Research Article
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Mycoflora of Stored Wheat in Bafra District of Samsun Province

Year 2024, Volume: 5 Issue: 4, 234 - 240, 31.12.2024
https://doi.org/10.56430/japro.1484041

Abstract

In this study, the fungal flora in wheat grain samples taken six months after harvest from warehouses in 13 villages of Bafra district of Samsun province was investigated. A total of 600 seeds were processed isolates of endophytic, saprophytic, or pathogenic fungi recovered were identified as 15 fungal genera. Alternaria alternata, Alternaria spp., Chaetomium spp., Phoma spp., Epicoccum nigrum were the fungi that showed the highest colonization frequency in analyzed grain. Fungi such as Penicillium, Aspergillus, Fusarium graminearum, F. poae, which are known to produce mycotoxins, were among the isolated fungi. Fusarium graminearum, F. poae and Bipolaris sorokiniana are among the important pathogens of wheat. The other microorganisms were present at intermediate or low values. On the other hand, fungi such as Chaetomium, Epicoccum nigrum, Torula species were isolated as antagonist organisms. Stemphylum, Ulocladium, Cladosporium, Popularia, Nigrospora oryzae, which are thought to be saprophytes or endophytes, were also isolated. Some are also known as weak pathogens. On average, 31.5% of the seeds examined had one or more fungal infections, while 68.5% had no fungal infections.

