Protein-Level Assessment of Aquaporins in Alfalfa Under Salt Stress

Dilara Kaynar; Özgür Kaynar

doi:10.56430/japro.1806878

EN

Protein-Level Assessment of Aquaporins in Alfalfa Under Salt Stress

Abstract

Aquaporins (AQPs) are integral membrane proteins that facilitate water and solute transport, playing crucial roles in stress adaptation in both plants and animals. In mammals, aquaglyceroporins such as AQP3 and AQP7 are well characterized, whereas their plant counterparts remain less understood, particularly at the protein level. In this study, alfalfa (Medicago sativa) was grown in Hoagland’s medium supplemented with 0, 50, 100, 200, or 400 mM NaCl for 21 days. Protein extracts were analyzed by SDS-PAGE and Western blotting using antibodies against human - mouse - rat AQP3 and mouse - human AQP7. Two distinct cross-reactive protein bands were identified. Their expression was weak at 200 mM NaCl but strongly induced under severe salt stress at 400 mM NaCl. These results suggest a threshold-dependent regulation of aquaporin-like proteins in alfalfa. Unlike transcriptomic analyses, our protein-level approach highlights stress-specific expression dynamics and points to potential functional similarities with mammalian aquaglyceroporins, particularly in osmolyte transport and osmotic adjustment. This study underscores the importance of direct protein analysis for understanding stress physiology and provides new insights into salt stress adaptation mechanisms in forage legumes.

Keywords

Alfalfa, Aquaglyceroporins, Aquaporins, Protein expression, Salt stress

Ethical Statement

This study does not require ethical committee approval.

Thanks

A part of this article was presented as an abstract at the 6th International Congress on Engineering and Life Science held in Kyrenia between 2-4 September, 2025.

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Details

Primary Language

English

Subjects

Crop and Pasture Biomass and Bioproducts

Journal Section

Research Article

Authors

Dilara Kaynar ^*
0000-0002-8946-3217
Türkiye

Özgür Kaynar
0000-0002-2875-423X
Türkiye

Publication Date

June 30, 2026

Submission Date

October 19, 2025

Acceptance Date

March 24, 2026

Published in Issue

Year 2026 Volume: 7 Number: 2

DOI

https://doi.org/10.56430/japro.1806878

IZ

https://izlik.org/JA55TL73YU

APA

Kaynar, D., & Kaynar, Ö. (2026). Protein-Level Assessment of Aquaporins in Alfalfa Under Salt Stress. Journal of Agricultural Production, 7(2), 97-102. https://doi.org/10.56430/japro.1806878

AMA

1.Kaynar D, Kaynar Ö. Protein-Level Assessment of Aquaporins in Alfalfa Under Salt Stress. J Agri Pro. 2026;7(2):97-102. doi:10.56430/japro.1806878

Chicago

Kaynar, Dilara, and Özgür Kaynar. 2026. “Protein-Level Assessment of Aquaporins in Alfalfa Under Salt Stress”. Journal of Agricultural Production 7 (2): 97-102. https://doi.org/10.56430/japro.1806878.

EndNote

Kaynar D, Kaynar Ö (June 1, 2026) Protein-Level Assessment of Aquaporins in Alfalfa Under Salt Stress. Journal of Agricultural Production 7 2 97–102.

IEEE

[1]D. Kaynar and Ö. Kaynar, “Protein-Level Assessment of Aquaporins in Alfalfa Under Salt Stress”, J Agri Pro, vol. 7, no. 2, pp. 97–102, June 2026, doi: 10.56430/japro.1806878.

ISNAD

Kaynar, Dilara - Kaynar, Özgür. “Protein-Level Assessment of Aquaporins in Alfalfa Under Salt Stress”. Journal of Agricultural Production 7/2 (June 1, 2026): 97-102. https://doi.org/10.56430/japro.1806878.

JAMA

1.Kaynar D, Kaynar Ö. Protein-Level Assessment of Aquaporins in Alfalfa Under Salt Stress. J Agri Pro. 2026;7:97–102.

MLA

Kaynar, Dilara, and Özgür Kaynar. “Protein-Level Assessment of Aquaporins in Alfalfa Under Salt Stress”. Journal of Agricultural Production, vol. 7, no. 2, June 2026, pp. 97-102, doi:10.56430/japro.1806878.

Vancouver

1.Dilara Kaynar, Özgür Kaynar. Protein-Level Assessment of Aquaporins in Alfalfa Under Salt Stress. J Agri Pro. 2026 Jun. 1;7(2):97-102. doi:10.56430/japro.1806878

Protein-Level Assessment of Aquaporins in Alfalfa Under Salt Stress

Abstract

Keywords

Ethical Statement

Thanks

Abstract

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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