verticillium systematics and evolution: How confusion impedes Verticillium wilt management and how to resolve it. Phytopathology, 104(6), 564-574. https://doi.org/10.1094/phyto-11-13-0315-ia
Isaac, M. R., Leyva-Mir, S. G., Sahagun-Castellanos, J., Camara-Correia, K., Tovar-Pedraza, J. M., & Rodriguez-Perez, J. E. (2018). Occurrence, identification, and pathogenicity of Fusarium spp. associated with tomato wilt in Mexico. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46(2), 484-493. https://doi.org/10.15835/nbha46211095
Jabnoun-Khiareddine, H., Daami-Remadi, M., Hibar, K., Robb, J., & El Mahjoub, M. (2006). Effect of temperature on verticillium wilts of tomato in Tunisia. Plant Pathology Journal, 5(1), 1-6. https://doi.org/10.3923/ppj.2006.1.6
Jabnoun-Khiareddine, H., Daami-Remadi, M., Barbara, D. J., & El Mahjoub, M. (2010). Morphological variability within and among Verticillium species collected in Tunisia. Tunisian Journal of Plant Protection, 5(1), 19-38.
Jiménez-Díaz, R. M., Mercado-Blanco, J., Olivares-García, C., Collado-Romero, M., Bejarano-Alcázar, J., Rodríguez-Jurado, D., Giménez-Jaime, A., García-Jiménez, J., & Armengol, J. (2006). Genetic and virulence diversity in Verticillium dahliae populations infecting artichoke in eastern-central Spain. Phytopathology, 96(3), 288-298. https://doi.org/10.1094/PHYTO-96-0288
Jing, R., Li, H., Hu, X., Shang, W., Shen, R., Guo, C., Guo, Q., & Subbarao, K. V. (2018). Verticillium wilt caused by Verticillium dahliae and V. nonalfalfae in potato in northern China. Plant Disease, 102(10), 1958-1964. https://doi.org/10.1094/PDIS-01-18-0162-RE
Joaquim, T. R., & Rowe, R. C. (1990). Reassessment of vegetative compatibility relationships among strains of Verticillium dahliae using nitrate-nonutilizing mutants. Phytopathology, 80(11), 1160-1166. https://doi.org/10.1094/Phyto-80-1160
Joaquim, T. R., & Rowe, R. C. (1991). Vegetative compatibility and virulense of strains of Verticillium dahliae from soil and potato plants. Phytopathology, 81(5), 552-558. https://doi.org/10.1094/Phyto-81-552
Klosterman, S. J., Veronese, P., Dobinson, K. F., Subbarao, K., Heiman, D. I., Young, S., Kodira, C. D., Alvarado, L. Galagan, J. E., Birren, B., Cuomo, C., Anchieta, A., Kang, S., Gold, S. E., & Ma, L. (2008). Verticillium comparative genomics: Understanding pathogenicity and diversity. Phytopathology, 98, S185.
Klosterman, S. J., Atallah, Z. K., Vallad, G. E., & Subbarao, K. V. (2009). Diversity, pathogenicity, and management of Verticillium species. Annual Review of Phytopathology, 47, 39-62. https://doi.org/10.1146/annurev-phyto-080508-081748
Korolev, N., & Katan, T. (1997). Improved medium for selecting nitrate nonutilizing (nit) mutants of Verticillium dahliae. Phytopathology, 87(10), 1067-1070. https://doi.org/10.1094/PHYTO.1997.87.10.1067
Korolev, N., Katan, J., & Katan, T. (2000). Vegetative compatibility groups of Verticillium dahliae in Israel: Their distribution and association with pathogenicity. Phytopathology, 90(5), 529-536. https://doi.org/10.1094/PHYTO.2000.90.5.529
Kumar, S. P., Srinivasulu, A., & Babu, K. R. (2018). Symptomology of major fungal diseases on tomato and its management. Journal of Pharmacognosy and Phytochemistry, 7(6), 1817-1821.
Leslie, J. F. (1993). Fungal vegetative compatibility. Annual Review of Phytopathology, 31(1), 127-150. https://doi.org/10.1146/annurev.py.31.090193.001015
Li, J., Faino, L., Fiorin, G. L., Bashyal, S., Schaveling, A., van den Berg, G. C. M., Seidl, M. F., & Thomma, B. P. (2022). A single Verticillium dahliae effector determines pathogenicity on tomato by targeting auxin response factors. bioRxiv, 2022(11), 1-31. https://doi.org/10.1101/2022.11.22.517554
Martinelli, F., Scalenghe, R., Davino, S., Panno, S., Scuderi, G., Ruisi, P., Villa, P., Stroppiana, D., Boschetti, M., Goulart, L. R., Davis, C. E., & Dandekar, A. M. (2015). Advanced methods of plant disease detection. A review. Agronomy for Sustainable Development, 35, 1-25. https://doi.org/10.1007/s13593-014-0246-1