References

  • Andrew, M., Peever, T. L., & Pryor, B. M. (2009). An expanded multilocus phylogeny does not resolve morphological species within the small-spored Alternaria species complex. Mycologia, 101(1), 95-109. https://doi.org/10.3852/08-135
  • Barkai-Golan, R. (2008). Alternaria mycotoxins. In R. Barkai-Golan & N. Paster (Eds.), Mycotoxins in fruits and vegetables (pp. 185-203). Elsevier. https://doi.org/10.1016/B978-0-12-374126-4.00008-5
  • Barnett, H. L. & Hunter, B. B. (1998). Illustrated genera of imperfect fungi. APS Press.
  • Berestetskly, A. O. (2008). A review of fungal phytotoxins: From basic studies to practical use. Applied Biochemical and Microbiology, 44, 453-465. https://doi.org/10.1134/S0003683808050013
  • Booth, C. (1977). Fusarium. Laboratory guide to the identification of the major species. Commenwealth Mycology Institute.
  • Bottalico, A., & Logrieco, A. (1998). Alternaria species of economic importance. In K. K. Sinha & D. Bhatnagar (Eds.), Mycotoxins in agriculture and food safety (pp. 65-108). Marcel Dekker.
  • Burgess, L. W., Summerell, B. A., Bullock, S., Gott, K. P., & Backhouse, D. (1994). Laboratory manual for Fusarium research. Fusarium Research Laboratory Department of Crop Sciences University of Sydney and Royal Botanic Gardens.
  • Duncan, D. B. (1955). Multiple range and multiple F tests. Biometrics, 11(1), 1-42. https://doi.org/10.2307/3001478
  • EFSA. (2011). Scientific opinion on the risks for animal and public health related to the presence of Alternaria toxins in feed and food. EFSA Journal, 9(10) 2407. https://doi.org/10.2903/j.efsa.2011.2407
  • Erdem, H., & Özen, N. (1990). Aflatoksinlerin insan ve hayvan sağlığı açısından önemi. Ondokuz Mayıs Üniversitesi Ziraat fakültesi Dergisi, 5(1-2). (In Turkish)
  • Ertugay, Z. (2010). Buğday, un ve ekmek arasındaki kalite ilişkileri. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 13(1-2), 165-176. (In Turkish)
  • Evren, M. (1999). Aflatoksinlerin etki şekilleri, gıdalarda bulunma durumları ve önleme çareleri. Ondokuz Mayıs Üniversitesi Ziraat Fakültesi Dergisi, 14(2), 159-172. (In Turkish)
  • Friesen, T. L., Faris, J. D., Solomon, P. S., & Oliver, R. P. (2008). Host-specific toxins: Effectors of necrotrophic pathogenicity. Cell Microbiology, 10(7), 1421-1428. https://doi.org/10.1111/j.1462-5822.2008.01153.x
  • Ghosh, J., Nandi, B., & Fries, N. (1981). Deterioration of stored wheat caused by fungal infections under different conditions of temperature and relative humidity. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz/Journal of Plant Diseases and Protection, 88, 9-17.
  • Hamayun, M., Khan, S. A., Khan, A. L., Rehman, G., Kim, Y. H., Iqbal, I., Hussain, J. & Lee, I. J. (2010). Gibberellin production and plant growth promotion from pure cultures of Cladosporium sp. MH-6 isolated from cucumber (Cucumis sativus L.). Mycologia, 102(5), 989-995. https://doi.org/10.3852/09-261
  • Kawaide, H. (2006). Biochemical and molecular analyses of gibberellin biosynthesis in fungi. Bioscience, Biotechnology, and Biochemistry, 70(3), 583-590. https://doi.org/10.1271/bbb.70.583
  • Larran, S., Perello, A., Simon M. R., & Moreno, V. (2002). Isolation and analysis of endophytic microorganisms in wheat (Triticum aestivum L.) leaves. World Journal of Microbiology & Biotechnology, 18, 683-686, 2002. https://doi.org/10.1023/A:1016857917950
  • Larran, S., Perello, A., Simon M. R., & Moreno, V. (2007). The endophytic fungi from wheat (Triticum aestivum L.). World Journal of Microbiology & Biotechnology, 23, 565-572. https://doi.org/10.1007/s11274-006-9266-6
  • Levene, H. (1960). Robust tests for equality of variances. In I. Olkin (Ed.), Contributions to probability and statistics (pp. 278-292). Stanford University Press.
  • Logrieco, A., Moretti, A., & Solfrizzo, M. (2009). Alternaria toxins and plant diseases: An overview of origin, occurence and risks. World Mycotoxin Journal, 2(2), 129-140. https://doi.org/10.3920/WMJ2009.1145
  • MacMillan, C. P., Blundell, C. A., & King, R. W. (2005). Flowering of the grass Lolium perenne. Effects of vernalization and long days on gibberellin biosynthesis and signaling. Plant Physiology, 138(3), 1794-1806. https://doi.org/10.1104/pp.105.062190
  • Magan, N., Medina, A., & Aldred, D. (2011). Possible climate-change effects on mycotoxin contamination of food crops pre- and postharvest. Plant Pathology, 60(1), 150-163. https://doi.org/10.1111/j.1365-3059.2010.02412.x
