Akkaraman ve Romanov Kuzularının Alveolar Makrofajlarında Lipopolisakkarit ve Lipoteikoik Asite Yanıtta İmmün İlişkili Genlerin Ekspresyon Profilinin Araştırılması

Abstract

Alveolar makrofajlar (AM'lar), akciğerlerde bağışıklık tepkisini başlatan yabancı maddelere karşı savunma bariyeridir. Lipopolisakkarit (LPS) ve lipoteikoik asit (LTA) tehdidine yanıt olarak alveolar makrofajlardaki majör immün bağlantılı genlerin ekspresyon dinamiklerinin bilinmesi, çeşitli solunum yolu hastalıklarında yer alan hastalık mekanizmasının anlaşılmasına yardımcı olabilir. Bu çalışmanın amacı, koyun alveolar makrofajlarında lipopolisakkarit (LPS) ve lipoteikoik asit (LTA) stimülasyonuna yanıt olarak seçilen bağışıklıkla ilgili genlerin mRNA ekspresyonunu in vivo araştırmaktır. Sonuçlar, Romanov kuzularının TLR2, TLR4, NF-ĸβ, TNFa, IL-1β, IL-6, IL-8 ve IL-10 genlerinin mRNA ekspresyonunu Akkaraman kuzularına kıyasla kontrol grubuna göre daha yüksek saptandığını ortaya koymuştur. Ayrıca, her iki ırkta LPS ve LTA'nın kombine uygulanmasında TLR2, TLR4, NF-ĸβ, TNFa, IL-1β, IL-6, IL-8 ve IL-10 genlerinin ekspresyonu, LPS ve LTA'nın ayrı uygulamalarına kıyasla daha yüksektir cinste. Çalışmanın sonuçları, LPS ve LTA uygulamalarına yanıt olarak koyun AM'larında bağışıklıkla ilgili genlerin mRNA ekspresyonunun önemli ölçüde arttığını, LPS+LTS uygulamasında ise sinerjik bir etkinin gözlemlendiğini gösterdi. Ayrıca, ırk karşılaştırması, yerli Akkaraman'ın Romanov'a kıyasla hastalığa karşı daha dirençli olduğunu gösterdi.

Keywords

• sitokinler
• immunogenetik
• mRNA ekspresyonu
• solunum yolu hastalıkları
• koyun
• TLRs yolu

Project Number

FDK-2017-7657

Investigation on the Expression Profile of Immune-Related Genes in Response to Lipopolysaccharide and Lipoteichoic Acid in Alveolar Macrophages of Akkaraman and Romanov Lambs

Abstract

The alveolar macrophages (AMs) are frontier of defense against foreign materials that initiate immune response in lungs. Knowledge of the expression dynamics of major immune-related genes in the alveolar macrophages in response to lipopolysaccharide (LPS) and lipoteichoic acid (LTA) challenge can help to understand disease mechanism involved in several respiratory diseases. The aim of this study was to investigate the mRNA expression of selected immune-related genes in response to lipopolysaccharide (LPS) and lipoteichoic acid (LTA) challenge in sheep alveolar macrophages in vivo. Results revealed that Romanov lambs exhibited higher mRNA expression of TLR2, TLR4, NF-ĸβ, TNFα, IL-1β, IL-6, IL-8, and IL-10 genes as compared to Akkaraman lambs along with the control of all treatments. Moreover, the expression of TLR2, TLR4, NF-ĸβ, TNFα, IL-1β, IL-6, IL-8, and IL-10 genes was higher in combine treatment of LPS and LTA as compared to separate treatments of LPS and LTA in both breeds. The results showed that the mRNA expression of immune-related genes was significantly increased in the sheep AMs in response to LPS and LTA treatment whereas a synergistic effect was observed in LPS+LTS treatment. Also, breed comparison showed that the native Akkaraman was more resistant to disease compared to exotic Romanov.

Keywords

• Cytokines
• Immunogenetics
• mRNA expression
• Respiratory diseases
• Ovine
• TLRs pathway

Supporting Institution

Erciyes University Scientific Research Projects Unit

Project Number

FDK-2017-7657

Thanks

Authors are indebted to Agricultural Research-Application (ERÜTAM) Center at Erciyes University for providing animal facilities and Betül-Ziya Eren Genome and Stem Cell Research Center at Erciyes University (GENKÖK) for providing laboratory space and equipment. The authors are also thankful to the staff of the Department of Veterinary Genetics at Erciyes University for their help during animal experiments and sample collection. This project was supported by Erciyes University Scientific Research Projects Unit under the code of FDK-2017-7657.

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