McDonald, B. A., & Linde, C. (2002). Pathogen population genetics, evolutionary potential, and durable resistance. Annual Review of Phytopathology, 40(1), 349-379. https://doi.org/10.1146/annurev.phyto.40.120501.101443
Panno, S., Davino, S., Caruso, A. G., Bertacca, S., Crnogorac, A., Mandić, A., Noris, E., & Matić, S. (2021). A review of the most common and economically important diseases that undermine the cultivation of tomato crop in the Mediterranean basin. Agronomy, 11(11), 2188. https://doi.org/10.3390/agronomy11112188
Papaioannou, I. A., Ligoxigakis, E. K., Vakalounakis, D. J., Markakis, E. A., & Typas, M. A. (2013). Phytopathogenic, morphological, genetic and molecular characterization of a Verticillium dahliae population from Crete, Greece. European Journal of Plant Pathology, 136(3), 577-596. https://doi.org/10.1007/s10658-013-0189-4
Pegg, G. F., & Brady, B. L. (2002). Verticillium wilts. Cabi Publishing.
Rampersad, S. N. (2010). A study of environmental factors that affect survival of pumpkin isolates of Verticillium dahliae. HortScience, 45(8), 1211-1217. https://doi.org/10.21273/HORTSCI.45.8.1211
Retief, E., Lamprecht, S., & McLeod, A. (2023). Characterisation and pathogenicity of Verticillium dahliae isolates associated with Verticillium wilt of tomato in the Limpopo Province of South Africa. Journal of Plant Pathology, 105(4), 1465-1481. https://doi.org/10.1007/s42161-023-01446-6
Sanei, S. J., Waliyar, F., Razavi, S. I., & Okhovvat, S. M. (2008). Vegetative compatibility, host range and pathogenicity of Verticillium dahliae isolates in Iran. International Journal of Plant Production, 2(1), 37-46. https://doi.org/10.22069/IJPP.2012.598
Schreinemachers, P., Simmons, E. B., & Wopereis, M. C. (2018). Tapping the economic and nutritional power of vegetables. Global Food Security, 16, 36-45. https://doi.org/10.1016/j.gfs.2017.09.005
Senthamilselvi, S., & Victoria, J. (2019). An overview of wilt diseases of tomato. International Journal of Pharmacy and Biological Sciences, 9(3), 360-369. https://doi.org/10.21276/ijpbs.2019.9.3.47
Sunpapao, A., Suwannarach, N., Kumla, J., Dumhai, R., Riangwong, K., Sanguansub, S., Wanchana, S., & Arikit, S. (2022). Morphological and molecular identification of plant pathogenic fungi associated with dirty panicle disease in coconuts (Cocos nucifera) in Thailand. Journal of Fungi, 8(4), 335. https://doi.org/10.3390/jof8040335
Tjamos, E. C. (1981). Virulence of Verticillium dahliae and V. albo-atrum isolates in tomato seedlings in relation to their host of origin and the applied cropping system. Phytopathology, 71, 98-100. https://doi.org/10.1094/Phyto-71-98
Tsror, L., Hazanovsky, M., Mordechi‐Lebiush, S., & Sivan, S. (2001). Aggressiveness of Verticillium dahliae isolates from different vegetative compatibility groups to potato and tomato. Plant Pathology, 50(4), 477-482. https://doi.org/10.1046/j.1365-3059.2001.00587.x
White, T. J., Bruns, T., Lee, S. J. W. T., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. White (Eds.), PCR protocols: A guide to methods and applications (pp. 315-322). Academic Press.
Yucel, S., Can, C., Yurtmen, M., Cetinkaya-Yildiz, R., & Aysan, Y. (2008). Tomato pathology in Turkey. The European Journal of Plant Science and Biotechnology, 2(1), 38-47.
Zeise, K., & Von Tiedemann, A. (2002). Application of RAPD‐PCR for virulence type analysis within Verticillium dahliae and V. longisporum. Journal of Phytopathology, 150(10), 557-563. https://doi.org/10.1046/j.1439-0434.2002.00799.x
Zhen, X. H., & Li, Y. Z. (2004). Ultrastructural changes and location of β-1, 3-glucanase in resistant and susceptible cotton callus cells in response to treatment with toxin of Verticillium dahliae and salicylic acid. Journal of Plant Physiology, 161(12), 1367-1377. https://doi.org/10.1016/j.jplph.2004.04.007