  • Malinowski, D. P., & Belesky, D. P. (1999). Neotyphodium coenophialum‐endophyte infection affects the ability of tall fescue to use sparingly available phosphorus. Journal of Plant Nutrition, 22(4-5), 835-853. https://doi.org/10.1080/01904169909365675
  • Muir, W. E. (1980). Grain drying and storage in Canada. The Society of Agricultural Structures, Japan, 10(2), 91-105.
  • Munkvold, G. P., Proctor, R. H., & Moretti, A. (2021). Mycotoxin production in Fusarium according to contemporary species concepts. Annual Review of Phytopathology, 59, 373-402. https://doi.org/10.1146/annurev-phyto-020620-102825
  • Nelson, P. E., Toussoun, T. S., & Marasas, W. F. O. (1983). Fusarium species: An illustrated manual for identification. Pennsylvania State University Press.
  • Olgun, M. (2011). Tarımsal yapılar. Ankara Üniversitesi Ziraat Fakültesi Yayınları. (In Turkish)
  • Pinto, V. E. F., & Patriarca, A. (2017). Alternaria species and their associated mycotoxins. In A. Moretti & A. Susca (Eds.), Mycotoxigenic fungi: Methods and protocols (pp. 13-32). Springer. https://doi.org/10.1007/978-1-4939-6707-0_2
  • Placinta, C. M., D’Mello, C. P. F., & MacDonald, A. M. C. (1999). A review of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins. Animal Feed Science and Technology, 78(1-2), 21-37. https://doi.org/10.1016/s0377-8401(98)00278-8
  • Prasher, P., Sharma, M., & Kumar, T. (2024). Chapter 6: Biocontrol of Aspergillus and Penicillium mycotoxins: Benefits and limitations. In K. A. Abd-Elsalam & H. I. Mohamed (Eds.), Fungal secondary metabolites: Synthesis and applications in agroecosystem; a volume in nanobiotechnology for plant protection (pp. 117-129). Elsevier. https://doi.org/10.1016/B978-0-323-95241-5.00021-6
  • Reis, V. M., Dos Reis, J., Fabio, B., Salles, J. F., & Schloter, M. (2000). Characterisation of different polyclonal antisera to quantify Herbaspirillum spp. in elephant grass (Pennisetum purpureum Schun.). Symbiosis, 29(2), 139-150.
  • Shiferaw, B., Smale, M., Braun, H. J., Duveiller, E., Reynolds, M., & Muricho, G. (2013). Crops that feed the world 10. Past successes and future challenges to the role played by wheat in global food security. Food Security, 5, 291-317. https://doi.org/10.1007/s12571-013-0263-y
  • Solanki, M. K., Droby, S., & Sionov, E. (2021). The wheat microbiome in relation to Mycotoxin occurrence in stored grain: An overview. In D. Spadaro, S. Droby & M. L. Gullino (Eds.), Postharvest pathology: Next generation solutions to reducing losses and enhancing safety (pp. 129-139). Springer. https://doi.org/10.1007/978-3-030-56530-5_8
  • Tan, R. X., & Zou, W. X. (2001). Endophytes: A rich source of functional metabolites. Natural Product Reports, 18(4), 448-459. https://doi.org/10.1039/b100918o
  • Timm, N. S., Lang, G. H., Ramos, A. H., Pohndorf, R. S., Ferreira, C. D., & Oliveira, M. (2020). Effects of drying methods and temperatures on protein, pasting, and thermal properties of white floury corn. Journal of Food Processing and Preservation, 44(10), e14767. https://doi.org/10.1111/jfpp.14767
  • Tunalı, B., Küçüktopçu, Y., Tunalı, N., Erken Meral, S., Eker, S., & Kansu, B. (2023). Alternaria mikotoksinleri ve önemi. Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 37(1), 195-219. https://doi.org/10.20479/bursauludagziraat.1111062 (In Turkish)
  • Ünlütürk, A., & Turantaş, F. (1998). Gıda mikrobiyolojisi. Ege Üniversitesi Yayınları. (In Turkish)
  • Vandenbussche, F., Fierro, A. C., Wiedemann, G., Reski, R., & Van Der Straeten, D. (2007). Evolutionary conservation of plant gibberellin signalling pathway components. BMC Plant Biology, 7, 1-17. https://doi.org/10.1186/1471-2229-7-65
  • Vesonder, R. F., Ciegler, A., & Jensen, A. H. (1973). Isolation of emetic principle from Fusarium-infected corn. Applied Microbiology, 26(6), 1008-1010. https://doi.org/10.1128/am.26.6.1008-1010.1973
  • Wallace, H. A. H., Sholberg, P. L., Sinha, R. N., & Muir, W. E. (1983). Biological, physical and chemical changes in stored wheat. Mycopathologia, 82, 65-76. https://doi.org/10.1007/BF00437333
  • Wolter, K. (1986). Flour quality flour storage flour temperature. Allgemeine Baecker Zeitung, 41(3-49, 6.
  • Yoshizawa, T., & Morooka, N. (1973). Deoxynivalenol and its monoacetate: New mycotoxins from Fusarium roseum and moldy barley. Agricultural and Biological Chemistry, 37(12), 2933-2934. https://doi.org/10.1080/00021369.1973.10861103
Year 2024, Volume: 5 Issue: 4, 234 - 240, 31.12.2024
https://doi.org/10.56430/japro.1484041

