Phylogenetic Analysis of Abaza, Kaçkar, Georgian (Caucasian), Ovit Region (İspir) Native Goat Breeds Using mtDNA D-loop Sequences

Year 2020, Volume: 3 Issue: 2, 176 - 188, 27.12.2020

Sadrettin Yüksel , Mehmet Ali Yıldız , Fatma Yüksel , Erdoğan Sezgin

Abstract

The objective of this research was to determine genetic diversity of mtDNA D-Loop region in Abaza, Kaçkar, Georgian (Caucasian) and Ovit Region (İspir) breeds and their phylogenetic relationship with each other. The number of goat have decreased gradually due to many reasons in some regions of Turkey. Thus, non-scientific hypotheses about goats began to be produced. Therefore, the origin and gene flow of native goat breeds reared in Eastern of Turkey are needed to reveal by using DNA based methods. In this study, 200 individuals from four native breeds in eastern of Turkey were sampled for the mtDNA D-loop sequences. The haplotype and nucleotide diversities were estimated as 0.974 and 0.019, respectively. In total, 52 haplotypes were observed from 63 polymorphic sites in native goat populations. Only one haplogroup A were found in studied populations. With the participation of all sequences, an neighbor-joining tree of native breeds was constructed. The lowest genetic distances (0.011) was observed between Kaçkar and Ovit Region (İspir) which have relatively close genetic relationships.

Keywords

Abaza Goat, Georgian (Caucasian) Goat, Haplotype, Kaçkar Goat, Mitochondrial D-Loop, Ovit Region (İspir) Goat

Abaza, Kaçkar, Gürcü (Kafkas), Ovit Bölgesi (İspir) Yerli Keçi Irklarının mtDNA D-Loop Dizileri Kullanılarak Filogenetik Analizi

Abstract

Bu araştırmanın amacı, Abaza, Kaçkar, Gürcü (Kafkas) ve Ovit Bölgesi (İspir) keçi ırklarında mtDNA D-Loop bölgesinin genetik çeşitliliğini ve birbirleriyle olan filogenetik ilişkilerini belirlemektir. Türkiye'nin bazı bölgelerinde birçok nedenden dolayı keçi sayısı giderek azalmış, böylece keçiler hakkında bilimsel olmayan hipotezler üretilmeye başlanmıştır. Bu doğrultuda, Türkiye'nin Doğu ve Kuzeydoğu bölgelerinde yetiştirilen yerli keçi ırklarının kökeni ve gen akışının, DNA temelli yöntemler kullanılarak ortaya konması önemli bilgilerin elde dilmesine yardımcı olacaktır. Bu çalışmada, dört yerli ırktan 200 baş keçi mtDNA D-loop dizileri için örneklenmiştir. Haplotip ve nükleotid çeşitliliği sırasıyla 0.974 ve 0.019 olarak tahmin edilmiştir. Yerli keçi popülasyonlarında 63 polimorfik bölgeden toplam 52 haplotip gözlenmiştir. İncelenen popülasyonlarda sadece bir A haplogrubu bulunmuştur. Tüm sekansların katılımıyla, neighbor-joining tree (NJT) oluşturulmuştur. En düşük genetik uzaklıklar (0,011), Kaçkar ve Ovit Bölgesi (İspir) arasında gözlemlenmiştir.

Keywords

Abaza Keçisi, Kaçkar Keçisi, Ovit Bölgesi Keçisi (İspir), Haplotip, Mitokondrial D-Loop

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