Determination of Appropriate Endogenous Reference Genes for RT-qPCR Analysis in Syrian (Golden) Hamsters and Mongolian Gerbils

Year 2024, , 533 - 542, 30.09.2024

Bülent Gündüz , Betül Önder , Tanay Uzgan

https://doi.org/10.30621/jbachs.1315023

Abstract

Purpose: The use of hamsters and gerbils has increased significantly in a variety of fields, including
biological rhythms, reproductive biology, immunology, oncology, and many others.
Material and Methods: The most stable genes in Syrian hamsters (Mesocricetus auratus) and
Mongolian gerbils (Meriones unguiculatus) were assessed using 32 reference genes for normalization
in RT-qPCR analysis. Adrenal, cerebral cortex, heart, hypothalamus, kidney, liver, lung and testis
tissues were used to extract and purify RNAs. GeNorm was used to determine the gene expression
stabilities of 14 candidate endogenous genes from each tissue that was compatible for both animals.

Results: Under our experimental conditions, we discovered that two endogenous genes are adequate
for each tissue to perform RT-qPCR normalization. There were differences in the most stable genes
between species and tissues.

Conclusion: We suggest that combinations of endogenous genes ought to be carefully chosen under
various experimental circumstances.

Keywords

hamsters, gerbils, reference genes, endogenous genes, qPCR

Supporting Institution

TÜBİTAK COST (European Cooperation in Science and Technology COST Association)

Project Number

Grant No: 111T639, 2011

Thanks

This research was supported by TÜBİTAK COST (European Cooperation in Science and Technology COST Association) (Grant No: 111T639, 2011)

Search for the Correct Endogenous Reference Genes by RT-qPCR in Syrian (Golden) Hamsters and Mongolian Gerbils

Abstract

Project Number

Grant No: 111T639, 2011

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