Abstract

References

  • Andrew, M., Peever, T. L., & Pryor, B. M. (2009). An expanded multilocus phylogeny does not resolve morphological species within the small-spored Alternaria species complex. Mycologia, 101(1), 95-109. https://doi.org/10.3852/08-135
  • Barkai-Golan, R. (2008). Alternaria mycotoxins. In R. Barkai-Golan & N. Paster (Eds.), Mycotoxins in fruits and vegetables (pp. 185-203). Elsevier. https://doi.org/10.1016/B978-0-12-374126-4.00008-5
  • Barnett, H. L. & Hunter, B. B. (1998). Illustrated genera of imperfect fungi. APS Press.
  • Berestetskly, A. O. (2008). A review of fungal phytotoxins: From basic studies to practical use. Applied Biochemical and Microbiology, 44, 453-465. https://doi.org/10.1134/S0003683808050013
  • Booth, C. (1977). Fusarium. Laboratory guide to the identification of the major species. Commenwealth Mycology Institute.
  • Bottalico, A., & Logrieco, A. (1998). Alternaria species of economic importance. In K. K. Sinha & D. Bhatnagar (Eds.), Mycotoxins in agriculture and food safety (pp. 65-108). Marcel Dekker.
  • Burgess, L. W., Summerell, B. A., Bullock, S., Gott, K. P., & Backhouse, D. (1994). Laboratory manual for Fusarium research. Fusarium Research Laboratory Department of Crop Sciences University of Sydney and Royal Botanic Gardens.
  • Duncan, D. B. (1955). Multiple range and multiple F tests. Biometrics, 11(1), 1-42. https://doi.org/10.2307/3001478
  • EFSA. (2011). Scientific opinion on the risks for animal and public health related to the presence of Alternaria toxins in feed and food. EFSA Journal, 9(10) 2407. https://doi.org/10.2903/j.efsa.2011.2407
  • Erdem, H., & Özen, N. (1990). Aflatoksinlerin insan ve hayvan sağlığı açısından önemi. Ondokuz Mayıs Üniversitesi Ziraat fakültesi Dergisi, 5(1-2). (In Turkish)
  • Ertugay, Z. (2010). Buğday, un ve ekmek arasındaki kalite ilişkileri. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 13(1-2), 165-176. (In Turkish)
  • Evren, M. (1999). Aflatoksinlerin etki şekilleri, gıdalarda bulunma durumları ve önleme çareleri. Ondokuz Mayıs Üniversitesi Ziraat Fakültesi Dergisi, 14(2), 159-172. (In Turkish)
  • Friesen, T. L., Faris, J. D., Solomon, P. S., & Oliver, R. P. (2008). Host-specific toxins: Effectors of necrotrophic pathogenicity. Cell Microbiology, 10(7), 1421-1428. https://doi.org/10.1111/j.1462-5822.2008.01153.x
  • Ghosh, J., Nandi, B., & Fries, N. (1981). Deterioration of stored wheat caused by fungal infections under different conditions of temperature and relative humidity. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz/Journal of Plant Diseases and Protection, 88, 9-17.
  • Hamayun, M., Khan, S. A., Khan, A. L., Rehman, G., Kim, Y. H., Iqbal, I., Hussain, J. & Lee, I. J. (2010). Gibberellin production and plant growth promotion from pure cultures of Cladosporium sp. MH-6 isolated from cucumber (Cucumis sativus L.). Mycologia, 102(5), 989-995. https://doi.org/10.3852/09-261
  • Kawaide, H. (2006). Biochemical and molecular analyses of gibberellin biosynthesis in fungi. Bioscience, Biotechnology, and Biochemistry, 70(3), 583-590. https://doi.org/10.1271/bbb.70.583
