In vitro Effect of Bacterial Biocontrol Organisms against Pectobacterium carotovorum on Potato

Year 2020, Volume: 1 Issue: 1, 8 - 11, 31.12.2020

Fatih Dadaşoğlu John Fredy Maldonado Joy Gülsüm Özyurt Recep Kotan

Abstract

Soft rot is a disease that cause substantial economic losses in potato production. Pectobacterium carotovorum is known as one of the most common soft rot causative agents and the disease has not effective control methods being developed yet. In this study the effect of 3 different bacterial isolates of Bacillus megaterium (B60d, TV6D, TV91C), 2 of Paenibacillus polymyxa (Ç9, TV12E), 2 of Bacillus subtilis (TV6F, TV17C), 1 of Pantoea agglomerans (B79), 1 of Agrobacterium radiobacter (A16), 1 of Bacillus megaterium gc. subgroup A (FDG161), 1 of Bacillus atrophaeus (FD1), 1 of Pseudomonas fluorescens biotype F (FDG37) and 1 of Bacillus pumilus (TV3D) were tested against 5 pathogenic bacterial strains of P. carotovorum subsp. carotovorum (F37, F680, F741, F331, F742), using dual culture method in in vitro conditions. The most effective bacterial isolate against strains Pectobacterium were P. carotovorum F37 + Bacillus megaterium B60d (55,00 mm) isolate, followed by P. carotovorum F680 + Bacillus megaterium B60d (44,00 mm) and P. carotovorum F742 + Bacillus megaterium B60d (39,33 mm) isolates. Therefore, Bacillus megaterium has a promising biocontrol activity against tested plant pathogenic bacteria under in vitro conditions.

Keywords

MIS, BIOLOG, Pectolytic activity, Soft rot, Pectobacterium carotovorum

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