Details

Primary Language

English

Subjects

Phytopathology

Journal Section

Research Article

Authors

Tuba Genç Kesimci ^*
0000-0003-2022-0193
Türkiye

Publication Date

June 22, 2024

Submission Date

February 8, 2024

Acceptance Date

April 29, 2024

Published in Issue

Year 2024 Volume: 5 Number: 2

DOI

https://doi.org/10.56430/japro.1433931

IZ

https://izlik.org/JA62AN84JT

APA

Genç Kesimci, T. (2024). Determination and Pathogenicity of Verticillium dahliae Isolates Obtained from Tomato Plants (Solanum lycopersicum L.) in the Iğdır Province. Journal of Agricultural Production, 5(2), 81-90. https://doi.org/10.56430/japro.1433931

AMA

1.Genç Kesimci T. Determination and Pathogenicity of Verticillium dahliae Isolates Obtained from Tomato Plants (Solanum lycopersicum L.) in the Iğdır Province. J Agri Pro. 2024;5(2):81-90. doi:10.56430/japro.1433931

Chicago

Genç Kesimci, Tuba. 2024. “Determination and Pathogenicity of Verticillium Dahliae Isolates Obtained from Tomato Plants (Solanum Lycopersicum L.) in the Iğdır Province”. Journal of Agricultural Production 5 (2): 81-90. https://doi.org/10.56430/japro.1433931.

EndNote

Genç Kesimci T (June 1, 2024) Determination and Pathogenicity of Verticillium dahliae Isolates Obtained from Tomato Plants (Solanum lycopersicum L.) in the Iğdır Province. Journal of Agricultural Production 5 2 81–90.

IEEE

[1]T. Genç Kesimci, “Determination and Pathogenicity of Verticillium dahliae Isolates Obtained from Tomato Plants (Solanum lycopersicum L.) in the Iğdır Province”, J Agri Pro, vol. 5, no. 2, pp. 81–90, June 2024, doi: 10.56430/japro.1433931.

ISNAD

Genç Kesimci, Tuba. “Determination and Pathogenicity of Verticillium Dahliae Isolates Obtained from Tomato Plants (Solanum Lycopersicum L.) in the Iğdır Province”. Journal of Agricultural Production 5/2 (June 1, 2024): 81-90. https://doi.org/10.56430/japro.1433931.

JAMA

1.Genç Kesimci T. Determination and Pathogenicity of Verticillium dahliae Isolates Obtained from Tomato Plants (Solanum lycopersicum L.) in the Iğdır Province. J Agri Pro. 2024;5:81–90.

MLA

Genç Kesimci, Tuba. “Determination and Pathogenicity of Verticillium Dahliae Isolates Obtained from Tomato Plants (Solanum Lycopersicum L.) in the Iğdır Province”. Journal of Agricultural Production, vol. 5, no. 2, June 2024, pp. 81-90, doi:10.56430/japro.1433931.

Vancouver

1.Tuba Genç Kesimci. Determination and Pathogenicity of Verticillium dahliae Isolates Obtained from Tomato Plants (Solanum lycopersicum L.) in the Iğdır Province. J Agri Pro. 2024 Jun. 1;5(2):81-90. doi:10.56430/japro.1433931

Abstract

Determination and Pathogenicity of Verticillium dahliae Isolates Obtained from Tomato Plants (Solanum lycopersicum L.) in the Iğdır Province

Abstract

Keywords

Supporting Institution

Project Number

Ethical Statement

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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