  • Larran, S., Perello, A., Simon M. R., & Moreno, V. (2002). Isolation and analysis of endophytic microorganisms in wheat (Triticum aestivum L.) leaves. World Journal of Microbiology & Biotechnology, 18, 683-686, 2002. https://doi.org/10.1023/A:1016857917950
  • Larran, S., Perello, A., Simon M. R., & Moreno, V. (2007). The endophytic fungi from wheat (Triticum aestivum L.). World Journal of Microbiology & Biotechnology, 23, 565-572. https://doi.org/10.1007/s11274-006-9266-6
  • Levene, H. (1960). Robust tests for equality of variances. In I. Olkin (Ed.), Contributions to probability and statistics (pp. 278-292). Stanford University Press.
  • Logrieco, A., Moretti, A., & Solfrizzo, M. (2009). Alternaria toxins and plant diseases: An overview of origin, occurence and risks. World Mycotoxin Journal, 2(2), 129-140. https://doi.org/10.3920/WMJ2009.1145
  • MacMillan, C. P., Blundell, C. A., & King, R. W. (2005). Flowering of the grass Lolium perenne. Effects of vernalization and long days on gibberellin biosynthesis and signaling. Plant Physiology, 138(3), 1794-1806. https://doi.org/10.1104/pp.105.062190
  • Magan, N., Medina, A., & Aldred, D. (2011). Possible climate-change effects on mycotoxin contamination of food crops pre- and postharvest. Plant Pathology, 60(1), 150-163. https://doi.org/10.1111/j.1365-3059.2010.02412.x
  • Malinowski, D. P., & Belesky, D. P. (1999). Neotyphodium coenophialum‐endophyte infection affects the ability of tall fescue to use sparingly available phosphorus. Journal of Plant Nutrition, 22(4-5), 835-853. https://doi.org/10.1080/01904169909365675
  • Muir, W. E. (1980). Grain drying and storage in Canada. The Society of Agricultural Structures, Japan, 10(2), 91-105.
  • Munkvold, G. P., Proctor, R. H., & Moretti, A. (2021). Mycotoxin production in Fusarium according to contemporary species concepts. Annual Review of Phytopathology, 59, 373-402. https://doi.org/10.1146/annurev-phyto-020620-102825
  • Nelson, P. E., Toussoun, T. S., & Marasas, W. F. O. (1983). Fusarium species: An illustrated manual for identification. Pennsylvania State University Press.
  • Olgun, M. (2011). Tarımsal yapılar. Ankara Üniversitesi Ziraat Fakültesi Yayınları. (In Turkish)
  • Pinto, V. E. F., & Patriarca, A. (2017). Alternaria species and their associated mycotoxins. In A. Moretti & A. Susca (Eds.), Mycotoxigenic fungi: Methods and protocols (pp. 13-32). Springer. https://doi.org/10.1007/978-1-4939-6707-0_2
  • Placinta, C. M., D’Mello, C. P. F., & MacDonald, A. M. C. (1999). A review of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins. Animal Feed Science and Technology, 78(1-2), 21-37. https://doi.org/10.1016/s0377-8401(98)00278-8
  • Prasher, P., Sharma, M., & Kumar, T. (2024). Chapter 6: Biocontrol of Aspergillus and Penicillium mycotoxins: Benefits and limitations. In K. A. Abd-Elsalam & H. I. Mohamed (Eds.), Fungal secondary metabolites: Synthesis and applications in agroecosystem; a volume in nanobiotechnology for plant protection (pp. 117-129). Elsevier. https://doi.org/10.1016/B978-0-323-95241-5.00021-6
