Genome-Wide Analysis of Vacuolar Iron Transporter (VIT) Gene Family in Phaseolus vulgaris L.: Functional Roles in Heavy Metal Stress

Esma Yigider

doi:10.56430/japro.1593604

EN

Genome-Wide Analysis of Vacuolar Iron Transporter (VIT) Gene Family in Phaseolus vulgaris L.: Functional Roles in Heavy Metal Stress

Abstract

Vacuolar Iron Transporter (VIT) genes have been characterized and indicated to play critical roles in iron homeostasis in various plants. Heavy metals pose a significant challenge to bean cultivation, necessitating the development of heavy metal-resistant cultivars as a key strategy to mitigate their impacts. Vacuolar detoxification is a crucial strategy for plants to survive and adapt to the adverse environment caused by heavy metal stress. The current study used various bioinformatic tools to characterize the VIT gene in the bean, a significant member of the legume family and an important agricultural product, for the first time. The study identified and characterized 11 VIT genes (PhvulVIT-1–PhvulVIT-11) in the bean's genome. These genes displayed molecular weights (MW) ranging from 16.48 to 28.92 kDa and comprised 155–269 amino acid residues. The distribution of the 11 PhvulVIT genes on the four chromosomes was not homogeneous, and eight genes were observed to be located on chromosome 2. Gene duplication events suggested purifying selection as the primary evolutionary force, ensuring functional stability of duplicated genes. Phylogenetic analysis classified PhvulVIT genes into three clades, reflecting evolutionary relationships with orthologs in Arabidopsis thaliana and Glycine max. Cis-regulatory element analysis of promoter regions revealed key stress-responsive motifs like MYB, MYC, and ABRE, which are essential for plant responses to environmental stresses and phytohormone signaling. Additionally, the expression patterns of PhvulVIT under heavy metal conditions were examined using RNAseq. This study enhances our understanding of the functional roles of VIT genes in nutrient homeostasis and environmental stress adaptation, offering valuable insights for crop improvement strategies, including biofortification and the development of stress-tolerant cultivars.

Keywords

Ccc1 domain , Iron transport , RNAseq , Vacuole , VTL

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Details

Primary Language

English

Subjects

Plant Biotechnology in Agriculture

Journal Section

Research Article

Authors

Esma Yigider ^*
0000-0002-6896-0193
Türkiye

Publication Date

March 26, 2025

Submission Date

November 29, 2024

Acceptance Date

February 3, 2025

Published in Issue

Year 2025 Volume: 6 Number: 1

DOI

https://doi.org/10.56430/japro.1593604

IZ

https://izlik.org/JA78WE96AH

APA

Yigider, E. (2025). Genome-Wide Analysis of Vacuolar Iron Transporter (VIT) Gene Family in Phaseolus vulgaris L.: Functional Roles in Heavy Metal Stress. Journal of Agricultural Production, 6(1), 15-31. https://doi.org/10.56430/japro.1593604

Genome-Wide Analysis of Vacuolar Iron Transporter (VIT) Gene Family in Phaseolus vulgaris L.: Functional Roles in Heavy Metal Stress

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Genome-Wide Analysis of Vacuolar Iron Transporter (VIT) Gene Family in Phaseolus vulgaris L.: Functional Roles in Heavy Metal Stress

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Submission Date

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