Search for the Correct Endogenous Reference Genes by RT-qPCR in Syrian (Golden) Hamsters and Mongolian Gerbils

Abstract

Determination of Appropriate Endogenous Reference Genes for RT-qPCR Analysis in Syrian (Golden) Hamsters and Mongolian Gerbils

Abstract

Purpose: The use of hamsters and gerbils has increased significantly in a variety of fields, including biological rhythms, reproductive biology, immunology, oncology, and many others. Material and Methods: The most stable genes in Syrian hamsters (Mesocricetus auratus) and Mongolian gerbils (Meriones unguiculatus) were assessed using 32 reference genes for normalization in RT-qPCR analysis. Adrenal, cerebral cortex, heart, hypothalamus, kidney, liver, lung and testis tissues were used to extract and purify RNAs. GeNorm was used to determine the gene expression stabilities of 14 candidate endogenous genes from each tissue that was compatible for both animals. Results: Under our experimental conditions, we discovered that two endogenous genes are adequate for each tissue to perform RT-qPCR normalization. There were differences in the most stable genes between species and tissues. Conclusion: We suggest that combinations of endogenous genes ought to be carefully chosen under various experimental circumstances.

Keywords

• hamsters
• gerbils
• reference genes
• endogenous genes
• qPCR

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