  • Reis, V. M., Dos Reis, J., Fabio, B., Salles, J. F., & Schloter, M. (2000). Characterisation of different polyclonal antisera to quantify Herbaspirillum spp. in elephant grass (Pennisetum purpureum Schun.). Symbiosis, 29(2), 139-150.
  • Shiferaw, B., Smale, M., Braun, H. J., Duveiller, E., Reynolds, M., & Muricho, G. (2013). Crops that feed the world 10. Past successes and future challenges to the role played by wheat in global food security. Food Security, 5, 291-317. https://doi.org/10.1007/s12571-013-0263-y
  • Solanki, M. K., Droby, S., & Sionov, E. (2021). The wheat microbiome in relation to Mycotoxin occurrence in stored grain: An overview. In D. Spadaro, S. Droby & M. L. Gullino (Eds.), Postharvest pathology: Next generation solutions to reducing losses and enhancing safety (pp. 129-139). Springer. https://doi.org/10.1007/978-3-030-56530-5_8
  • Tan, R. X., & Zou, W. X. (2001). Endophytes: A rich source of functional metabolites. Natural Product Reports, 18(4), 448-459. https://doi.org/10.1039/b100918o
  • Timm, N. S., Lang, G. H., Ramos, A. H., Pohndorf, R. S., Ferreira, C. D., & Oliveira, M. (2020). Effects of drying methods and temperatures on protein, pasting, and thermal properties of white floury corn. Journal of Food Processing and Preservation, 44(10), e14767. https://doi.org/10.1111/jfpp.14767
  • Tunalı, B., Küçüktopçu, Y., Tunalı, N., Erken Meral, S., Eker, S., & Kansu, B. (2023). Alternaria mikotoksinleri ve önemi. Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 37(1), 195-219. https://doi.org/10.20479/bursauludagziraat.1111062 (In Turkish)
  • Ünlütürk, A., & Turantaş, F. (1998). Gıda mikrobiyolojisi. Ege Üniversitesi Yayınları. (In Turkish)
  • Vandenbussche, F., Fierro, A. C., Wiedemann, G., Reski, R., & Van Der Straeten, D. (2007). Evolutionary conservation of plant gibberellin signalling pathway components. BMC Plant Biology, 7, 1-17. https://doi.org/10.1186/1471-2229-7-65
  • Vesonder, R. F., Ciegler, A., & Jensen, A. H. (1973). Isolation of emetic principle from Fusarium-infected corn. Applied Microbiology, 26(6), 1008-1010. https://doi.org/10.1128/am.26.6.1008-1010.1973
  • Wallace, H. A. H., Sholberg, P. L., Sinha, R. N., & Muir, W. E. (1983). Biological, physical and chemical changes in stored wheat. Mycopathologia, 82, 65-76. https://doi.org/10.1007/BF00437333
  • Wolter, K. (1986). Flour quality flour storage flour temperature. Allgemeine Baecker Zeitung, 41(3-49, 6.
  • Yoshizawa, T., & Morooka, N. (1973). Deoxynivalenol and its monoacetate: New mycotoxins from Fusarium roseum and moldy barley. Agricultural and Biological Chemistry, 37(12), 2933-2934. https://doi.org/10.1080/00021369.1973.10861103
There are 42 citations in total.

Details

Primary Language English
Subjects Phytopathology
Journal Section Research Articles
Authors

Ümit Eser 0000-0002-5023-0811

Bayram Kansu 0000-0001-5663-0528

Berna Tunalı 0000-0003-2798-0777

Publication Date December 31, 2024
Submission Date May 14, 2024
Acceptance Date October 16, 2024
Published in Issue Year 2024 Volume: 5 Issue: 4

Cite

APA Eser, Ü., Kansu, B., & Tunalı, B. (2024). Mycoflora of Stored Wheat in Bafra District of Samsun Province. Journal of Agricultural Production, 5(4), 234-240. https://doi.org/10.56430/japro.1484041